Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus&lt;br&gt;Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus&lt;br&gt;doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

Junior, Edmar Maciel Lima; Bandeira, Tereza de Jesus Pinheiro Gomes; Miranda, Marcelo José Borges de; Ferreira, Guilherme Emilio; Parente, Ezequiel Aguiar; Piccolo, Nelson Sarto; Flho, Manoel Odorico de Moraes

doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

Cited by 17 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also a cheap and important source of type I collagen. Additionally, it was confirmed that it contains non-infectious microbiota 1 , and has a morphological structure homologous to the human skin 2 . Tilapia skin is also a highly available product, safe, innovative, and easy to apply materials.…”

Section: Introductionmentioning

confidence: 83%

See 1 more Smart Citation

The optimal concentration of silver nanoparticles in sterilizing fish skin grafts

Elshahawy

Mahmoud

Mokhtar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Collagen integrity should be considered on using a sterilizing agent for fish skin grafts. This study defined the optimal concentration of silver nanoparticles (Ag NPs) for sterilization of fish skin grafts without disrupting collagen content based on microbiological and histological evaluation. Strips of tilapia skin (n = 5) were randomly allocated to be immersed in Ag NPs solution at different concentrations of 25, 50, 100, and 250 µg/mL, respectively, for 5 min. The treated skin strips underwent bacteriological and histological evaluation. Yeast and fungi were more sensitive to Ag NPs than bacteria. On increasing the nanoparticles concentration, the total counts of aerobic bacteria decrease giving 933.3 ± 28.67, 601 ± 27.66, 288 ± 16.8, 15 ± 4.08 (CFU/cm2 ± S.D) at 25, 50, 100, and 250 µg/mL, respectively, comparing with untreated sample (1453.3 ± 57.92). Yeasts and filamentous fungi also exhibited a similar response, achieving a complete inhibition at 100 and 250 µg/mL. Bacillus cereus and Escherichia coli were the dominant aerobic bacteria, Candida albicans and Rhodotorula glutinis were the dominant aerobic yeasts, whereas Aspergillus niger, Aspergillus fumigatus, and Rhizopus stolonifer were the dominant aerobic fungi. The collagen fibers were loose with a wavey pattern at 25 µg/mL, wavey and slightly disorganized at 50 µg/mL, highly disorganized at 100 µg/mL, and compactly arranged and slightly loose at 250 µg/mL. Ag NPs at a concentration of 250 µg/mL could be considered a reliable and feasible method for the sterilization of fish skin grafts before application on human skin with an effective antimicrobial effect and less disrupting impact on collagen content.

show abstract

Section: Introductionmentioning

confidence: 83%

“…Tilapia skin has been used as an occlusive dressing for burn and wound healing because of its high content of collagen and homogeneity to human skin [1][2][3] . Tilapia skin grafts cannot be applied to the skin before being sterilized.…”

Section: Discussionmentioning

confidence: 99%

The optimal concentration of silver nanoparticles in sterilizing fish skin grafts

Elshahawy

Mahmoud

Mokhtar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Sterilization of skin grafts with irradiation worsened the structure of the skin matrix since it can cause collagen matrix cross-linking or breakage of the peptide bonds within the collagen molecules (22). Unlike mammalian skin grafts, which require harsh chemical sterilization, Tilapia skin grafts require a gentle processing method because the colony forming units found in the tilapia skin samples suggested the existence of a normal, non-infectious microbiota (23). However, the fish skin may be contaminated with different microorganisms, such as Pseudomonas aeruginosa, Aeromonas sobria, Klebsiella pneumonie, Enterococus faecalis, Aeromonas hydrophila, Proteus mirabilis, Globicatella sanguinis, Aeromonas veronii, Streptococcus uberis, Candida parapsilosis, and Streptococcus suis from water environment or other sources of contamination (23,24).…”

Section: Introductionmentioning

confidence: 99%

Validation of Three Different Sterilization Methods of Tilapia Skin Dressing: Impact on Microbiological Enumeration and Collagen Content

et al. 2020

View full text Add to dashboard Cite

Tilapia fish skin has demonstrated promise as a stable and practical biological dressing to be used in wound and burn management. However, the appropriate sterilization technique of the Tilapia fish skin is crucial before its clinical application. The standard sterilization technique must eliminate harmful pathogens but maintain the structural and biochemical properties that could compromise the dressing function. This study investigated and compared the efficiency of three sterilizing agents; chlorhexidine gluconate 4% (CHG), povidone iodine 10% (PVP-I), and silver nanoparticles (25 μg/mL) (AgNPs), at three different times (5, 10, and 15 min) on Tilapia fish skin based on the microbial count, histological and collagen properties. Among the sterilization procedures, AgNPs showed rapid and complete antimicrobial activity, with a 100% reduction in microbial growth of the fish skin throughout the treated times. Furthermore, AgNPs did not impair the cellular structure or collagen fibers content of the fish skin. However, CHG and PVP-I caused alterations in the collagen content. This study demonstrated that the AgNPs treatment of Tilapia fish skin provided sterile skin while preserving the histological properties and structural integrity. These findings provide an efficient and quick sterilization method suitable for Tilapia fish skin that could be adopted as a biological dressing.

show abstract

“…11,18 During the characterization of its microbiota, the quantified colony-forming units suggested normal, noninfectious colonization. 19 Also, a lack of variations in microscopic and tensiometric characteristics was seen after chemical sterilization with glycerol and gamma-irradiation up to 30 kGy. 11 When applied to experimental burns in rats, Nile tilapia fish skin led to faster reepithelialization, with no relevant changes in hematologic or biochemical parameters.…”

Section: Plastic and Reconstructive Surgery • May 2021mentioning

confidence: 95%

Nile Tilapia Fish Skin–Based Wound Dressing Improves Pain and Treatment-Related Costs of Superficial Partial-Thickness Burns: A Phase III Randomized Controlled Trial

Júnior

Filho

Costa

et al. 2021

Plastic &Amp; Reconstructive Surgery

View full text Add to dashboard Cite

Background: In a phase II study comparing Nile tilapia fish skin to silver sulfadiazine cream for outpatient management of superficial partial-thickness burns, the fish skin decreased reepithelialization time (average reduction, 1.43 days), dressing changes (average reduction, 3.72 dressings), and visual analogue scale pain scores. The present study aimed to further evaluate Nile tilapia fish skin efficacy for superficial partial-thickness burns. Unlike silver sulfadiazine cream, the fish skin has good adherence to the wound bed, which may prevent infections and decrease need for dressing changes. Thus, it could be a low-cost alternative to hasten healing and improve pain of burn patients. Methods: A phase III randomized controlled trial was conducted from April of 2017 to October of 2018 in Fortaleza, Brazil, and included 115 outpatients aged 18 to 70 years with superficial partial-thickness burns affecting 15 percent or less of body surface area and no previous treatment. Fifty-seven patients were treated with the glycerolized fish skin and 58 with silver sulfadiazine cream 1%. Primary outcomes were reepithelialization time, number of dressings, treatment-related costs, and pain intensity, assessed by means of visual analogue scale, Electronic von Frey, Burns Specific Pain Anxiety Scale, and analgesic use. Patients were evaluated every 48 hours. Results: Patients treated with fish skin required fewer days for reepithelialization (9.7 ± 0.6 days versus 10.2 ± 0.9 days; p = 0.001) and fewer dressings (1.6 ± 0.7 versus 4.9 ± 0.5; p < 0.001). They also had decreased analgesic needs and visual analogue scale, Burns Specific Pain Anxiety Scale, and Electronic von Frey measurements. Finally, fish skin use reduced the final average treatment-related cost per patient by 42.1 percent. Conclusion: By hastening reepithelialization, improving burn-related pain, and decreasing treatment-related costs, Nile tilapia fish skin could benefit the resource-poor public health systems of developing countries. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, II.

show abstract

Characterization of the microbiota of the skin and oral cavity of Oreochromis niloticus<br>Caracterização da microbiota da pele e cavidade oral de Oreochromis niloticus<br>doi:10.12662/2317-3076jhbs.v4i3.767.p193-197.2016

Cited by 17 publications

References 0 publications

The optimal concentration of silver nanoparticles in sterilizing fish skin grafts

The optimal concentration of silver nanoparticles in sterilizing fish skin grafts

Validation of Three Different Sterilization Methods of Tilapia Skin Dressing: Impact on Microbiological Enumeration and Collagen Content

Nile Tilapia Fish Skin–Based Wound Dressing Improves Pain and Treatment-Related Costs of Superficial Partial-Thickness Burns: A Phase III Randomized Controlled Trial

Contact Info

Product

Resources

About