Characterization of the Cholesteric Phase of Filamentous Bacteriophage fd for Molecular Alignment

Insights into Conformation and Dynamics of Protein GB1 During Folding and Unfolding by NMR

“…This could imply that the relative orientations of the amide N -H vectors of T11, L12 and K13 at pH 3.0 are different from that at pH 5.5, while the relative orientations for the majority of amide N -Hs are unchanged. However, RDCs measured in a second medium (liquid crystalline phase of fd phage) 19 with an independent alignment tensor ( Figure 1(I)) demonstrate that this explanation is not correct. The RDCs measured in both media exhibit the same type of deviation and small experimental values for the identical three residues are observed.…”

Virus‐Based Chemical and Biological Sensing

“…There are two reasons for this:[23,24] First, phages can express foreign peptides or proteins on the surface through insertion of genes that encode the foreign peptides or proteins into phage DNA (Figure 1), and then serve as a platform to test the nucleation of nanocrystals. Second, similar to wild-type phage,[57,58] nanoparticle-coated phages have a uniform size and shape, and thus can self-assemble into a 3D free-standing material with different architectures (for example, through the formation of a lyotropic liquid crystal). [23,24,48] Peptides, which were identified by a combinatorial library screening (biopanning) and specific to inorganic materials such as ZnS or CdS, were displayed on the major coat of the M13 phage, which resulted in the site-specific nucleation of nanocrystals with controlled organization and orientation of the crystal along the length of the phage (Figure 4).…”

Self-assembly approaches to nanomaterial encapsulation in viral protein cages

“…A few reports on virus capsid patterning and even hierarchical forms of organization have already emerged. 73,75,85,86 "Bottom-up" fabrication of hierarchical structures…”