Isolation and characterization of 2-butoxyethanol degrading bacterial strains

Woiski, Christine; Dobslaw, Daniel; Engesser, Karl‐Heinrich

doi:10.1007/s10532-020-09900-3

Cited by 11 publications

(4 citation statements)

References 89 publications

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“…can tolerate high concentrations of aniline ( Mujahid et al, 2015 ), and Hydrogenophaga sp. can degrade 2-butoxyethanol ( Woiski et al, 2020 ) and might be responsible for microcystin degradation ( Ding et al, 2020 ). In contrast, in the CK group both on day 21 and 56, the predominant bacteria were Pseudomonas sp., Sphingobium sp., Novosphingobium sp., and Acidovorax sp.…”

Section: Resultsmentioning

confidence: 99%

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Chen

Zhang

et al. 2021

Front. Microbiol.

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Phenol is a toxic organic molecule that is widely detected in the natural environment, even in drinking water sources. Biological methods were considered to be a good tool for phenol removal, especially microbial immobilized technology. However, research on the “seed” bacteria along with microbial community analysis in oligotrophic environment such as drinking water system has not been addressed. In this study, Acinetobacter sp. DW-1 with high phenol degradation ability had been isolated from a drinking water biofilter was used as seeded bacteria to treat phenol micro-polluted drinking water source. Meanwhile, the whole genome of strain DW-1 was sequenced using nanopore technology. The genomic analysis suggests that Acinetobacter sp. DW-1 could utilize phenol via the β-ketoadipate pathway, including the catechol and protocatechuate branches. Subsequently, a bio-enhanced polyhedral hollow polypropylene sphere (BEPHPS) filter was constructed to investigate the stability of the seeded bacteria during the water treatment process. The denatured gradient gel electrophoresis (DGGE) profile and the quantification of phenol hydroxylase gene results indicate that when the BEPHPS filter was operated for 56 days, Acinetobacter sp. was still a persistent and competitive bacterium in the treatment group. In addition, 16S rRNA gene amplicon sequencing results indicate that Acinetobacter sp., as well as Pseudomonas sp., Nitrospira sp., Rubrivivax sp. were the predominant bacteria in the treatment group, which were different from that in the CK group. This study provides a better understanding of the mechanisms of phenol degradation by Acinetobacter sp. DW-1 at the gene level, and provides new insights into the stability of seeded bacteria and its effects on microbial ecology during drinking water treatment.

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Section: Resultsmentioning

confidence: 99%

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Chen

Zhang

et al. 2021

Front. Microbiol.

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“…The detection of 2-Butoxyethanol (2-BE) found in BW (Table 1), is mainly used as a solvent in surface coatings, paints, varnishes and lubricants, oils, and dyes [40]. Woiski et al [40] isolated 11 strains capable of entirely degrading 2-butoxyethanol (2-BE) isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge. Eight of these strains belong to the genus Pseudomonas.…”

Section: Gc-ms Analysis Of Real Bilge Watermentioning

confidence: 99%

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Gatidou

Drakou

Vyrides

2022

Water

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Bilge water is oily saline wastewater accumulated on the hull at the bottom of a vessel, generated from leakage from pipes and engines and wash-down freshwater containing cleaning solvents. The present study focused on isolating microorganisms from oil-contaminated sites and indigenous species from raw bilge water and assessment of their ability to biodegrade bilge water. Using phenanthrene as a carbon source Citrobacter species was isolated from oil-contaminated sites and its optimum growth condition was found. The results indicated significant tolerance of the bacterium which presented great biodegradation ability for the tested carbon source. At high salinity (33 g L−1 of NaCl), sufficient phenathrene removal was achieved (81%), whereas variation of pH from 5 to 10 did not affected the survival of the microorganism. Regarding the effect of temperature and nutrients, Citrobacter sp. was better adapted at 30 °C, while lack of nutrients presented a negative impact on its growth. Halomonas and Exiguobacterium sp. were isolated from real bilge water using phenanthrene and phenol as a carbon source. The isolated strains independently exposed to high and low range bilge water pointed out around 83% and 53% chemical oxygen demand (COD) removal, respectively. Analysis of untreated bilge water by gas chromatography-mass spectrometry (GC-MS) was carried out, and the results confirmed the presence of organic compounds having a high similarity with Heptane, N-hexadecanoic acid, Methyl isobutyl Ketone and 1-butoxy-2-propanol. Chromatographic analysis of treated bilge water after exposure to isolated strains indicated the existence of new compounds. These metabolites presented high similarity with N-hexadecanoic, methyl ester, N-hexadecanoic and Octadecanoic acid methyl ester.

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“…It is a trans-anethole derivation by degradation and oxidation reaction [21]. 2-Butoxyethanol or ethylene glycol monobutyl ether is a highly water-soluble ether and has low vapour pressure [22]. This compound mainly does not occur naturally, but produced by oxidation of butanol with ethylene oxide [23].…”

Section: A Essential Oil Extractionmentioning

confidence: 99%

Continuous Essential Oil and Oleoresin Extraction from Star Anise (Illicium verum) by Hydrodistillation and Solvent Extraction

Miranda

2022

IGTJ

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Star anise (Illicium verum) is widely used as a medicinal herb and spice. The extracts, essential oil and oleoresin, can be produced by hydro-distillation and steam distillation, while solvent and supercritical fluid extraction can be used to extract oleoresin. In this work, the star anise is distilled to obtain the oil, subsequently, its residue is extracted by solvent extraction to get the oleoresin. Whole and grounded star anise fruit is distilled by hydro-distillation for 8, 12, 16, 20, and 24 hours, thus the highest yield is obtained at 20 hours from grounded fruit, and the highest trans-anethole content of 68.50% is obtained at 8 hours from grounded fruit. Residue from the whole fruit-distillation process is extracted by Soxhlet extraction with three types of solvent (ethanol, diethyl ether, and n-hexane) for 6 hours. The highest yield was obtained from ethanol extraction with 1.16% yield and the highest content of trans-anethol was obtained from n-hexane fraction. One sample with the highest content of anethole from whole and grounded fruit-distilled oil and oleoresin are picked, and later they are tested to measure their antioxidant capacity by Ferric Reducing Antioxidant Power Assay (FRAP).

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Isolation and characterization of 2-butoxyethanol degrading bacterial strains

Cited by 11 publications

References 89 publications

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Assessment of Bilge Water Degradation by Isolated Citrobacter sp. and Two Indigenous Strains and Identification of Organic Content by GC-MS

Continuous Essential Oil and Oleoresin Extraction from Star Anise (Illicium verum) by Hydrodistillation and Solvent Extraction

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