Characterization of the composition and antioxidant activity of plant extracts by HPLC with UV and amperometric detection

Semenistaya, E. N.; Ларионов, О. Г.

doi:10.1007/s11094-009-0167-5

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…Such compounds can also be delivered to the organism in the form of plant extracts as medicines, dietary supplements and cosmetics. However, due to the phenolic compounds differentiation with respect to structure, polarity and stability in the reaction medium, their antioxidant potential may vary, and it depends on the share of individual phenolic components in an extract, and on the way and conditions of the extraction process, as well as on the method of analysis (Rice-Evans et al, 1996, Semenistaya & Larionov, 2008. Precise determination of the composition of phenolic compounds in plant extracts and their antioxidant activity with respect to biological membranes is necessary for their rational and safe application to protect an organism against oxidation.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Cyboran¹,

Bonarska-Kujawa²,

Kapusta³

et al. 2011

Current Topics in Biophysics

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Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushesThe aim of the work was to determine the antioxidant potential of extracts from leaves of strawberry, blackcurrant and apple in relation to lipids contained in the erythrocyte membrane. The studies performed have shown that the substances used protect membrane lipids against oxidation, clearly reducing the level of free radicals in erythrocyte ghosts suspension. The antioxidant activity of the substances studied follows the sequence: strawberry leaves > apple leaves > blackcurrant leaves. The results of the research on the antioxidant activity when confronted with the contents of polyphenols in the extracts indicates that the antioxidant potentials of the extracts depend both on the quantity and kind of individual polyphenols; in particular, on the kind and quantity of quercetin derivatives that constitute over 60 % of all the phenolic compounds. Moreover, the high antioxidant activity of the extracts may be also due to other, nonphenolic substances that occur in leaves. The extracts exhibit very good properties as free radical scavenges, and can thus be used as cheap, easily available, natural antioxidants in the industries where natural antioxidants in the form of fruit extracts have been used for long.

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Section: Introductionmentioning

confidence: 99%

Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Cyboran¹,

Bonarska-Kujawa²,

Kapusta³

et al. 2011

Current Topics in Biophysics

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“…The existing methods for monitoring AO are based on the use of chromatographic [4][5][6][7][8], spectrophotometric [9,10] and chemiluminescent [11][12][13] methods, which are characterized as laborious, time-consuming and expensive. The research results are often incomparable, since they were obtained using various model systems [14].…”

Section: Introductionmentioning

confidence: 99%

Portable Amperometric Sensor for Measuring the Total Antioxidant Activity of Substances

2021

IJESRT

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Understanding the role of antioxidants as substances interrupting free radical reactions intensifies the search for new natural and synthetic substances with antioxidant properties. Along with this, intensive research is underway to create methods for assessing the antioxidant (AO) properties of various substances and devices for determining antioxidant activity (AOA). This article proposes an amperometric method and a sensor for measuring the total antioxidant activity of substances. The method is based on the process of oxygen electroreduction in the absence and presence of antioxidants of various origins in the solution. The measurement of the analytical signal before and after the introduction of the antioxidant substance into the electrochemical cell is carried out using a portable two-electrode sensor, "pencil" type, with a silver cathode, on which a potential of -500 mV is applied from a DC source (batteries). As an antioxidant standard, a 1% solution of ascorbic acid in a phosphate buffer with a pH of 6.86 was conventionally adopted. The sensor is connected to the measuring and driving device through a microcircuit - an operational amplifier. The measured signal in arbitrary units is displayed on the liquid crystal screen of the setting and measuring device and characterizes the maximum depolarization current of the indicator electrode. Using the resistors of the measuring device, a range of 0 - 100 units is set, for the analyte in the absence of an antioxidant and in the presence of a standard - 1% ascorbic acid solution, conventionally taken as 100%, respectively. Thus, the antioxidant activity of substances can be measured as a percentage relative to ascorbic acid (or other antioxidant). The results of determining the total antioxidant activity of some drinks and aqueous-alcoholic extracts of medicinal plants are presented.

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Uv-Spectrometry and the Lipid Composition of the Water-Propyleneglycol Extracts From Several Herbs

Олегович¹,

Николаевна²,

Анатольевич³

et al. 2020

JCPRM

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The physico-chemical properties by means of UV-spectroscopy and the lipid composition of extracts from Salvia officinalis L. leaves, the Calendula officinalis L and Matricaria chamomilla L. flowers; Hippophoe rhamnoides L fruits were studied. It is shown that extracts characterized the low shares of phospholipids and a trace content of sterols in the total lipid composition. Extracts studied are in the following sequence in accordance with decreasing the phospholipid share in the total lipid composition: Salvia officinalis L. leaves > Calendula officinalis L flowers = Hippophoe rhamnoides L fruits > Matricaria chamomilla L. flowers. It is shown that the quantitative ratio of phospholipid fractions is determined by the nature of the plant. Thus, the highest relative content of the phospholipid lysoforms in extracts was found in Calendula officinalis L flowers and Hippophoe rhamnoides L fruits, the highest share of sphingolipids was revealed in phospholipids of Calendula officinalis L and Matricaria chamomilla L flower extracts, and the phospholipids of Hippophoe rhamnoides L fruit extracts contained the highest amount of phosphatidylcholine. The high content of the more easily oxidizable fractions in the phospholipid composition especially for Salvia officinalis L. leaves are revealed. Using UV-spectrometry of extracts and mathematical analysis of these data the presence of flavonoids with different chemical structure is confirmed.

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Characterization of the composition and antioxidant activity of plant extracts by HPLC with UV and amperometric detection

Cited by 4 publications

References 8 publications

Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Portable Amperometric Sensor for Measuring the Total Antioxidant Activity of Substances

Uv-Spectrometry and the Lipid Composition of the Water-Propyleneglycol Extracts From Several Herbs

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