Analysis of T antigen and viral DNA in mouse cells transformed by K virus, a nononcogenic murine papovavirus

Kanda, Tadahito; Takemoto, Kenneth K.

doi:10.1128/jvi.50.3.954-956.1984

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…Based on studies in vivo, the cellular tropism of MPtV has been claimed to be limited to vascular endothelial cells and renal tubuli, whereas MPyV infects a wide range of cell types (Dawe et al, 1987). However, this conclusion must be taken with caution because MPtV has been less characterized than MPyV (Greenlee & Law, 1985;Kanda & Takemoto, 1984). In vitro studies on MPtV have been hampered, since the virus has been very difficult to replicate in vitro and, moreover, less interest has been focused on MPtV because it is non-tumourigenic (Greenlee & Law, 1985;Kanda & Takemoto, 1984).…”

Section: Introductionmentioning

confidence: 99%

“…However, this conclusion must be taken with caution because MPtV has been less characterized than MPyV (Greenlee & Law, 1985;Kanda & Takemoto, 1984). In vitro studies on MPtV have been hampered, since the virus has been very difficult to replicate in vitro and, moreover, less interest has been focused on MPtV because it is non-tumourigenic (Greenlee & Law, 1985;Kanda & Takemoto, 1984). Nevertheless, it has been shown recently that the MPtV regulatory region influences cellular tropism, since a substitution of the MPtV regulatory region with a segment containing the MPyV transcriptional enhancer had a positive effect on viral DNA replication in cells that are normally non-permissive for MPtV (Zhang & Magnusson, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Murine pneumotropic virus VP1 virus-like particles (VLPs) bind to several cell types independent of sialic acid residues and do not serologically cross react with murine polyomavirus VP1 VLPs

Tegerstedt

Andreasson

Vlastos

et al. 2003

Journal of General Virology

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The ability of murine pneumotropic virus (MPtV) major capsid protein VP1 to form virus-like particles (VLPs) was examined. MPtV-VLPs obtained were used to estimate the potential of MPtV to attach to different cells and to assess some characteristics of the MPtV cell receptor. Furthermore, to evaluate if MPtV-VLPs could potentially complement murine polyomavirus (MPyV) VP1 VLPs (MPyV-VLPs) as vectors for prime-boost gene therapy, the capability of MPtV-VLPs to serologically cross react with MPyV-VLPs and to transduce DNA into cells was examined. MPtV VP1 obtained in a recombinant baculovirus system formed MPtV-VLPs readily. MPtV-VLPs were shown by FACS analysis to bind to different cells, independent of MHC class I antigen expression. In addition, MPtV-VLPs did not cause haemagglutination of red blood cells and MPtV-VLP binding to cells was neuraminidase resistant but mostly trypsin and papain sensitive, indicating that the MPtV receptor lacks sialic acid components. When tested by ELISA and in vivo neutralization assays, MPtV-VLPs did not serologically cross react with MPyV-VLPs, suggesting that MPtV-VLPs and MPyV-VLPs could potentially be interchanged as carriers of DNA in repeated gene therapy. Finally, MPtV-VLPs were shown to transduce foreign DNA in vitro and in vivo.In conclusion, the data suggest that MPtV-VLPs, and possibly also MPtV, bind to several different cell types, that binding is neuraminidase resistant and that MPtV-VLPs should potentially be able to complement MPyV-VLPs for prime-boost gene transfer in vivo.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Murine pneumotropic virus VP1 virus-like particles (VLPs) bind to several cell types independent of sialic acid residues and do not serologically cross react with murine polyomavirus VP1 VLPs

Tegerstedt

Andreasson

Vlastos

et al. 2003

Journal of General Virology

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Papovavirus Infections of the Nervous System

Greenlee

1989

Clinical and Molecular Aspects of Neurotropic Virus Infection

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Interaction of K papovavirus with hamster cells: Transformation of glial cellsin vitro but failure of the virus to produce central nervous system tumorsin vivo

Greenlee

Law

1985

Archives of Virology

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Primary cultures of lungs, kidneys, and glial cells derived from midgestation Syrian hamsters were inoculated with 10(5) hemagglutinating units of murine K-papovavirus and were serially subcultivated to allow appearance of lines of persistently infected or transformed cells. K virus did not replicate in renal cell cultures and produced only transient productive infection of lung cells. Evidence of K virus-induced cell transformation was not detected in either of these cultures. Inoculation of glial cultures with K virus, however, resulted initially in a protracted infection in which 80--100 percent of cells expressed K virus V antigen for 18 subcultivations and in which cloning experiments suggested that all cells in the culture contained the viral genome. After 18 subcultivations numbers of positive cells rapidly diminished, and cells appeared which exhibited altered morphology and density dependence. These altered cells (KVHG3 cells) grew well in serum-free media, could be cloned in soft agar, and were negative for infectious virus or K virus V antigen. Although KVHG3 cells did not exhibit staining when reacted with antisera to K virus T antigen, Southern blot analysis of these cultures demonstrated the presence of K virus DNA integrated into the host chromosomal DNA and indicated that some rearrangement of the viral genome had occurred. Attempts to produce tumors in hamsters with these cells were unsuccessful, as were attempts to induce tumors in newborn hamsters by intracranial inoculation of K virus. The present study demonstrates that K virus is capable of causing productive infection and cell transformation in primary cultures of fetal hamster glial cells but that other hamster cell types are relatively resistant to the virus and that both K virus and K virus-transformed hamster cells are poorly oncogenic for hamsters in vivo.

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Analysis of T antigen and viral DNA in mouse cells transformed by K virus, a nononcogenic murine papovavirus

Cited by 4 publications

References 11 publications

Murine pneumotropic virus VP1 virus-like particles (VLPs) bind to several cell types independent of sialic acid residues and do not serologically cross react with murine polyomavirus VP1 VLPs

Murine pneumotropic virus VP1 virus-like particles (VLPs) bind to several cell types independent of sialic acid residues and do not serologically cross react with murine polyomavirus VP1 VLPs

Papovavirus Infections of the Nervous System

Interaction of K papovavirus with hamster cells: Transformation of glial cellsin vitro but failure of the virus to produce central nervous system tumorsin vivo

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