Characterization of the Inhibition Effect Induced by
Nickel on Glucose-6-Phosphate Dehydrogenase and
Glutathione Reductase

Cartañà, J.; Arola, Lluı́s; Romeu, A

doi:10.1159/000469044

Cited by 8 publications

(3 citation statements)

References 2 publications

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“…Glutathione metabolizing enzymes play an impotant role in the protection of cells against oxidative and alkylating agents. Cartana et al (1992) similarly observed BIOTRANSFORMATION OF TNT IN TIGER SALAMANDERS organ speci"c activity of GSH-GR in rats, which may relate to the di!erent susceptibilities of di!erent organs to a toxic compound. However, there were no signi"cant di!erences between control and TNT-exposed salamanders in liver, kidneys, skin, and lungs.…”

Section: Discussionmentioning

confidence: 73%

Fate and the Biochemical Effects of 2,4,6-Trinitrotoluene Exposure to Tiger Salamanders (Ambystoma tigrinum)

Johnson

Vodela

Reddy

et al. 2000

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 73%

Fate and the Biochemical Effects of 2,4,6-Trinitrotoluene Exposure to Tiger Salamanders (Ambystoma tigrinum)

Johnson

Vodela

Reddy

et al. 2000

Ecotoxicology and Environmental Safety

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“…The increased risk of cold welding and the high production costs of titanium on one hand, and the release of metal ions such as nickel by 316L steel on the other hand created needs to search for alternative alloys. Besides causing allergic reactions in patients, nickel has also been shown to inhibit the catalytic activity of enzymes (for example catalase [30] and glutathione reductase [31]). Referring to bone physiology, it has been demonstrated that catalytic activity of ALP is inhibited by metal ions like aluminum or nickel [32,33].…”

Section: Discussionmentioning

confidence: 99%

P2000 - A high-nitrogen austenitic steel for application in bone surgery

et al. 2019

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Optimal treatment of bone fractures with minimal complications requires implant alloys that combine high strength with high ductility. Today, TiAl6V4 titanium and 316L steel are the most applied alloys in bone surgery, whereas both share advantages and disadvantages. The nickel-free, high-nitrogen austenitic steel X13CrMnMoN18-14-3 (1.4452, brand name: P2000) exhibits high strength in combination with superior ductility. In order to compare suitable alloys for bone implants, we investigated titanium, 316L steel, CoCrMo and P2000 for their biocompatibility and hemocompatibility (according to DIN ISO 10993–5 and 10993–4), cell metabolism, mineralization of osteoblasts, electrochemical and mechanical properties. P2000 exhibited good biocompatibility of fibroblasts and osteoblasts without impairment in vitality or changing of cell morphology. Furthermore, investigation of the osteoblasts function by ALP activity and protein levels of the key transcription factor RUNX2 revealed 2x increased ALP activity and more than 4x increased RUNX2 protein levels for P2000 compared to titanium or 316 steel, respectively. Additionally, analyses of osteoblast biomineralization by Alizarin Red S staining exhibited more than 6x increased significant mineralization of osteoblasts grown on P2000 as compared to titanium. Further, P2000 showed no hemolytic effect and no significant influence on hemocompatibility. Nanoindentation hardness tests of Titanium and 316L specimens exposed an indentation hardness (HIT) of about 4 GPa, whereas CoCrMo and P2000 revealed HIT of 7.5 and 5.6 GPa, respectively. Moreover, an improved corrosion resistance of P2000 compared to 316L steel was observed. In summary, we could demonstrate that the nickel-free high-nitrogen steel P2000 appears to be a promising alternative candidate for applications in bone surgery. As to nearly all aspects like biocompatibility and hemocompatibility, cell metabolism, mineralization of osteoblasts and mechanical properties, P2000 was similar to or revealed advantages against titanium, 316L or CoCrMo.

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“…Toxic effects of Ni 2+ at intracellular sites are numerous and comprise inhibition of catalase,42 glutathion reductase, and glutathion peroxidase43, 44 all of which could lower the ability of cells to protect against reactive oxygen species 3. In higher concentrations (>500 μM) Ni 2+ can also cause apoptosis, histone hypoacetylation and cell cycle arrest 45.…”

Section: Discussionmentioning

confidence: 99%

Uptake of nickel from 316L stainless steel into contacting osteoblastic cells and metal ion interference with BMP‐2‐induced alkaline phosphatase

Mölders

Felix

Bingmann

et al. 2007

J Biomedical Materials Res

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Bone cells contacting nickel (Ni)-containing implant materials may be affected by Ni species via disturbed signaling pathways involved in bone cell development. Here we analyze effects of the Ni-containing steel 316L and major metal constituents thereof on bone morphogenetic protein-2 (BMP-2)-induced alkaline phosphatase (ALP) of MC3T3-E1 cells. While cells grew normally on 316L, cellular Ni content increased 10-fold vs. control within 4 days. With respect to the major components of 316L, Ni2+ (3-50 microM) was most inhibitory to BMP-2-induced ALP, whereas even 50 microM Fe3+, Cr3+, Mo5+, or Mn2+ had no such effect. In line with this, BMP-2-induced ALP was significantly reduced in cells on 316L. This effect was not prevented by the metal ion chelator diethylenetriaminepentaacetic acid (DTPA). Instead, DTPA abolished the stimulatory effect of BMP-2 on ALP, pointing to chelatable metal ions involved. Zn2+, as one possible candidate, antagonized the Ni2+ inhibition of BMP-2-induced ALP in both MC3T3-E1 and human bone marrow stromal cells. Results show that cells contacting 316L steel are exposed to increased concentrations of Ni which suffice to impair BMP-2-induced ALP activity. Zn2+, as a competitor of this inhibition, may help to restore normal osteoblastic function and bone development under these conditions.

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Characterization of the Inhibition Effect Induced by Nickel on Glucose-6-Phosphate Dehydrogenase and Glutathione Reductase

Cited by 8 publications

References 2 publications

Fate and the Biochemical Effects of 2,4,6-Trinitrotoluene Exposure to Tiger Salamanders (Ambystoma tigrinum)

Fate and the Biochemical Effects of 2,4,6-Trinitrotoluene Exposure to Tiger Salamanders (Ambystoma tigrinum)

P2000 - A high-nitrogen austenitic steel for application in bone surgery

Uptake of nickel from 316L stainless steel into contacting osteoblastic cells and metal ion interference with BMP‐2‐induced alkaline phosphatase

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