Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth

Jiang, Pei; Ma, Xiangyu; Han, Shanying; Ma, Leyao; Ai, Jingru; Wu, Leilei; Zhang, Yuan; Xiao, Hairong; Tian, Mengyao; Tao, W. Andy; Zhang, Shasha; Chai, Renjie

doi:10.1007/s00018-022-04164-x

Cited by 11 publications

(5 citation statements)

References 177 publications

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“…In spite of the widely research about EVs in cancer and some other diseases, there are few studies to reveal the role of EVs in cochlea. Lately, ( Jiang et al (2022 ) has isolated cochlear tissue-derived EVs from mice of different ages to analyze and characterize the protein and miRNA contents of EVs. More than five hundred miRNAs and five thousand proteins have been detected in the EVs derived from cochlear.…”

Section: Other Origins Of Ti-evsmentioning

confidence: 99%

Research advances and challenges in tissue-derived extracellular vesicles

Zhi

Sun²,

Tang³

2022

Front. Mol. Biosci.

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Extracellular vesicles (EV) are vesicular vesicles with phospholipid bilayer, which are present in biological fluids and extracellular microenvironment. Extracellular vesicles serve as pivotal mediators in intercellular communication by delivering lipids, proteins, and RNAs to the recipient cells. Different from extracellular vesicles derived from biofluids and that originate from cell culture, the tissue derived extracellular vesicles (Ti-EVs) send us more enriched and accurate information of tissue microenvironment. Notably, tissue derived extracellular vesicles directly participate in the crosstalk between numerous cell types within microenvironment. Current research mainly focused on the extracellular vesicles present in biological fluids and cell culture supernatant, yet the studies on tissue derived extracellular vesicles are increasing due to the tissue derived extracellular vesicles are promising agents to reflect the occurrence and development of human diseases more accurately. In this review, we aimed to clarify the characteristics of tissue derived extracellular vesicles, specify the isolation methods and the roles of tissue derived extracellular vesicles in various diseases, including tumors. Moreover, we summarized the advances and challenges of tissue derived extracellular vesicles research.

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Section: Other Origins Of Ti-evsmentioning

confidence: 99%

Research advances and challenges in tissue-derived extracellular vesicles

Zhi

Sun²,

Tang³

2022

Front. Mol. Biosci.

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“…In addition, gene mutation in non-coding microRNAs (miRNAs) can also cause hearing loss. Indeed, many miRNAs have been characterized to affect the inner ear development of mice ( Jiang et al, 2022 ). miR-96 is the first microRNA discovered to be associated with progressive hearing loss in humans and mice ( Mencia et al, 2009 ; Solda et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

MiR-29a-deficiency causes thickening of the basilar membrane and age-related hearing loss by upregulating collagen IV and laminin

Wang

Geng

et al. 2023

Front. Cell. Neurosci.

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Age-related hearing loss (ARHL) is the most common sensory degenerative disease and can significantly impact the quality of life in elderly people. A previous study using GeneChip miRNA microarray assays showed that the expression of miR-29a changes with age, however, its role in hearing loss is still unclear. In this study, we characterized the cochlear phenotype of miR-29a knockout (miR-29a–/–) mice and found that miR-29a-deficient mice had a rapid progressive elevation of the hearing threshold from 2 to 5 months of age compared with littermate controls as measured by the auditory brainstem response. Stereocilia degeneration, hair cell loss and abnormal stria vascularis (SV) were observed in miR-29a–/– mice at 4 months of age. Transcriptome sequencing results showed elevated extracellular matrix (ECM) gene expression in miR-29a–/– mice. Both Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the key differences were closely related to ECM. Further examination with a transmission electron microscope showed thickening of the basilar membrane in the cochlea of miR-29a–/– mice. Five Col4a genes (Col4a1-a5) and two laminin genes (Lamb2 and Lamc1) were validated as miR-29a direct targets by dual luciferase assays and miR-29a inhibition assays with a miR-29a inhibitor. Consistent with the target gene validation results, the expression of these genes was significantly increased in the cochlea of miR-29a–/– mice, as shown by RT-PCR and Western blot. These findings suggest that miR-29a plays an important role in maintaining cochlear structure and function by regulating the expression of collagen and laminin and that the disturbance of its expression could be a cause of progressive hearing loss.

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“…Hearing loss is the most common sensorineural disorder affecting approximately 6.1% of the world population ( Qian et al, 2020 ; Zhang et al, 2021a ; Fu et al, 2021 ; Lv et al, 2021 ). It is estimated that more than half of the hearing loss cases are attributable to genetic factors ( He et al, 2017 ; Wang et al, 2022 ), while the other half of hearing loss cases could be caused by ototoxic drugs, such as aminoglycosides and anti-tumor drugs, aging, excessive noise exposure, and infections ( Li et al, 2018a ; Li et al, 2018b ; Liu et al, 2019a ; Cheng et al, 2019 ; Han et al, 2020 ; He et al, 2020 ; Zhong et al, 2020 ; Zhou et al, 2020 ; Liu et al, 2021a ; Zhang et al, 2021b ; Guo et al, 2021 ; He et al, 2021 ; Bu et al, 2022 ; Fu et al, 2022 ; Jiang et al, 2022 ). The functions of these hearing loss genes play an essential role in the development and function of hair cells and synaptic transmission of spiral ganglion neurons ( Wang et al, 2017 ; Zhu et al, 2018 ; Cheng et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…The functions of these hearing loss genes play an essential role in the development and function of hair cells and synaptic transmission of spiral ganglion neurons ( Wang et al, 2017 ; Zhu et al, 2018 ; Cheng et al, 2021 ). Thus, hearing loss is often induced by the loss of sensory hair cells and spiral ganglion neurons ( Liu et al, 2019b ; Guo et al, 2019 ; He et al, 2019 ; Qi et al, 2019 ; Chen et al, 2021 ; Hu et al, 2021 ; Wei et al, 2021 ; Guo et al, 2022 ; Hu et al, 2022 ; Jiang et al, 2022 ) in the inner ear cochlea. Up to 30/8/2021, at least 124 genes have been identified associated with non-syndromic hearing loss genes ( https://hereditaryhearingloss.org/ ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, PTPRQ protein forms a complex with myosin VI to tether the membrane of the stereocilia to stereocilia, causing reorganization of the actin cytoskeleton, and plays an important role in the mechanical transduction and adaptation of hair cells ( Takenawa and Itoh, 2001 ; Hirono et al, 2004 ; Sakaguchi et al, 2008 ). Studies in families with PTPRQ mutations show that mutants of the PTPRQ gene could cause autosomal recessive or autosomal dominant congenital sensorineural hearing loss, damage all frequency or high frequency, with or without vestibular dysfunction in infancy or early childhood ( Li et al, 2018a ; Li et al, 2018b ; Liu et al, 2019a ; Cheng et al, 2019 ; Zhou et al, 2020 ; Liu et al, 2021a ; Guo et al, 2021 ; He et al, 2021 ; Bu et al, 2022 ; Fu et al, 2022 ; Jiang et al, 2022 ). The hearing loss was progressive in some cases.…”

Section: Introductionmentioning

confidence: 99%

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Targeted Next-Generation Sequencing Identified Novel Compound Heterozygous Variants in the PTPRQ Gene Causing Autosomal Recessive Hearing Loss in a Chinese Family

et al. 2022

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Hearing loss is among the most common congenital sensory impairments. Genetic causes account for more than 50% of the cases of congenital hearing loss. The PTPRQ gene, encoding protein tyrosine phosphatase receptor Q, plays an important role in maintaining the stereocilia structure and function of hair cells. Mutations in the PTPRQ gene have been reported to cause hereditary sensorineural hearing loss. By using next-generation sequencing and Sanger sequencing, we identified a novel compound heterozygous mutation (c.997 G > A and c.6603-3 T > G) of the PTPRQ gene in a Chinese consanguineous family. This is the first report linking these two mutations to recessive hereditary sensorineural hearing loss. These findings contribute to the understanding of the relationship between genotype and hearing phenotype of PTPRQ-related hearing loss, which may be helpful to clinical management and genetic counseling.

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Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth

Cited by 11 publications

References 177 publications

Research advances and challenges in tissue-derived extracellular vesicles

Research advances and challenges in tissue-derived extracellular vesicles

MiR-29a-deficiency causes thickening of the basilar membrane and age-related hearing loss by upregulating collagen IV and laminin

Targeted Next-Generation Sequencing Identified Novel Compound Heterozygous Variants in the PTPRQ Gene Causing Autosomal Recessive Hearing Loss in a Chinese Family

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