Characterization of the molecular degradation mechanism of diphenyl ethers by Cupriavidus sp. WS

Wang, Sheng; Bai, Naling; Wang, Bing; Feng, Zhuo; Hutchins, William; Yang, Ching‐Hong; Zhao, Yong

doi:10.1007/s11356-015-4854-3

Cited by 19 publications

(9 citation statements)

References 40 publications

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“…What is more, the very low 1,2-DO and 2,3-DO relative activity observed for this strain when single carbon sources were used might be correlated with the activation of different enzymes and degradation pathways. A similar observation was made by Wang et al [39] during the biodegradation of aromatic compounds by Cupriavidus sp. WS.…”

Section: Enzymatic Activitysupporting

confidence: 86%

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

et al. 2018

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The use of diphenyl ether (DE) and its 4-monohalogenated derivatives (4-HDE) as flame retardants, solvents, and substrates in biocide production significantly increases the risk of ecosystem contamination. Their removal is important from the point of view of environmental protection. The aim of this study was to evaluate the degradation processes of DE and 4-HDE by enzymes of the environmental bacterial strains under one-substrate and co-metabolic conditions. The study is focused on the biodegradation of DE and 4-HDE, the enzymatic activity of microbial strains, and the cell surface properties after contact with compounds. The results show that the highest biodegradation (96%) was observed for 4-chlorodiphenyl ether in co-metabolic culture with P. fluorescens B01. Moreover, the activity of 1,2-dioxygenase during degradation of 4-monohalogenated diphenyl ethers was higher than that of 2,3-dioxygenase for each strain tested. The presence of a co-substrate provoked changes in dioxygenase activity, resulting in the increased activity of 1,2-dioxygenase. Moreover, the addition of phenol as a co-substrate allowed for increased biodegradation of the diphenyl ethers and noticeable modification of the cell surface hydrophobicity during the process. All observations within the study performed have led to a deeper understanding of the contaminants’ biodegradation processes catalyzed by environmental bacteria.

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Section: Enzymatic Activitysupporting

confidence: 86%

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

et al. 2018

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“…It can metabolize 2,4,6-trichlorophenol, nitroaromatic compounds, phenol, trichloroethene, biaryl compounds, and PAHs. − Cupriavidus is a genus of Gram-negative bacteria that belongs to the family Burkholderiaceae. Similar to Ralstonia , Cupriavidus can metabolize various pollutants, including PAHs, benzylpenicillin, 2-and 4-nitrobenzoates, diphenyl ethers, and PCBs. − Although both Ralstonia and Cupriavidus are powerful degraders of organic pollutants based on traditional cultivation-based methods, their degradation capacity in the field is often questioned, challenging their application in bioaugmentation efforts. Hence, cultivation-independent approaches are necessary to identify functional microbes and confirm their functions in situ, but such studies are limited and have provided no direct evidence proving the PCB-degrading abilities of Ralstonia and Cupriavidus in soils.…”

Section: Discussionmentioning

confidence: 99%

Biphenyl-Metabolizing Microbial Community and a Functional Operon Revealed in E-Waste-Contaminated Soil

Jiang

Luo

Zhang

et al. 2018

Environ. Sci. Technol.

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Primitive electronic waste (e-waste) recycling activities release massive amounts of persistent organic pollutants (POPs) and heavy metals into surrounding soils, posing a major threat to the ecosystem and human health. Microbes capable of metabolizing POPs play important roles in POPs remediation in soils, but their phylotypes and functions remain unclear. Polychlorinated biphenyls (PCBs), one of the main pollutants in e-waste contaminated soils, have drawn increasing attention due to their high persistence, toxicity, and bioaccumulation. In the present study, we employed the culture-independent method of DNA stable-isotope probing to identify active biphenyl and PCB degraders in e-waste-contaminated soil. A total of 19 rare operational taxonomic units and three dominant bacterial genera ( Ralstonia, Cupriavidus, and uncultured bacterium DA101) were enriched in the C heavy DNA fraction, confirming their functions in PCBs metabolism. Additionally, a 13.8 kb bph operon was amplified, containing a bphA gene labeled byC that was concentrated in the heavy DNA fraction. The tetranucleotide signature characteristics of the bph operon suggest that it originated from Ralstonia. The bph operon may be shared by horizontal gene transfer because it contains a transposon gene and is found in various bacterial species. This study gives us a deeper understanding of PCB-degrading mechanisms and provides a potential resource for the bioremediation of PCBs-contaminated soils.

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“…Cupriavidus sp. WS degrades diphenyl ether, 4-bromodiphenyl ether, and 4,4 0 -bromodiphenyl ether (Wang et al 2015). Cupriavidus pinatubonensis JMP134 (formerly Ralstonia eutropha, Alcaligenes eutrophus, and Cupriavidus necator JMP134) degrades 2,4-dichlorophenoxyacetic Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of 2-butoxyethanol degrading bacterial strains

2020

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A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge, and identified by 16S rRNA gene sequence analysis. Eight of these strains belong to the genus Pseudomonas; the remaining three strains are Hydrogenophaga pseudoflava BOE3, Gordonia terrae BOE5, and Cupriavidus oxalaticus BOE300. In addition to 2-BE, all isolated strains were able to grow on 2-ethoxyethanol and 2-propoxyethanol, ethanol, n-hexanol, ethyl acetate, 2-butoxyacetic acid (2-BAA), glyoxylic acid, and n-butanol. Apart from the only gram-positive strain isolated, BOE5, none of the strains were able to grow on the nonpolar ethers diethyl ether, di-n-butyl ether, n-butyl vinyl ether, and dibenzyl ether, as well as on 1-butoxy-2-propanol. Strains H. pseudoflava BOE3 and two of the isolated pseudomonads, Pseudomonas putida BOE100 and P. vancouverensis BOE200, were studied in more detail. The maximum growth rates of strains BOE3, BOE100, and BOE200 at 30 °C were 0.204 h−1 at 4 mM, 0.645 h−1 at 5 mM, and 0.395 h−1 at 6 mM 2-BE, respectively. 2-BAA, n-butanol, and butanoic acid were detected as potential metabolites during the degradation of 2-BE. These findings indicate that the degradation of 2-BE by the isolated gram-negative strains proceeds via oxidation to 2-BAA with subsequent cleavage of the ether bond yielding glyoxylate and n-butanol. Since Gordonia terrae BOE5 was the only strain able to degrade nonpolar ethers like diethyl ether, the degradation pathway of 2-BE may be different for this strain.

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Characterization of the molecular degradation mechanism of diphenyl ethers by Cupriavidus sp. WS

Cited by 19 publications

References 40 publications

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

Bacterial Biodegradation of 4-Monohalogenated Diphenyl Ethers in One-Substrate and Co-Metabolic Systems

Biphenyl-Metabolizing Microbial Community and a Functional Operon Revealed in E-Waste-Contaminated Soil

Isolation and characterization of 2-butoxyethanol degrading bacterial strains

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