Characterization of the main colonizer and biogenic pigments present in the red biofilm from La Galea Fortress sandstone by means of microscopic observations and Raman imaging

Morillas, Héctor; Maguregui, Maite; Marcaida, Iker; Trebolazabala, Josu; Salcedo, Isabel; Madariaga, Juan Manuel

doi:10.1016/j.microc.2015.02.005

Cited by 34 publications

(7 citation statements)

References 40 publications

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“…To highlight, we observed that the Raman spectra acquired for isolate Ap45E (which forms light-pink colonies) is remarkably similar to the spectra acquired for the Halobacterium salinarum NCR-1, a microorganism for which the Raman signal is due to bacterioruberin, represented by low stretching values for all peaks, when compared with other carotenoids ( Jehlička et al, 2014b ). Bacterioruberin is an unusual C50 carotenoid constantly studied and analyzed by Raman spectroscopy ( Imperi et al, 2007 ; Morillas et al, 2015 ), and present in some halophilic archaea and some actinobacteria belonging to the family Rubro bacteraceae ( Saito et al, 1994 ; Imperi et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media

et al. 2017

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Uncultured microorganisms comprise most of the microbial diversity existing on our planet. Despite advances in environmental sequencing and single-cell genomics, in-depth studies about bacterial metabolism and screening of novel bioproducts can only be assessed by culturing microbes in the laboratory. Here we report uncultured, or recalcitrant, microorganisms from an Antarctic soil sample, using relatively simple methods: oligotrophic media, extended incubation periods, observation under stereo microscopy, and selection of slow-growing bacteria. We managed to isolate several rare microorganisms belonging to infrequently isolated or recently described genera, for example Lapillicoccus, Flavitalea, Quadrisphaera, Motilibacter, and Polymorphobacter. Additionally, we obtained isolates presenting 16S rRNA sequence similarity ranging from 92.08 to 94.46% with any other known cultured species, including two distinct isolates from the class Thermoleophilia, that although common in Antarctic soils (as identified by metagenomics), was never reported to be isolated from such samples. Our data indicates that simple methods are still useful for cultivating recalcitrant microorganisms, even when dealing with samples from extreme environments.

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Section: Resultsmentioning

confidence: 99%

Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media

et al. 2017

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“…5 ), exhibited several bands and a complex shape, due to the contribution of several biomolecules; specific patterns of bands represent the fingerprint of specific biomolecules and/or functional groups. The lipid pigments extracted from purple samples were assigned to: bacteriorhodopsin (yellow area), isoprenoid derivatives (green area) and carotenoid and bacterioruberin (a 50-carbon open chain carotenoid; orange area) 17 . Raman spectra of bacteriorhodopsin or rhodopsin-like pigments were dominated by the signal of retinal isomers, whose dominant feature in the Raman spectra is a complex strong band in the 1575 cm −1 region.…”

Section: Resultsmentioning

confidence: 99%

Purple spot damage dynamics investigated by an integrated approach on a 1244 A.D. parchment roll from the Secret Vatican Archive

Migliore

Thaller

Vendittozzi

et al. 2017

Sci Rep

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Ancient parchments are commonly attacked by microbes, producing purple spots and detachment of the superficial layer. Neither standard cultivation nor molecular methods (DGGE) solved the issue: causative agents and colonization model are still unknown. To identify the putative causal agents, we describe the 16 S rRNA gene analysis (454-pyrosequencing) of the microbial communities colonizing a damaged parchment roll dated 1244 A.D. (A.A. Arm. I-XVIII 3328, Vatican Secret Archives). The taxa in damaged or undamaged areas of the same document were different. In the purple spots, marine halotolerant Gammaproteobacteria, mainly Vibrio, were found; these microorganisms are rare or absent in the undamaged areas. Ubiquitous and environmental microorganisms were observed in samples from both damaged and undamaged areas. Pseudonocardiales were the most common, representing the main colonizers of undamaged areas. We hypothesize a successional model of biodeterioration, based on metagenomic data and spectroscopic analysis of pigments, which help to relate the damage to a microbial agent. Furthermore, a new method (Light Transmitted Analysis) was utilized to evaluate the kind and entity of the damage to native collagen. These data give a significant advance to the knowledge in the field and open new perspectives to remediation activity on a huge amount of ancient document.

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“…Here, the occurrence of microorganisms can lead to different pathologies including esthetic and structural damage due to physical and chemical alterations. Thus, organic colonizers can lead to hue alterations on artworks, such as the pink/orange‐stained inner walls of the Évora Cathedral in Portugal (biofilm of Rhodotorula ) or the reddish facade from the north side of the La Galea fortress in Spain (biofilm of algae and cyanobacteria) . In both examples, the main biogenic pigments could be identified via Raman spectroscopy on mortar microfragments and sandstone samples.…”

Section: Raman Spectroscopy For the Detection Of Bacteria In Environmmentioning

confidence: 99%

The application of Raman spectroscopy for the detection and identification of microorganisms

Stöckel

Kirchhoff

Neugebauer

et al. 2015

J Raman Spectroscopy

204

155

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A fast and reliable detection and identification of microorganisms is crucial in environmental science, for food quality as well as medical diagnosis. In these fields, all types of Raman spectroscopy are gaining more and more importance during the last years. The review provides an extensive overview of recent research, technical expertise, and scientific findings based on Raman spectroscopic detection and identification of microorganisms within the years 2010 and 2015, demonstrating the diverse capability of Raman spectroscopy as a modern analytical tool. Raman spectroscopy distinguishes itself from other currently applied techniques by its easy application at low cost, its high speed of analysis, and its broad information content on both the chemical composition and the structure of biomolecules within the microorganisms. Slight chances in the chemical composition of microorganisms can be monitored by means of Raman spectroscopy and used to differentiate genera, species, or even strains. Detection of pathogens is possible from complex matrices, such as soil, food, and body fluids. Further, spectroscopic studies of host–pathogen interactions are addressed as well as the effect of antibiotics on bacteria. Copyright © 2015 John Wiley & Sons, Ltd.

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Characterization of the main colonizer and biogenic pigments present in the red biofilm from La Galea Fortress sandstone by means of microscopic observations and Raman imaging

Cited by 34 publications

References 40 publications

Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media

Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media

Purple spot damage dynamics investigated by an integrated approach on a 1244 A.D. parchment roll from the Secret Vatican Archive

The application of Raman spectroscopy for the detection and identification of microorganisms

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