Characterization of the genome of the murine papovavirus K

Abstract

The DNA genome of the murine papovavirus K virus (KV) was characterized and compared with the genome of polyoma virus. A physical map of the KV genome was constructed by analysis of the size of DNA fragments generated by sequential cleavage with combinations of restriction endonucleases. By using one of the three EcoRI sites in the KV genome as the 0 map position, the KV physical map was then oriented to the polyoma virus genome. Of 42 restriction sites mapped within the KV genome, 7 were localized within 0.01 map unit of their respective sites in the polyoma virus genome; an eighth site mapped within 0.02 map unit. KV replication was examined and found to be bidirectional, initiating at approximately 0.70 map unit. This corresponds weli to the origin of replication within the polyoma virus genome and further supports the orientation of the KV physical map.

Conserved polynucleotide sequences among the genomes of papillomaviruses

“…Restriction endonucleases BamHI, HincII, HindIll, HpaI, EcoRI, and BglI were purchased from New England Biolabs, Lowell, Mass., or from Bethesda Research Laboratories, Rockville, Md. Each viral DNA (1 ytg) was digested with 2 U of restriction endonucleases in 100 ,l of the appropriate buffer at 37°C for 1 h. Sequential cleavage of viral DNAs was achieved as previously described (16).…”

Complete Genome Sequence of Murine Pneumotropic Virus ( Polyomaviridae ) Clone pKV(37-1)

“…We sequenced the plasmid, pKV(37-1), encoding the entire MPtV genome inserted into the PstI site of pBR322, obtained from the American Type Culture Collection (ATCC) ( 7 ), as the plasmid sequence was not previously reported. The plasmid was propagated in and isolated from Escherichia coli using standard molecular biology techniques ( 8 ) and was directly sequenced without prior PCR amplification.…”

Analysis of T antigen and viral DNA in mouse cells transformed by K virus, a nononcogenic murine papovavirus

“…This result supported the interpretation that integrated KV DNA consisted of tamdemly repeated full-length KV DNA. BglI cleaves KV DNA at two sites and generates 2.9-and 2.1-kbp fragments (8). These two fragments were detected after digestion of the DNA with BglI; a prominent 5.1-kbp band and three bands of integrated viral DNA with flanking cellular sequences were also observed.…”