Characterization of the mature form of a β-defensin-like peptide, Hoa-D1, in the lobster Homarus americanus

Vu, Giap H.; Do, Daniel; Rivera, Cindy D.; Dickinson, Patsy S.; Christie, Andrew E.; Stemmler, Elizabeth A.

doi:10.1016/j.molimm.2018.07.004

Cited by 7 publications

(13 citation statements)

References 44 publications

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“…In spite of a significant primary sequence homology with the C-terminal domain of big defensins, panusins are completely devoid of the N-terminal region and more closely resemble the architecture of vertebrate β-defensins. A similar sequence was recently identified in another decapod crustacean, the lobster Homarus americanus (29). In stark contrast with the scarce number of reports in Ecdysozoa, big defensins have been found on multiple occasions in Lophotrochozoa.…”

Section: Preamblesupporting

confidence: 70%

Functional Insights From the Evolutionary Diversification of Big Defensins

et al. 2020

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Big defensins are antimicrobial polypeptides believed to be the ancestors of β-defensins, the most evolutionary conserved family of host defense peptides (HDPs) in vertebrates. Nevertheless, big defensins underwent several independent gene loss events during animal evolution, being only retained in a limited number of phylogenetically distant invertebrates. Here, we explore the evolutionary history of this fascinating HDP family and investigate its patchy distribution in extant metazoans. We highlight the presence of big defensins in various classes of lophotrochozoans, as well as in a few arthropods and basal chordates (amphioxus), mostly adapted to life in marine environments. Bivalve mollusks often display an expanded repertoire of big defensin sequences, which appear to be the product of independent lineage-specific gene tandem duplications, followed by a rapid molecular diversification of newly acquired gene copies. This ongoing evolutionary process could underpin the simultaneous presence of canonical big defensins and non-canonical (β-defensin-like) sequences in some species. The big defensin genes of mussels and oysters, two species target of in-depth studies, are subjected to gene presence/absence variation (PAV), i.e., they can be present or absent in the genomes of different individuals. Moreover, big defensins follow different patterns of gene expression within a given species and respond differently to microbial challenges, suggesting functional divergence. Consistently, current structural data show that big defensin sequence diversity affects the 3D structure and biophysical properties of these polypeptides. We discuss here the role of the N-terminal hydrophobic domain, lost during evolution toward β-defensins, in the big defensin stability to high salt concentrations and its mechanism of action. Finally, we discuss the potential of big defensins as markers for animal health and for the nature-based design of novel therapeutics active at high salt concentrations.

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Section: Preamblesupporting

confidence: 70%

Functional Insights From the Evolutionary Diversification of Big Defensins

et al. 2020

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“…The only difference between these latter two proteins is a substitution of F 37 S 38 with S 37 F 38 . The final homolog, Panusin-like1, corresponds to the previously identified Hoa-D1 isolated from H. americanus granulocytic hemocytes (49).…”

Section: Highlighted Immune Genes In the H Americanus Genomementioning

confidence: 76%

“…This is significant because only two -defensin-like AMPs have previously been found in Ecdysozoa, one in Panurilus spp. (48) and one in H. americanus (49). These five genes encode three different proteins where Panusin-like4 and Panusin-like3 encode an identical protein, while Panusin-like2 and Panusin-like5 encode another identical protein.…”

Section: Highlighted Immune Genes In the H Americanus Genomementioning

confidence: 99%

“…A minimum of two high-quality reference sequences were selected from the Swiss-Prot curated protein database for each of the genes in the genome stability/DNA metabolism and chemical defense candidate lists for inclusion in a custom BLAST protein database. The custom database for the immune-related homology search was generated with all immunerelated transcripts from Clark and Greenwood (76) and the protein sequence of H. americanus panusin (49). The custom database for the neuronal gene complement included reference sequences for the pannexin/innexin family previously described in Moroz and Kohn (78); phenylalanine hydroxylase, tryptophan hydroxylase, tyrosine hydroxylase, carnitine palmitoyltransferase, choline acetyltransferase, sodium channel (SCNN1/ASSC), embryonic lethal abnormal vision mRNA processing (ELAV), and CUGBP-ELAV reference sequences previously described in Moroz and Kohn (77); and the C. elegans neuronal gene complement (79).…”

Section: Targeted Analysis Of Genes Associated With Neurology Immunit...mentioning

confidence: 99%

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The American lobster genome reveals insights on longevity, neural, and immune adaptations

et al. 2021

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The American lobster, Homarus americanus, is integral to marine ecosystems and supports an important commercial fishery. This iconic species also serves as a valuable model for deciphering neural networks controlling rhythmic motor patterns and olfaction. Here, we report a high-quality draft assembly of the H. americanus genome with 25,284 predicted gene models. Analysis of the neural gene complement revealed extraordinary development of the chemosensory machinery, including a profound diversification of ligand-gated ion channels and secretory molecules. The discovery of a novel class of chimeric receptors coupling pattern recognition and neurotransmitter binding suggests a deep integration between the neural and immune systems. A robust repertoire of genes involved in innate immunity, genome stability, cell survival, chemical defense, and cuticle formation represents a diversity of defense mechanisms essential to thrive in the benthic marine environment. Together, these unique evolutionary adaptations contribute to the longevity and ecological success of this long-lived benthic predator.

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“…The mature peptides are then post-translationally modified by a C-terminal amide. 16,115 These β-defensin-like peptides are mainly produced by granule-containing haemocytes 16 and are active against both Gram-positive and Gram-negative bacteria and yeast even at high salt concentrations. 115 Interestingly, the panusin member Hoa-D1 from the American lobster Homarus americanus 16 appears to be the same peptide (homarin) that was previously identified by Battison et al 20 At that time, the H. americanus homarin peptide was only partially characterised and showed activity against ciliated protozoans and a Vibrio strain isolated from lobster intestinal contents.…”

Section: Panusins (β-Defensin-like Peptides)mentioning

confidence: 99%

On the silver jubilee of crustacean antimicrobial peptides

Matos

Rosa

2021

Reviews in Aquaculture

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Antimicrobial peptides (AMPs) are widely distributed effector molecules that provide a first line of defence for organisms that lack adaptive immunity, such as crustaceans.They also represent bioactive molecules with great potential as therapeutic agents in aquaculture and biomedicine. Over the last years, an intensive research effort has been devoted to the identification and characterisation of antimicrobials in crustaceans resulting in the recognition of twelve gene-encoded AMP families to date. In addition to classical linear and multi-domain AMPs, crustaceans also rely on the production of unconventional ones, such as lysozymes and AMPs encrypted in large proteins carrying nonimmune functions. Besides their molecular and functional diversity, crustaceans have a particular AMP repertoire usually composed of diverse multigene families of taxonomically restricted peptides/polypeptides. In fact, only a few families of AMPs are shared between crustaceans and their close arthropod relatives. This review celebrates the silver jubilee of the discovery of the first crustacean AMPs and presents an updated and comprehensive overview in terms of structure, diversity and biological properties, emphasising their role in immune responses and in host-microbe interactions. We also discuss the main progress achieved so far and major challenges remaining.

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Characterization of the mature form of a β-defensin-like peptide, Hoa-D1, in the lobster Homarus americanus

Cited by 7 publications

References 44 publications

Functional Insights From the Evolutionary Diversification of Big Defensins

Functional Insights From the Evolutionary Diversification of Big Defensins

The American lobster genome reveals insights on longevity, neural, and immune adaptations

On the silver jubilee of crustacean antimicrobial peptides

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