Characterization of the <i>crotalus durissus terrificus</i> venom by atomic force microscopy

Baranauskas, Vı́tor; Dourado, Doroty Mesquita; Jingguo, Zhao; Cruz‐Höfling, Maria Alice da

doi:10.1116/1.1486007

Cited by 2 publications

(4 citation statements)

References 12 publications

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“…As opposed to other methods which are used to image the Type I collagen ultrastructure of tissues, samples imaged using AFM remain intact to a greater degree, increasing confidence that measured properties are sample characteristics rather than artifacts of processing or imaging . Although AFM has been used by other groups to image the collagen matrix in mineralized dentin and bone, − and other nonmineralized tissues, − the current study uses a quantitative and statistically powered technique to analyze the nanoscale morphology of individual Type I collagen fibrils in multiple tissues from the same animal.…”

Section: Introductionmentioning

confidence: 99%

Type I Collagen Exists as a Distribution of Nanoscale Morphologies in Teeth, Bones, and Tendons

et al. 2010

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This study demonstrates that collagen, the most abundant protein in animals, exists as a distribution of nanoscale morphologies in teeth, bones, and tendons. This fundamental characteristic of Type I collagen has not previously been reported and provides a new understanding of the nanoscale architecture of this ubiquitous and important biological nanomaterial. Dentin, bone and tendon tissue samples were chosen for their differences in cellular origin and function, as well as to compare mineralized tissues with a tissue which lacks mineral in a normal physiological setting. A distribution of morphologies was present in all three tissues, confirming that this characteristic is fundamental to Type I collagen regardless of the presence of mineral, cellular origin of the collagen (osteoblast versus odontoblast versus fibroblast), anatomical location or mechanical function of the tissue.

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Section: Introductionmentioning

confidence: 99%

Type I Collagen Exists as a Distribution of Nanoscale Morphologies in Teeth, Bones, and Tendons

et al. 2010

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“…The venom protein composition explains pharmaceutical effects and clinical manifestations of the South American rattlesnake envenomation. Neurotoxic pathological effects, systemic myonecrosis, coagulopathy, myoglobinuria and acute renal failure are the major clinical features observed after accidents by C. d. terrificus . , …”

Section: Resultsmentioning

confidence: 99%

“…Neurotoxic pathological effects, systemic myonecrosis, coagulopathy, myoglo- binuria and acute renal failure are the major clinical features observed after accidents by C. d. terrificus. 26,35 Phospholipases A 2 . The venom proteome analysis revealed high quantities and predominance of PLA 2 s, which are responsible for the neurotoxicity and failure of the neuromuscular junctions.…”

Section: -D Gel Electrophoresis Of the C D Terrificusmentioning

confidence: 99%

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Snake Venomic of Crotalus durissus terrificus—Correlation with Pharmacological Activities

et al. 2010

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The snake venomic of Crotalus durissus terrificus was analyzed by 2-D and 1-D electrophoresis and subsequent MS/MS and enzymatic assays. The venomic of the South American rattlesnake comprises toxins from seven protein families: phospholipases A(2), serine proteinases, ecto-5'-nucleotidases, metalloproteinases, nerve growth factors, phosphodiesterases, and glutaminyl cyclase. The venom toxin composition correlates with the clinical manifestation of the crotalinae snake bites and explains pathological effects of the venom such as neurotoxicity, systemic myonecrosis, hemostatic disorders, myoglobinuria, and acute renal failure. The vast majority of toxins are potentially involved in neurotoxicity, myotoxicity, and coagulopathy. The predominant venom components are neurotoxic phospholipases A(2) and serine proteinases. The venom is a rich source of 5'-nucleotidases (7.8% of the identified toxins) inducing hemostatic disorders. Analysis of the venom protein composition provided a catalogue for secreted toxins. The venomic composition of Crotalus d. terrificus and venom gland transcriptome of the synonymous subspecies Crotalus d. collilineatus show differences in the occurrence of protein families and in the abundance of toxins. Some of the venom components identified by the proteomic analysis were not reported in the transcriptome of the Crotalus d. collilineatus venom gland. Enzymatic activities of the Crotalus d. terrificus venom were determined and correlated with the proteomic composition.

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Characterization of the crotalus durissus terrificus venom by atomic force microscopy

Cited by 2 publications

References 12 publications

Type I Collagen Exists as a Distribution of Nanoscale Morphologies in Teeth, Bones, and Tendons

Type I Collagen Exists as a Distribution of Nanoscale Morphologies in Teeth, Bones, and Tendons

Snake Venomic of Crotalus durissus terrificus—Correlation with Pharmacological Activities

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