Characterization of the narrow-spectrum bacteriophage LSE7621 towards Salmonella Enteritidis and its biocontrol potential on lettuce and tofu

Liu, Aiping; Liu, Yilin; Lin, Pei; Cai, Xingzhe; Shen, Lihua; Duan, Maoping; Yu, Ning; Liu, Shuliang; Li, Chunyan; Liu, Yuntao; Chen, Hong; Wu, Wenjing; Wang, Xiaohong; Hu, Bin; Li, Cheng

doi:10.1016/j.lwt.2019.108791

Cited by 36 publications

(16 citation statements)

References 47 publications

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“…vB_SenM-1 also shows a high level of identity with other Salmonella phages such as SE13 (~96%) (NC_048763), yarpen (~95% identity) (NC_048863), brik (~95% identity) (NC_048864), and LSE7621 (~95% identity) (MK568062) (see also Figure 8 ). All of the phages are classified as belonging to the family Myoviridae, genus Rosemountvirus [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, phage LSE7621, to which phage vB_SenM-1 shows ~95% identity, has also been effectively tested in S. Enteritidis biocontrol on lettuce. However, this phage shows narrower host range than phage vB_SenM-1 [ 35 ]. Based on available data and experiments performed in this work, it can be assumed that phage vB_SenM-1 has the potential to be effectively used in food protection.…”

Section: Discussionmentioning

confidence: 99%

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Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

Kosznik-Kwaśnicka

Ciemińska

Grabski

et al. 2020

IJMS

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Molecular and functional characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, infecting various Salmonella enterica serovars and strains is presented. All these phages were able to develop lytically while not forming prophages. Moreover, they were able to survive at pH 3. The phages revealed different host ranges within serovars and strains of S. enterica, different adsorption rates on host cells, and different lytic growth kinetics at various temperatures (in the range of 25 to 42 °C). They efficiently reduced the number of cells in the bacterial biofilm and decreased the biofilm mass. Whole genome sequences of these phages have been determined and analyzed, including their phylogenetic relationships. In conclusion, we have demonstrated detailed characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, which reveal favorable features in light of their potential use in phage therapy of humans and animals, as well as for food protection purposes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

Kosznik-Kwaśnicka

Ciemińska

Grabski

et al. 2020

IJMS

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“…While these phages were tested in the same study, the extreme diversity of Salmonella serovars and strains makes it difficult to compare host ranges from different studies, especially since phage sensitivity may even be strain dependent. For example, phage LSE7621 (Rosemountvirus) was suggested to have a narrow host range, only infecting Salmonella Enteritidis [47]. However, LSE7621 was only tested on few strains from each of the 12 serovars included and may thus be able to infect a broader range of Salmonella serotypes, if tested on the same 34 Salmonella serovars used for phage SE13 [35,47].…”

Section: Discussionmentioning

confidence: 99%

“…For example, phage LSE7621 (Rosemountvirus) was suggested to have a narrow host range, only infecting Salmonella Enteritidis [47]. However, LSE7621 was only tested on few strains from each of the 12 serovars included and may thus be able to infect a broader range of Salmonella serotypes, if tested on the same 34 Salmonella serovars used for phage SE13 [35,47]. Yet, the host range of PA13076 [46] and SE13 [35] seems to confirm that the Rosemountvirus phages are able to infect many different Salmonella serovars, but no other genera.…”

Section: Discussionmentioning

confidence: 99%

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Gambino

Sørensen

Ahern

et al. 2020

IJMS

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Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter sakazakii. Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144 tail fiber structure is, overall, similar to the tail fiber of phage Mu and the C-terminus shows amino acid similarity to tail fibers of otherwise unrelated phages infecting Cronobacter. Since all phages in the Loughboroughvirus genus encode tail fibers similar to S144, we suggest that phages in this genus infect Cronobacter sakazakii and are polyvalent.

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“…The temperature stability of bacteriophage suspension in the SM buffer was tested at different temperatures (−20, 25, 37, 50, 60, and 70 • C) and incubated for 2 h. For pH stability, bacteriophage suspension was incubated at different pH ranges (pH 2, 3, 5, 7, 9, and 11) for 2 h. Bacteriophage suspension kept at 4 • C in the SM buffer (pH7.5) was used as a control in this study. After incubation, the titer of bacteriophage was determined by double agar overlay plaque assay and the survival rate was calculated by dividing the bacteriophage titer after treatment with the initial bacteriophage concentration in the control sample (Liu et al, 2020).…”

Section: Temperature and Ph Stabilitymentioning

confidence: 99%

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

et al. 2021

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Vibrio parahaemolyticus is a foodborne pathogen that is frequently isolated from a variety of seafood. To control this pathogenic Vibrio spp., the implementation of bacteriophages in aquaculture and food industries have shown a promising alternative to antibiotics. In this study, six bacteriophages isolated from the seafood samples demonstrated a narrow host range specificity that infecting only the V. parahaemolyticus strains. Morphological analysis revealed that bacteriophages Vp33, Vp22, Vp21, and Vp02 belong to the Podoviridae family, while bacteriophages Vp08 and Vp11 were categorized into the Siphoviridae family. All bacteriophages were composed of DNA genome and showed distinctive restriction fragment length polymorphism. The optimal MOI for bacteriophage propagation was determined to be 0.001 to 1. One-step growth curve revealed that the latent period ranged from 10 to 20 min, and the burst size of bacteriophage was approximately 17 to 51 PFU/cell. The influence of temperature and pH levels on the stability of bacteriophages showed that all bacteriophages were optimally stable over a wide range of temperatures and pH levels. In vitro lytic activity of all bacteriophages demonstrated to have a significant effect against V. parahaemolyticus. Besides, the application of a bacteriophage cocktail instead of a single bacteriophage suspension was observed to have a better efficiency to control the growth of V. parahaemolyticus. Results from this study provided a basic understanding of the physiological and biological properties of the isolated bacteriophages before it can be readily used as a biocontrol agent against the growth of V. parahaemolyticus.

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Characterization of the narrow-spectrum bacteriophage LSE7621 towards Salmonella Enteritidis and its biocontrol potential on lettuce and tofu

Cited by 36 publications

References 47 publications

Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

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