Characterization of the Genetic Supports for Betalactam Resistance in Escherichia coli Strains of Porcine Origin Producing Extended-spectrum Beta-Lactamase (ESBL)

Kouadio, Innocent Kouamé; Guessennd, Nathalie; Dadié, Adjehi; Konaté, Ali; Gbonon, Valérie; Tiekoura, Bertin; Kpoda, S; Ouattara, Mohamed Baguy; Konan, Fernique; Ajayi, Adeyinka Olusegun; Djè, Marcellin Koffi; Dosso, Mireille

doi:10.9734/mrji/2017/38449

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sulfonamide resistance was also observed in this work with a rate of 65.23% for sulfamethoxazoletrimethoprim. These results are lower than those obtained by [22] [26] [30] and [31] and higher than those obtained by [32]. This prevalence of resistance to sulfamethoxazole-trimethoprim is thought to be due to the fact that it is one of the most commonly prescribed antibiotics for suspected bacterial diarrhea and the most financially accessible.…”

Section: Discussionmentioning

confidence: 55%

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Cissé¹,

Kagambèga²,

Bouda³

et al. 2023

AiM

View full text Add to dashboard Cite

Background: The emergence and spread of multidrug-resistant bacteria have become a major public health problem worldwide, particularly in developing countries such as Burkina Faso. This study aims to determine phenotypic and genotypic antibiotic resistant diarrheagenic Escherichia coli (DEC) from patients with diarrhea in Ouagadougou, Burkina Faso. Methodology: Microbiological and biochemical analysis were done to detect two hundred and ninety-two (292) strains. The susceptibility of the strains to antibiotics was determined by the agar disc diffusion method. 16-plex-PCR assays were carried out to detect both virulence and resistance genes encoding betalactams, quinolones, phenicols, tetracyclines and virulence gene of DEC. Results: Diarrheagenic Escherichia coli was detected in 8% (23/292) of patients with diarrhea using the 16-plex-PCR and 39.1% (9/23) of the DEC detected carry at least one resistance gene. Resistance rate in disc diffusion test was 86.96% to tetracycline, 65.23% to cotrimoxazole, 17.4% to nalidixic acid, 17.4% to norfloxacin, 17.4% to ciprofloxacin, 13.04% to ceftriaxone, 13.04% to cefotaxime, 8.7% to gentamicin, 8.7% to Chloramphenicol, 0% to netilmicin. The prevalence of different resistance genes in the studied strains varied from 44.4% to 5.5%. The gene Tet coding for resistance to tetracycline was found in 8 strains (44.4%). The CatA gene coding for resistance to Chloramphenicol was detected in 38.9% of isolates. The qnrS, bla SHV and bla OXA genes were each detected in 5.5% of isolates. No strain hosts the qnrA, qnrB and bla TEM genes. Conclusion: This study identified β-lactams, quinolones, phenicols and te-

show abstract

Section: Discussionmentioning

confidence: 55%

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Cissé¹,

Kagambèga²,

Bouda³

et al. 2023

AiM

View full text Add to dashboard Cite

show abstract

“…It is not surprising, because many studies reported urine as the major clinical source of E. coli (Abimiku et al, 2019;Adenipekun et al, 2016;Khoshbakht, Salimi, Aski, & Keshavarzi, 2013). In addition to that, E. coli has been reported as the predominant facultative anaerobe and the commensal microbiota in the mammalian GIT, thereby playing a key role in hospital and community-acquired infections (Kouadio et al, 2017;Nuhu et al, 2020). Although some reported factors led to the dominance of E. coli as urine pathogens.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial susceptibility pattern of Escherichia coli at University of Abuja Teaching Hospital: A Retrospective Study

GALADIMA

Ugwu²,

Olumeko³

et al. 2022

jcbr

View full text Add to dashboard Cite

Escherichia coli (E. coli), a normal gastro-intestinal microflora of humans and animals can be pathogenic causing a wide range of diseases. Rational use of antibiotics is one way of reducing antimicrobial resistance. This study retrospectively assessed the antimicrobial resistance patterns of E. coli from clinical samples at University of Abuja Teaching Hospital. Records of test results of microbial, culture and sensitivity from clinical samples from January 2016 to December 2019 were studied. A proforma was used to collect data from the record on antimicrobial sensitivity pattern of E. coli. Discrete and categorical variables were reported as frequencies and percentages. Requirements for the study were met in 91.7% of the data collected. Female clients 81.8% were more than their male counterparts. Urine samples have the highest number of E. coli 62%. This was followed by samples from palate swab 14%. Eye swab, vaginal swab, ascitic fluid and pleural fluid had one each (0.03%). The penicillin were mostly resisted by the isolates, while most were sensitive to the fluoroquinolones and cephalosporins. Summary of the resistance patterns from 2016 to 2019 showed most isolates were sensitive to the effect of antimicrobials used in 2016 and 2018, while most were resistant in 2017 and 2019. The urinary tract was the most common site of E. coli infection. The pattern of resistance was inconsistent across all antibiotic classes studied

show abstract

Characterization of the Genetic Supports for Betalactam Resistance in Escherichia coli Strains of Porcine Origin Producing Extended-spectrum Beta-Lactamase (ESBL)

Cited by 2 publications

References 0 publications

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Antimicrobial susceptibility pattern of Escherichia coli at University of Abuja Teaching Hospital: A Retrospective Study

Contact Info

Product

Resources

About

Characterization of the Genetic Supports for Betalactam Resistance in Escherichia coli Strains of Porcine Origin Producing Extended-spectrum Beta-Lactamase (ESBL)

Cited by 2 publications

References 0 publications

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Antimicrobial susceptibility pattern of Escherichia coli at University of Abuja Teaching Hospital: A Retrospective Study

Contact Info

Product

Resources

About

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic <i>Escherichia coli</i> Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso