Phenotypic and Genotypic Antibiotic Resistant Diarrheagenic &amp;lt;i&amp;gt;Escherichia coli&amp;lt;/i&amp;gt; Isolated from Patients with Diarrhea in Ouagadougou, Burkina Faso

Cissé, Haoua; Kagambèga, Assèta; Bouda, Soutongnooma Caroline; Sawadogo, Abdallah; Barro, Nicolas

doi:10.4236/aim.2023.137022

Cited by 1 publication

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies carried out in Ouagadougou, Burkina Faso, the prevalence of ETEC was low, ranging from 3.2% to 12.9% [19][20][21][22]. This prevalence is similar to that obtained via culture (followed by conventional PCR) in the present study, where a proportion of 4.2% was found.…”

Section: Discussionsupporting

confidence: 86%

Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic Escherichia coli (ETEC) and Shigella in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso

Héma,

Sermé,

Sawadogo

et al. 2023

Microorganisms

View full text Add to dashboard Cite

The estimates of enterotoxigenic Escherichia coli (ETEC) and Shigella burden in developing countries are limited by the lack of rapid, accessible, and sensitive diagnostics and surveillance tools. We used a “Rapid LAMP based Diagnostic Test (RLDT)” to detect ETEC and Shigella in diarrheal and non-diarrheal stool samples from a 12-month longitudinal cohort of children under five years of age in a peri-urban area of Ouagadougou in Burkina Faso (West Africa). To allow comparison with the RLDT-Shigella results, conventional culture methods were used to identify Shigella strains in the stool samples. As conventional culture alone cannot detect ETEC cases, a subset of E. coli-like colonies was tested using conventional PCR to detect ETEC toxins genes. Of the 165 stool samples analyzed for ETEC, 24.9% were positive when using RLDT against 4.2% when using culture followed by PCR. ETEC toxin distribution when using RLDT was STp 17.6% (29/165), LT 11.5% (19/165), and STh 8.5% (14/165). Of the 263 specimens tested for Shigella, 44.8% were positive when using RLDT against 23.2% when using culture. The sensitivity and specificity of the RLDT compared to culture (followed by PCR for ETEC) were 93.44% and 69.8% for Shigella and 83.7% and 77.9% for ETEC, respectively. This study indicates that both Shigella and ETEC are substantially underdiagnosed when using conventional culture and highlights the potential contribution of the new RLDT method to improve enteric disease burden estimation and to guide future efforts to prevent and control bacterial enteric infection and disease.

show abstract

Section: Discussionsupporting

confidence: 86%