Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Li, Yiyi; Yan, Hongmei; Zhang, Yao-Wei; Li, Qingping; Yu, Lu; Li, Qianyu; Liu, Cuiting; Xie, Yuwen; Chen, Keli; Ye, Feng; Wang, Kai; Chen, Longhua; Ding, Yi

doi:10.3389/fcimb.2020.541178

Cited by 60 publications

(67 citation statements)

References 51 publications

(54 reference statements)

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“…The applied 16S rRNA gene amplicon sequencing methods does, however, not make it possible to investigate this finding at a deeper taxonomic resolution. We observed that HFD mice have increased Dubosiella , which has been previously described in dysbiosis conditions such as GDM and obesity ( Bai et al, 2019 ; Li et al, 2020 ; Sheng et al, 2020 ; Qiu and Macchietto, 2021 ; Yi et al, 2021 ). Positive correlations of Dubosiella with FBG, FINS, and TC demonstrated that these bacteria may promote the glycolipid metabolism disorders, which could be reversed by ITF treatment.…”

Section: Discussionsupporting

confidence: 69%

The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation

Miao

Wang

et al. 2022

Front. Microbiol.

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BackgroundInulin-type fructans (ITF) have been used as prebiotics to alleviate glucose and lipid metabolism disorders. However, few studies evaluated the microbial mechanism of ITF in improving maternal metabolic status during pregnancy.MethodsC57BL/6J mice were fed a high-fat/sucrose diet (HFD) for 4 weeks before and throughout pregnancy to induce a model of gestational diabetes mellitus (GDM). Body weight, glycolipid metabolic parameters, and fecal short-chain fatty acids (SCFAs) were assessed in the experimental process. The effects of ITF on the fecal microbiota were analyzed by 16S rRNA gene amplicon sequencing.ResultsPregnant HFD-fed mice displayed significant insulin resistance and dyslipidemia. ITF (3.33 g/kg/day) treatment improved glucose and lipid metabolism disorder parameters in HFD-induced GDM mice and alleviated fat accumulation and glucose intolerance. The alpha diversity of the gut microbial community was increased in ITF mice, while the beta diversity returned to the level of normal chow diet (NCD) mice. Interestingly, Verrucomicrobia, Bifidobacterium, and Akkermansia were obviously enriched, while Dubosiella was obviously lessened after inulin treatment. Further analysis indicated that Dubosiella was positively correlated with markers of glycolipid metabolism disorders, whereas the ITF-supplemented diet partially reversed the changes.ConclusionOur results suggest that the ITF treatment may alleviate glucose and lipid metabolism disorders with the mediation of gut microbiota.

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Section: Discussionsupporting

confidence: 69%

The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation

Miao

Wang

et al. 2022

Front. Microbiol.

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“…Walking spurred an enrichment on some intestinal bacteria, such as Akkermansia , Bacteroides , Dubosiella , and Lactobacillus . Bacteroides shows an enrichment at the early stage after irradiation, implying that the bacteria might be a driver for radiation toxicity ( Wang et al, 2019 ; Li et al, 2020b ). Yet the effect of Dubosiella on enteritis is still controversial ( Sheng et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models

Wang

Jin

Dong

et al. 2021

Front. Cell Dev. Biol.

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Radiation-induced gastrointestinal (GI) tract toxicity halts radiotherapy and degrades the prognosis of cancer patients. Physical activity defined as “any bodily movement produced by skeletal muscle that requires energy expenditure” is a beneficial lifestyle modification for health. Here, we investigate whether walking, a low-intensity form of exercise, could alleviate intestinal radiation injury. Short-term (15 days) walking protected against radiation-induced GI tract toxicity in both male and female mice, as judged by longer colons, denser intestinal villi, more goblet cells, and lower expression of inflammation-related genes in the small intestines. High-throughput sequencing and untargeted metabolomics analysis showed that walking restructured the gut microbiota configuration, such as elevated Akkermansia muciniphila, and reprogramed the gut metabolome of irradiated mice. Deletion of gut flora erased the radioprotection of walking, and the abdomen local irradiated recipients who received fecal microbiome from donors with walking treatment exhibited milder intestinal toxicity. Oral gavage of A. muciniphila mitigated the radiation-induced GI tract injury. Importantly, walking did not change the tumor growth after radiotherapy. Together, our findings provide novel insights into walking and underpin that walking is a safe and effective form to protect against GI syndrome of patients with radiotherapy without financial burden in a preclinical setting.

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“…However, it has been found that severe postirradiation enteropathy is associated with low mucosal bacterial diversity ( Ferreira et al, 2019 ). In rodent studies, specific findings of microbiota changes in postirradiation fecal samples include increased abundance of the phylum Proteobacteria and family Lactobacillaceae and decreased abundance of families Lachnospiraceae , Ruminococcaceae , and Clostridiaceae , with some changes observed out to 10 months ( Lam et al, 2012 ; Goudarzi et al, 2016 ; Zhao et al, 2019 ; Li et al, 2020 ). In humans, one prospective study of nine gynecologic cancer patients found that Firmicutes and Fusobacterium phyla were significantly decreased in fecal samples pre- versus post-pelvic irradiation ( Nam et al, 2013 ).…”

Section: The Effects Of Radiation On the Microbiomementioning

confidence: 99%

Acute Radiation Syndrome and the Microbiome: Impact and Review

et al. 2021

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Study of the human microbiota has been a centuries-long endeavor, but since the inception of the National Institutes of Health (NIH) Human Microbiome Project in 2007, research has greatly expanded, including the space involving radiation injury. As acute radiation syndrome (ARS) is multisystemic, the microbiome niches across all areas of the body may be affected. This review highlights advances in radiation research examining the effect of irradiation on the microbiome and its potential use as a target for medical countermeasures or biodosimetry approaches, or as a medical countermeasure itself. The authors also address animal model considerations for designing studies, and the potential to use the microbiome as a biomarker to assess radiation exposure and predict outcome. Recent research has shown that the microbiome holds enormous potential for mitigation of radiation injury, in the context of both radiotherapy and radiological/nuclear public health emergencies. Gaps still exist, but the field is moving forward with much promise.

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Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis

Cited by 60 publications

References 51 publications

The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation

The Protective Effects of Inulin-Type Fructans Against High-Fat/Sucrose Diet-Induced Gestational Diabetes Mice in Association With Gut Microbiota Regulation

Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models

Acute Radiation Syndrome and the Microbiome: Impact and Review

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