Characterization of the lipase activity of<i>Malassezia furfur</i>

Plotkin, Lilian I.; Squiquera, Luis; Mathov, Irina; Galimberti, Ricardo; Leoni, Juliana

doi:10.1080/02681219680000071

Cited by 45 publications

(31 citation statements)

References 19 publications

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“…Ran et al (1993) did not detect lipases in the supernatant, but determined that the main lipolytic activity was in the insoluble fraction of cell extracts. Contrary to these results, Plotkin et al (1996) demonstrated lipolytic activity in the supernatant and in intracellular soluble and insoluble extracts of M. furfur, and further characterized three different lipolytic activities in the soluble fraction.…”

Section: Introductionmentioning

confidence: 54%

MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur

Brunke

Hube

2006

Microbiology

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Malassezia furfur is a dimorphic fungus and a member of the normal cutaneous microflora of humans. However, it is also a facultative pathogen, associated with a wide range of skin diseases. One unusual feature of M. furfur is an absolute dependency on externally provided lipids which the fungus hydrolyses by lipolytic activity to release fatty acids necessary for both growth and pathogenicity. In this study, the cloning and characterization of the first gene encoding a secreted lipase of M. furfur possibly associated with this activity are reported. The gene, MfLIP1, shows high sequence similarity to other known extracellular lipases, but is not a member of a lipase gene family in M. furfur. MfLIP1 consists of 1464 bp, encoding a protein with a molecular mass of 54?3 kDa, a conserved lipase motif and an N-terminal signal peptide of 26 aa. By using a genomic library, two other genes were identified flanking MfLIP1, one of them encoding a putative secreted catalase, the other a putative amine oxidase. The cDNA of MfLIP1 was expressed in Pichia pastoris and the biochemical properties of the recombinant lipase were analysed. MfLip1 is most active at 40 6C and the pH optimum was found to be 5?8. The lipase hydrolysed lipids, such as Tweens, frequently used as the source of fatty acids in M. furfur media, and had minor esterase activity. Furthermore, the lipase is inhibited by different bivalent metal ions. This is the first molecular description of a secreted lipase from M. furfur.

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Section: Introductionmentioning

confidence: 54%

MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur

Brunke

Hube

2006

Microbiology

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“…contributes to skin inflammation in SD, Plotkin in 1996 and De Angelis in 2007 described the production of a lipase by this fungus that is essential for its growth in vitro and in vivo. 69,70 The hypothesis would be that the fungus uses lipids from the skin surface to produce unsaturated and saturated fatty acids that, when left in the individual's skin milieu induce an inflammatory response. 71,72 The sebum in the skin permits the growth of P. ovale (i.e.…”

Section: Malassezia Sppmentioning

confidence: 99%

Dermatite seborreica

Sampaio

Mameri²,

Vargas

et al. 2011

An. Bras. Dermatol.

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Seborrheic dermatitis is a chronic relapsing erythematous scaly skin disease, the prevalence of which is around 1 to 3% of the general population in the United States. It has two incidence peaks, the first in the first three months of life and the second beginning at puberty and reaching its apex at 40 to 60 years of age. The prevalence of seborrheic dermatitis is higher in HIV-positive individuals and the condition tends to be more intense and refractory to treatment in these patients. Neurological disorders and other chronic diseases are also associated with the onset of seborrheic dermatitis. The currently accepted theory on the pathogenesis of this disease advocates that yeast of Malassezia spp., present on the skin surface of susceptible individuals, leads to a non-immunogenic irritation due to the production of unsaturated fatty acids deposited on the skin surface. This article provides a review of the literature on seborrheic dermatitis, focusing on immunogenetics, the clinical forms of the disease and its treatment. . Keywords: Dermatitis; Dermatitis, seborrheic; Eczema Resumo: A dermatite seborreica é uma doença eritêmato-escamativa de caráter crônico-recidivante que acomete entre 1 e 3% da população geral dos Estados Unidos. Possui dois picos de incidência -o primeiro, durante os três primeiros meses de vida, e o segundo, a partir da puberdade, atingindo seu ápice entre os 40 e 60 anos de idade. Os indivíduos HIV positivos têm maior prevalência da doença, que apresenta maior intensidade e tendência à refratariedade ao tratamento. Doenças neurológicas e outras doenças crônicas também estão associadas ao desenvolvimento da dermatite seborreica. Como mecanismo fisiopatogênico, reconhece-se que o fungo Malassezia sp., presente na pele de indivíduos suscetíveis, leve a uma irritação não-imunogênica a partir da produção de metabólitos à base de áci-dos graxos insaturados deixados na superfície cutânea. Este artigo faz uma revisão da literatura sobre dermatite seborreica, com ênfase nos aspectos imunogenéticos, formas clínicas e tratamento.

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“…The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent (13,21,33 Temperature conditions such as growth at 37ºC and production of enzymes, including lipases, phospholipases and proteases are associated with pathogenicity (10,28,39).…”

mentioning

confidence: 99%

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

Neves¹,

Magalhães²,

Silva³

et al. 2005

Braz. J. Microbiol.

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This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates. Key words: Malassezia furfur, M. simpodialis, pathogenicityMalassezia is a lipophilic yeast frequently found in normal human skin. Current evidence indicates a high rate of skin colonization in healthy adults, in contrast with the low rate of colonization in prepubertal children (20).Gueho et al. (11) (14,17,35,38). With the exception of M. pachidermatis, the remaining eight species have an absolute in vitro requirement for suplementation of long-chain fatty acids. Based on studies conducted within the last 5 years, it appears that the most frequently organism associated with pityriasis versicolor may not be M. furfur and possible candidates include M. globosa (2,27) and M. sympodialis (15). The genus Malassezia was recently revised, but the clinical significance of each of these species is not clearly understood (15).Pityriasis versicolor (tinea versicolor) is a superficial fungal infection of the skin, appearing most commonly on the upper trunk, as well as on the upper arms, neck and face. As suggested by its name, pityriasis versicolor may be manifested in different colours, ranging from pink or to dark brown or even black. The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent (13,21,33).

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Characterization of the lipase activity ofMalassezia furfur

Cited by 45 publications

References 19 publications

MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur

MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur

Dermatite seborreica

Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules

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