Characterization of the Eimeria maxima sporozoite surface protein IMP1

Jenkins, Mark C.; Fetterer, Raymond H.; Miska, Katarzyna B.; Tuo, Wenbin; Kwok, O.; Dubey, J. P.

doi:10.1016/j.vetpar.2015.05.009

Cited by 15 publications

(6 citation statements)

References 22 publications

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“…The combination of rEt-ROPK-Eten5-A and other effective proteins (such as SO7 and ETRHO1) may be a new direction for the development of anticoccidial vaccines in the future. IMP1 was identified as an anticoccidial vaccine candidate, and it is localized in the sporozoite cell membrane [22]. However, the use of IMP1 in combination with a powerful immunological adjuvant (CD40L) did not improve the index of weight gain rate and oocyst decrease rate more than rEt-ROPK-Eten5-A [23].…”

Section: Discussionmentioning

confidence: 97%

“…Antigen refractile body protein (EtSO7) is located in sporozoite refractile bodies, and it provoked cellular and humoral immune responses to provide significant protection against cecal coccidiosis in immunized chickens [16]. Immune mapped protein 1 (IMP1) was recently identified as an anticoccidial vaccine candidate, and it is localized on the sporozoite cell membrane [22,23].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Rhoptry Protein as Candidate Vaccine against Eimeria tenella Infection

Song

Zhang

et al. 2020

Vaccines

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Eimeria tenella (E. tenella) is a highly pathogenic and prevalent species of Eimeria that infects chickens, and it causes a considerable disease burden worldwide. The secreted proteins and surface antigens of E. tenella at the sporozoite stage play an essential role in the host–parasite interaction, which involves attachment and invasion, and these interactions are considered vaccine candidates based on the strategy of cutting off the invasion pathway to interrupt infection. We selected two highly expressed surface antigens (SAGs; Et-SAG13 and Et-SAG) and two highly expressed secreted antigens (rhoptry kinases Eten5-A, Et-ROPK-Eten5-A and dense granule 12, Et-GRA12) at the sporozoite stage. Et-ROPK-Eten5-A and Et-GRA12 were two unexplored proteins. Et-ROPK-Eten5-A was an E. tenella-specific rhoptry (ROP) protein and distributed in the apical pole of sporozoites and merozoites. Et-GRA12 was scattered in granular form at the sporozoite stage. To evaluate the potential of rEt-ROPK-Eten5-A, rEt-GRA12, rEt-SAG13 and rEt-SAG proteins as a coccidiosis vaccine, the protective efficacy was examined based on survival rate, lesion score, body weight gain, relative body weight gain and oocyst output. The survival rate was significantly improved in rEt-ROPK-Eten5-A (100%) and rEt-GRA12 (100%) immune chickens compared to the challenged control group (40%). The average body weight gains of rEt-ROPK-Eten5-A, rEt-GRA12, rEt-SAG13 and rEt-SAG immunized chickens were significantly higher than those of unimmunized chickens. The mean lesion score and oocyst output of the rEt-ROPK-Eten5-A immunized chickens were significantly reduced compared to unimmunized challenged chickens. These results suggest that the rEt-ROPK-Eten5-A protein effectively triggered protection against E. tenella in chickens and provides a useful foundation for future work developing anticoccidial vaccines.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

A Novel Rhoptry Protein as Candidate Vaccine against Eimeria tenella Infection

Song

Zhang

et al. 2020

Vaccines

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“…Previous studies reported that monoclonal antibodies were able to detect proteins on the parasite surface, such as EtSAG1 and the micronemes of the sporozoites and merozoites [ 63 – 65 ]. Jenkins et al [ 66 ] showed the immune-mapped protein 1 could be detected in the sporozoites. Zhang et al [ 31 ] found EaMIC3 on the apical tip of E. acervulina sporozoites.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

et al. 2018

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BackgroundCoccidiosis is recognised as a major parasitic disease in chickens. Eimeria maxima is considered as a highly immunoprotective species within the Eimeria spp. family that infects chickens. In the present research, the surface antigen gene of E. maxima (EmSAG) was cloned, and the ability of EmSAG to stimulate protection against E. maxima was evaluated.MethodsProkaryotic and eukaryotic plasmids expressing EmSAG were constructed. The EmSAG transcription and expression in vivo was performed based on the RT-PCR and immunoblot analysis. The expression of EmSAG in sporozoites and merozoites was detected through immunofluorescence analyses. The immune protection was assessed based on challenge experiments. Flow cytometry assays were used to determine the T cell subpopulations. The serum antibody and cytokine levels were evaluated by ELISA.ResultsThe open reading frame (ORF) of EmSAG gene contained 645 bp encoding 214 amino acid residues. The immunoblot and RT-PCR analyses indicated that the EmSAG gene were transcribed and expressed in vivo. The EmSAG proteins were expressed in sporozoite and merozoite stages of E. maxima by the immunofluorescence assay. Challenge experiments showed that both pVAX1-SAG and the recombinant EmSAG (rEmSAG) proteins were successful in alleviating jejunal lesions, decreasing loss of body weight and the oocyst ratio. Additionally, these experiments possessed anticoccidial indices (ACI) of more than 170. Higher percentages of CD4+ and CD8+ T cells were detected in both EmSAG-inoculated birds than those of the negative control groups (P < 0.05). The EmSAG-specific antibody concentrations of both the rEmSAG and pVAX1-EmSAG groups were much higher than those of the negative controls (P < 0.05). Higher concentrations of IL-4, IFN-γ, TGF-β1 and IL-17 were observed more in both the rEmSAG protein and pVAX1-SAG inoculated groups than those of negative controls (P < 0.05).ConclusionsOur findings suggest that EmSAG is capable of eliciting a moderate immune protection and could be used as an effective vaccine candidate against E. maxima.

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“…Suo et al [ 68 ] proposed that the sporozoites are transported via the lymphatic vessels to the MLN and are then carried by lymph into the systemic circulation, after which the sporozoites continue their migration and finally enter the bile duct epithelial cells via the capillaries of intrahepatic bile ducts. AMA1 and IMP1 are expressed at high level in sporozoites and are located on the sporozoites surface [ 69 , 70 ]. The anti-r Et AMA1 polyclonal antibodies had a potent inhibitory effect on E. tenella sporozoite invasion, decreasing it by approximately 70% [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

et al. 2022

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Background Eimeria stiedae parasitizes the bile duct, causing hepatic coccidiosis in rabbits. Coccidiosis control using anticoccidials led to drug resistance and residues; therefore, vaccines are required as an alternative control strategy. Apical membrane antigen 1 (AMA1) and immune mapped protein 1 (IMP1) are surface-located proteins that might contribute to host cell invasion, having potential as candidate vaccine antigens. Methods Herein, we cloned and expressed the E. stiedae EsAMA1 and EsIMP1 genes. The reactogenicity of recombinant AMA1 (rEsAMA1) and IMP1 (rEsIMP1) proteins were investigated using immunoblotting. For the vaccination-infection trial, rabbits were vaccinated with rEsAMA1 and rEsIMP1 (both 100 μg/rabbit) twice at 2-week intervals. After vaccination, various serum cytokines were measured. The protective effects of rEsAMA1 and rEsIMP1 against E. stiedae infection were assessed using several indicators. Sera were collected weekly to detect the specific antibody levels. Results Both rEsAMA1 and rEsIMP1 showed strong reactogenicity. Rabbits vaccinated with rEsAMA1 and rEsIMP1 displayed significantly increased serum IL-2 (F(4, 25) = 9.53, P = 0.000), IL-4 (F(4, 25) = 7.81, P = 0.000), IL-17 (F(4, 25) = 8.55, P = 0.000), and IFN-γ (F(4, 25) = 6.89, P = 0.001) levels; in the rEsIMP1 group, serum TGF-β1 level was also elevated (F(4, 25) = 3.01, P = 0.037). After vaccination, the specific antibody levels increased and were maintained at a high level. The vaccination-infection trial showed that compared with the positive control groups, rabbits vaccinated with the recombinant proteins showed significantly reduced oocyst output (F(5, 54) = 187.87, P = 0.000), liver index (F(5, 54) = 37.52, P = 0.000), and feed conversion ratio; body weight gain was significantly improved (F(5, 54) = 28.82, P = 0.000). Conclusions rEsAMA1 and rEsIMP1 could induce cellular and humoral immunity, protecting against E. stiedae infection. Thus, rEsAMA1 and rEsIMP1 are potential vaccine candidates against E. stiedae. Graphic abstract

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Characterization of the Eimeria maxima sporozoite surface protein IMP1

Cited by 15 publications

References 22 publications

A Novel Rhoptry Protein as Candidate Vaccine against Eimeria tenella Infection

A Novel Rhoptry Protein as Candidate Vaccine against Eimeria tenella Infection

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

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