Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Cyboran, Sylwia; Bonarska-Kujawa, Dorota; Kapusta, Ireneusz; Oszmiański, Jan; Kleszczyńska, Halina

doi:10.2478/v10214-011-0003-4

Cited by 14 publications

(15 citation statements)

References 28 publications

(29 reference statements)

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“…The percent content of polyphenols in the extracts was determined with the liquid chromatography (HPLC) method described by Oszmiański et al [19]. Phenolic compounds were identified with the HPLC/DAD method described by Oszmiański et al [20] and the method of UPLC/ESI/MS analysis described by Cyboran et al [3]. Polyphenols were isolated from leaves by extraction with water containing 200 ppm of SO 2 , the ratio of solvent to leaves being 3:1.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, the protection of biological membranes, which are the main site of attack by free radicals, is directly due to the presence of polyphenols in the membrane and indirectly due to scavenging of free radicals in the medium. It has been shown that extracts from leaves of strawberry, blackcurrant and apple have very good antioxidant properties with respect to biological membranes, the strawberry extract exhibiting the highest activity, comparable to that of Trolox ® [3]. Plant extracts as substances of high biological activity have long been used in prevention and treatment, though the mechanism of their action at the cell and molecular level is not yet fully explained.…”

Section: Introductionmentioning

confidence: 99%

“…The subject of the present study is extracts from leaves of blackcurrant, strawberry and apple, which exhibit a series of positive actions with respect to biological systems. Extracts from currant leaves, aside from phenolic compounds that mainly belong to quercetin and kaempferol derivatives [2,3], also contain large amounts of prodelphinidins, and can thus be used for treating osteoarthritis [4]. Declume [5] showed that a water-alcohol extract from currant leaves has an antiinflammatory effect, and the tannins it contains are a competitive inhibitor of cAK with respect to both ATP and synthetic peptide substrate [6].…”

Section: Introductionmentioning

confidence: 99%

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Interaction between plant polyphenols and the erythrocyte membrane

Cyboran

Oszmiański

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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The purpose of these studies was to determine the effect of polyphenols contained in extracts from apple, strawberry and blackcurrant on the properties of the erythrocyte membrane, treated as a model of the biological membrane. To this end, the effect of the substances used on hemolysis, osmotic resistance and shape of erythrocytes, and on packing order in the hydrophilic region of the erythrocyte membrane was studied. The investigation was performed with spectrophotometric and fluorimetric methods, and using the optical microscope. The hemolytic studies have shown that the extracts do not induce hemolysis at the concentrations used. The results obtained from the spectrophotometric measurements of osmotic resistance of erythrocytes showed that the polyphenols contained in the extracts cause an increase in the resistance, rendering them less prone to hemolysis in hypotonic solutions of sodium chloride. The fluorimetric studies indicate that the used substances cause a decrease of packing order in the hydrophilic area of membrane lipids. The observations of erythrocyte shapes in a biological optical microscope have shown that, as a result of the substances’ action, the erythrocytes become mostly echinocytes, which means that the polyphenols of the extracts localize in the outer lipid monolayer of the erythrocyte membrane. The results obtained indicate that, in the concentration range used, the plant extracts are incorporated into the hydrophilic area of the membrane, modifying its properties.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction between plant polyphenols and the erythrocyte membrane

Cyboran

Oszmiański

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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“…The biological properties of CGA are primarily attributed to its capacity to donate hydrogen atoms of the phenolic ring to free radicals, thus inhibiting oxidation process [7]. Research confirms that CGA, among the polyphenol compounds that occur in plants, is an important natural antioxidant [5,6,[8][9][10][11][12]. The polyphenols are scavengers of free radicals which are responsible for oxidizing biological structures, with resultant damage and pathological states of an organism.…”

Section: Introductionmentioning

confidence: 99%

“…Chlorogenic acid (CGA) is a phenolic compound found in various parts of the plant, in fruits, vegetables, and herbs. It is present in large quantities in coffee beans, dry plums, cocoa fruits, hawthorn, nettle, bilberries, sunflower seeds, honeysuckle, potato tubers, and apples [1][2][3][4][5][6]. The biological properties of CGA are primarily attributed to its capacity to donate hydrogen atoms of the phenolic ring to free radicals, thus inhibiting oxidation process [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of chlorogenic acid on the phase transition in phospholipid and phospholipid/cholesterol membranes

Pruchnik

Bonarska-Kujawa

Kleszczyńska

2014

J Therm Anal Calorim

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Chlorogenic acid (CGA) is present in many plants, especially in green coffee, dry plums, and bilberries. It is an important bioactive polyphenol. Studies showed that CGA has an antioxidative, bacteriostatic, anticancer, antiviral, and anti-inflammatory activity. Despite great interest in this compound, its interaction with the lipid model membrane has not yet been investigated. To better understand the relationship between the biological activity of CGA and its interaction with biological membranes, the thermotropic behavior of model lipid membranes was investigated. The effect of CGA on the model lipid membrane, specifically on the lipid bilayer phase transitions, was examined by the combined methods: differential scanning calorimetry and fluorescence spectroscopy. In particular, the degree of packing order of the hydrophilic phase of the lipid bilayer was determined using the fluorimetric method with Laurdan and Prodan probes, while the fluorescence anisotropy of the hydrophobic phase with the DPH and TMA-DPH probes. The results of the study show that CGA incorporates mainly into the hydrophilic part of membrane, changing the packing order of the polar heads of lipids. No significant changes were recorded in membrane fluidity of the hydrophobic membrane region, for the fluorescence anisotropy practically did not change. One can thus infer that CGA does not penetrate deep into the hydrophobic area of the membrane.

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Activity of Hawthorn Leaf and Bark Extracts in Relation to Biological Membrane

et al. 2013

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The aim of the study was to identify and determine the percent content of polyphenols in extracts from leaves and hawthorn bark, to examine the effect of the extracts on the properties of the biological membrane as well as to determine their antioxidant activity toward membrane lipids. In particular, a biophysical investigation was conducted on the effect of hawthorn extracts on the osmotic resistance and morphology of erythrocyte cells and on the packing of the heads of membrane lipids. Analysis of the polyphenol content of extracts used the HPLC method. Analysis of the polyphenol composition has shown a dominant share of procyanidins and epicatechin in both extracts. The research showed that the polyphenolic compounds contained in hawthorn extracts are incorporated mainly into the hydrophilic part of the erythrocyte membrane, inducing echinocyte shapes. They also diminish the packing order of the lipid polar heads of the membrane, as evidenced by the lowered generalized polarization values of Laurdan. The substances used induced increased osmotic pressure of erythrocytes, making them less sensitive to changes in osmotic pressure. The presence of the extract compounds in the outer hydrophilic part of the erythrocyte membrane, evidenced by examination of the shapes and packing in the hydrophilic part of membrane, indicates that the substances constitute a kind of barrier that protects the erythrocyte membrane against free radicals, while the membrane-bound extracts do not disturb the membrane structure and, thus, do not cause any side effects.

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Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

Cited by 14 publications

References 28 publications

Interaction between plant polyphenols and the erythrocyte membrane

Interaction between plant polyphenols and the erythrocyte membrane

Effect of chlorogenic acid on the phase transition in phospholipid and phospholipid/cholesterol membranes

Activity of Hawthorn Leaf and Bark Extracts in Relation to Biological Membrane

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