Characterization of the hypothalamic proopiomelanocortin gene and β-endorphin expression in the hypothalamic arcuate nucleus of mice elicited by inflammatory pain

Seo, Young-Jun; Kwon, Min-Soo; Choi, Seong-Soo; Han, Eun‐Jung; Jung, Jun‐Sub; Choi, Ho-Kyew; Park, S.-H.; Jang, Jeong-Eun; Suh, Hwal

doi:10.1016/j.neuroscience.2007.06.047

Cited by 18 publications

(12 citation statements)

References 54 publications

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“…β-Endorphin is synthesized by the cells in the arcuate nucleus of the hypothalamus and the nucleus tractus solitarius in the brain stem (Bronstein et al, 1992), which contain neurons projecting widely throughout the brain. Recent studies have reported that the hypothalamic POMC, which is a precursor of β-endorphin, is significantly increased by noxious stimuli (Sun et al, 2003;Seo et al, 2008). In addition to the behavioural results, we observed that single and repeated immobilization stress itself significantly increased hypothalamic POMC gene expression and that the hypothalamic POMC gene expression that was induced by inflammatory nociceptive stimuli was also altered by pre-treatment of a single or subchronic (7 times repetition) immobilization stress (Fig.…”

Section: Discussionsupporting

confidence: 74%

Differential cross-tolerance development between single and repeated immobilization stress on the antinociceptive effect induced by β-endorphin, 5-hydroxytryptamine, morphine, and WIN55,212-2 in the inflammatory mouse pain mode

Seo¹,

Kwon²,

Choi

et al. 2011

Arch. Pharm. Res.

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We have evaluated the possible underlying mechanisms of immobilization stress-induced analgesia (SIA) by behavioral cross-tolerance studies and molecular studies. In the behavioral studies, the cross-tolerance between single or repeated immobilization SIA and the antinociceptive effects of β-endorphin, morphine, 5-hydroxytryptamine (5-HT), or WIN55,212-2 were assessed. Both single and repeated (×7) immobilization stress significantly attenuated the β-endorphin and 5-hydroxytryptamine-induced antinociception in the 2nd phase of formalin response, respectively. However, these cross-tolerances disappeared in prolonged repetition of the stress (×14). Neither single nor repeated (×7 and ×14) immobilization stress affected the antinociceptive effect of morphine or WIN55,212-2 at all. We also found that immobilization stress activated hypothalamic proopiomelanocortin (POMC) gene and β-endorphin expression. Since, it has potent inhibitory activity on the noxious stimuli-induced POMC expression, immobilization stress seemed to dissipate the POMC gene expression process. Meanwhile, we did not find any changes in the opioid receptors' (mu-, delta- and kappa-receptor) and the cannabinoid receptors' (CB1 and CB2) expressions in the midbrain regions elicited by single or repeated stress. These results suggested that a single immobilization stress activates the descending pain modulatory system, which is mainly mediated through endorphinergic and serotonergic activation. Moreover, the tolerance of SIA induced by repeated stresses may be due to the prolonged activation of these systems induced by repeated immobilization.

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Section: Discussionsupporting

confidence: 74%

Differential cross-tolerance development between single and repeated immobilization stress on the antinociceptive effect induced by β-endorphin, 5-hydroxytryptamine, morphine, and WIN55,212-2 in the inflammatory mouse pain mode

Seo¹,

Kwon²,

Choi

et al. 2011

Arch. Pharm. Res.

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“…This observation is consistent with the fact that insect development is suppressed transiently during diapause. Proopiomelanocortin is the precursor of a number of biologically active peptides in the pituitary of animals, and its expression is regulated by several signal regulatory molecules in the cytoplasm and nucleus (Seo et al, 2008). Our results showed that this protein was markedly less abundant during and after diapause, suggesting that this protein may be connected with diapause related signal transduction.…”

Section: Discussionmentioning

confidence: 62%

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Cheng¹,

Li²,

Yu³

et al. 2009

Eur. J. Entomol.

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Abstract. To determine the relationship between protein expression and insect diapause, a proteomic approach was used to investigate the proteins extracted from larvae of the wheat blossom midge Sitodiplosis mosellana Gehin at different developmental stages, including pre-diapause, over-summering diapause, over-wintering diapause and post-diapause. Using 2-DE gels stained with coomassie brilliant blue, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of the other stages. There were 91, 92 and 95 protein spots that showed more than a 2-fold change in abundance in the over-summering diapause, over-wintering diapause and post-diapause stages compared with pre-diapause. Eight protein spots, which showed the greatest difference in the larvae at different stages of diapause, were analyzed using Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Seven of them were successfully identified from their peptide mass fingerprints using the NCBInr database. They were proopiomelanocortin, NADH dehydrogenase subunit 1 and F10F2.5, which were up-regulated or unique to pre-diapause larvae, IKK interacting protein isoform 2 up-regulated in diapause and post-diapause larvae, GA10647-PA unique in over-wintering diapause larvae, purple CG16784-PB isoform B and B0228.6 up-regulated in over-summering and overwintering diapause larvae. The potential functions of these proteins during wheat blossom midge diapause are discussed.

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“…injection of PD98059 attenuated formalin-induced increase of POMC mRNA expression in the hypothalamus. These results indicate that ERK1/2 activation in the hypothalamus may contribute to neuroendocrine regulation [24]. ERK1/2 activation in the hypothalamic paraventricular nucleus (PVN) has also been reported after intraplantar formalin or i.t.…”

Section: Activation Of Mitogen-activated Protein Kinase In Descendmentioning

confidence: 95%

“…This neural circuit has been implicated in the production of stimulation-produced and stress-induced analgesia [1, 2, 91]. Formalin injection into the hindpaw activated ERK1/2 in the hypothalamus [24]. ERK1/2 activation markedly increased at 30 min and remained higher than baseline after 24 h. p-ERK1/2 was colocalized with beta-endorphin in the Arc neurons.…”

Section: Activation Of Mitogen-activated Protein Kinase In Descendmentioning

confidence: 99%

Activation of Mitogen-Activated Protein Kinase in Descending Pain Modulatory System

Imbe

Senba

Kimura

et al. 2011

Journal of Signal Transduction

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The descending pain modulatory system is thought to undergo plastic changes following peripheral tissue injury and exerts bidirectional (facilitatory and inhibitory) influence on spinal nociceptive transmission. The mitogen-activated protein kinases (MAPKs) superfamily consists of four main members: the extracellular signal-regulated protein kinase1/2 (ERK1/2), the c-Jun N-terminal kinases (JNKs), the p38 MAPKs, and the ERK5. MAPKs not only regulate cell proliferation and survival but also play important roles in synaptic plasticity and memory formation. Recently, many studies have demonstrated that noxious stimuli activate MAPKs in several brain regions that are components of descending pain modulatory system. They are involved in pain perception and pain-related emotional responses. In addition, psychophysical stress also activates MAPKs in these brain structures. Greater appreciation of the convergence of mechanisms between noxious stimuli- and psychological stress-induced neuroplasticity is likely to lead to the identification of novel targets for a variety of pain syndromes.

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Characterization of the hypothalamic proopiomelanocortin gene and β-endorphin expression in the hypothalamic arcuate nucleus of mice elicited by inflammatory pain

Cited by 18 publications

References 54 publications

Differential cross-tolerance development between single and repeated immobilization stress on the antinociceptive effect induced by β-endorphin, 5-hydroxytryptamine, morphine, and WIN55,212-2 in the inflammatory mouse pain mode

Differential cross-tolerance development between single and repeated immobilization stress on the antinociceptive effect induced by β-endorphin, 5-hydroxytryptamine, morphine, and WIN55,212-2 in the inflammatory mouse pain mode

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Activation of Mitogen-Activated Protein Kinase in Descending Pain Modulatory System

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