Characterization of the Functional Changes in Mouse Gut Microbiome Associated with Increased <i>Akkermansia muciniphila</i> Population Modulated by Dietary Black Raspberries

Tu, Pengcheng; Bian, Xiaoming; Chi, Liang; Gao, Bei; Ru, Hongyu; Knobloch, Thomas J.; Weghorst, Christopher M.; Lü, Kun

doi:10.1021/acsomega.8b00064

Cited by 48 publications

(56 citation statements)

References 58 publications

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“…Genus Eggerthellaceae is associated with only one lipid metabolite ( Figure 5). Surprisingly, the abundance change of genus Akkermansia and Lactobacillus, the probiotic associated with anti-inflammation and multiple metabolic processes, are not related with any differential lipid metabolite in function ( Figure 5) (Tu et al, 2018). Similarly, genus Alloprevotella, genus Rikenellaceae, genus Muribaculaceae, genus Enterococcus and genus Lachnospiraceae are also not related with any differential lipid metabolite.…”

Section: Functional Correlation Analysis Between Gut Microbiome and Lmentioning

confidence: 93%

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Yan

Zhang

et al. 2020

Front. Cell. Infect. Microbiol.

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Radiation enteritis (RE) is a common complication in cancer patients receiving radiotherapy. Although studies have shown the changes of this disease at clinical, pathological and other levels, the dynamic characteristics of local microbiome and metabolomics are hitherto unknown. We aimed to examine the multi-omics features of the gut microecosystem, determining the functional correlation between microbiome and lipid metabolites during RE activity. By delivering single high-dose irradiation, a RE mouse model was established. High-throughput 16S rDNA sequencing and global lipidomics analysis were performed to examine microbial and lipidomic profile changes in the gut microecosystem. Spearman correlation analysis was used to determine the functional correlation between bacteria and metabolites. Clinical samples were collected to validate the above observations. During RE activity, the intestinal inflammation of the mice was confirmed by typical signs, symptoms, imaging findings and pathological evidences. 16S datasets revealed that localized irradiation dramatically altered the gut microbial composition, resulting in a decrease ratio of Bacteroidetes to Firmicutes. Lipidomics analysis indicated the remarkable lipidomic profile changes in enteric epithelial barrier, determining that glycerophospholipids metabolism was correlated to RE progression with the highest relevance. Spearman correlation analysis identified that five bacteriametabolite pairs showed the most significant functional correlation in RE, including Alistipes-PC(36:0e), Bacteroides-DG(18:0/20:4), Dubosiella-PC(35:2), Eggerthellaceae-PC(35:6), and Escherichia-Shigella-TG(18:2/18:2/20:4). These observations were partly confirmed in human specimens. Our study provided a comprehensive description of microbiota dysbiosis and lipid metabolic disorders in RE, suggesting strategies to change local microecosystem to relieve radiation injury and maintain homeostasis.

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Section: Functional Correlation Analysis Between Gut Microbiome and Lmentioning

confidence: 93%

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Yan

Zhang

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Su et al [ 80 ] reported that RB-derived pelargonidin-3- O -glucoside increased the gut population of Prevotella and improved the Bacteroidetes/Firmicutes ratio. Another more recent report concluded that there was a favorable higher population of Akkermansia muciniphila and Bacteroidetes/Firmicutes ratios in pathogenic free mice fed on black RB powder [ 81 ]. Conclusively, RB consumption showed antidiabetic effects, inhibiting glucosidase and amylase activities, strengthening the endogenous antioxidant defense system, reducing inflammatory biomarkers, activating AMPK, GLUT2/GLUT4, IRS-1 phosphorylation, downregulating lipogenesis and gluconeogenesis genes, and increasing epithelial mucus barrier protecting and SCOA-producing bacterial populations ( Table 1 ) [ 83 , 84 ].…”

Section: Raspberriesmentioning

confidence: 99%

Select Polyphenol-Rich Berry Consumption to Defer or Deter Diabetes and Diabetes-Related Complications

et al. 2020

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Berries are considered “promising functional fruits” due to their distinct and ubiquitous therapeutic contents of anthocyanins, proanthocyanidins, phenolic acids, flavonoids, flavanols, alkaloids, polysaccharides, hydroxycinnamic, ellagic acid derivatives, and organic acids. These polyphenols are part of berries and the human diet, and evidence suggests that their intake is associated with a reduced risk or the reversal of metabolic pathophysiologies related to diabetes, obesity, oxidative stress, inflammation, and hypertension. This work reviewed and summarized both clinical and non-clinical findings that the consumption of berries, berry extracts, purified compounds, juices, jams, jellies, and other berry byproducts aided in the prevention and or otherwise management of type 2 diabetes mellitus (T2DM) and related complications. The integration of berries and berries-derived byproducts into high-carbohydrate (HCD) and high-fat (HFD) diets, also reversed/reduced the HCD/HFD-induced alterations in glucose metabolism-related pathways, and markers of oxidative stress, inflammation, and lipid oxidation in healthy/obese/diabetic subjects. The berry polyphenols also modulate the intestinal microflora ecology by opposing the diabetic and obesity rendered symbolic reduction of Bacteroidetes/Firmicutes ratio, intestinal mucosal barrier dysfunction-restoring bacteria, short-chain fatty acids, and organic acid producing microflora. All studies proposed a number of potential mechanisms of action of respective berry bioactive compounds, although further mechanistic and molecular studies are warranted. The metabolic profiling of each berry is also included to provide up-to-date information regarding the potential anti-oxidative/antidiabetic constituents of each berry.

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“…We have shown that BRB feeding impacts the microbiome in murine models and enhances diversity. [ 19,63 ] However, how the gut microbiota impacts the metabolism of phytochemicals from a specific food is not well understood. It is likely that the individual microbial populations and their function contribute to the heterogeneity in ET metabolism.…”

Section: Discussionmentioning

confidence: 99%

Dose‐Dependent Increases in Ellagitannin Metabolites as Biomarkers of Intake in Humans Consuming Standardized Black Raspberry Food Products Designed for Clinical Trials

Roberts

Grainger

Thomas‐Ahner

et al. 2020

Molecular Nutrition Food Res

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Scope: Black raspberry (BRB) phytochemicals demonstrate anti-carcinogenic properties in experimental models, including prostate cancer. Two BRB foods, a confection and nectar, providing a consistent and reproducible product for human clinical studies are designed and characterized. Methods and results: Men with clinically localized prostate cancer are sequentially enrolled to a control group or one of four intervention groups (confection or nectar, 10 or 20 g dose; n = 8 per group) for 4 weeks prior to prostatectomy. Primary outcomes include: safety, adherence, and ellagitannin metabolism. Adherence to the intervention is >96%. No significant (≥grade II) toxicities are detected. Urinary urolithins (A, B, C, and D) and dimethyl ellagic acid (DMEA) quantified by Ultra high performance liquid chromatography tandem mass spectroscopy (UPLC/MS/MS) indicate a dose-dependent excretion yet heterogeneous patterns among men. Men in the BRB confection groups have greater urinary excretion of the microbial urinary metabolites urolithin A and DMEA, suggesting that this food matrix provides greater colonic microflora exposure. Conclusion: Fully characterized BRB confections and nectar are ideal for food-based large phase III human clinical studies. BRB products provide a bioavailable source of BRB phytochemicals, however large inter individual variation in polyphenol metabolism suggests that host genetics, microflora, and other factors are critical to understanding bioactivity and metabolism.

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Characterization of the Functional Changes in Mouse Gut Microbiome Associated with Increased Akkermansia muciniphila Population Modulated by Dietary Black Raspberries

Cited by 48 publications

References 58 publications

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Select Polyphenol-Rich Berry Consumption to Defer or Deter Diabetes and Diabetes-Related Complications

Dose‐Dependent Increases in Ellagitannin Metabolites as Biomarkers of Intake in Humans Consuming Standardized Black Raspberry Food Products Designed for Clinical Trials

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