Characterization of the Calcium Overload in Cultured Neonatal Rat Cardiomyocytes Under Metabolic Inhibition

Barrigón, Santos; Ji, Xiangwu; Langer, G. A.

doi:10.1006/jmcc.1996.0123

Cited by 12 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The slope of the line, which marks the onset of Ca 2+ transients (an indicator of impulse conduction velocity), was changed in the I-zone ( Fig. 5B; dotted line) consistently with decreased cell-to-cell coupling and diminished cell excitability in an ischemic environment (4,23,31).…”

Section: Effects Of Local Injury and Border Zone Effectsmentioning

confidence: 90%

“…With the exception of the work by Kleber and colleagues (12,13,27) and the observation of spontaneous rotors in chick myocyte cultures (6), multicellular networks of cultured myocytes remain to be explored as a tool to address the origins of ischemia-reperfusion arrhythmias. At the same time, a multitude of studies (4,23,25,(36)(37)(38) have addressed the effects of ischemic conditions on primary cultures of neonatal myocytes and freshly isolated cells from adult hearts. In such preparations, a hypoxic/anoxic, hyperkalemic, or acidic environment is applied to the entire sample (either to a coverslip or vial), and a variety of biochemical indices such as lactate dehydrogenase release, lipid peroxidation, permeability to trypan blue, or changes in action potential properties are measured.…”

Section: Discussionmentioning

confidence: 99%

“…Altogether, these technical issues make currently available potentiometric dyes limited to short experiments in which special arrays of photo sensors are used (13,28). In contrast, calcium dyes are stable and relatively nontoxic, with high quantum yield, which allows for using them in a variety of fluorescence imaging systems (2,4,6,37).…”

Section: Discussionmentioning

confidence: 99%

“…Each spontaneous or stimulated action potential caused a contraction and was associated with a large increase in the cytosolic free Ca 2+ concentration ([Ca 2+ ] i ) recorded as a fluo 4 transient. Monitoring of [Ca 2+ ] i transients as the means to record cell beating rate, wave front, and velocity of impulse propagation was successfully used in previous studies (4,16,37) that employed neonatal cardiac cells.…”

Section: Cell Loading With Fluorescent Indicatormentioning

confidence: 99%

See 3 more Smart Citations

Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias

Arutunyan¹,

Webster

Swift³

et al. 2001

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

We developed a new experimental approach to study the effects of local injury in a multicellular preparation and tested the ability of the method to induce reperfusion arrhythmias in cardiomyocyte monolayers. A small region of injury was created using geometrically defined flows of control and ischemia-like solutions. Calcium transients were acquired simultaneously from injured, control, and border zone cells using fluo 4. Superfusion with the injury solution rapidly diminished the amplitude of calcium transients within the injury zone, followed by cessation of cell beating. Reperfusion caused an immediate tachyarrhythmic response in approximately 17% of experiments, with a wave front propagating from a single cell or small cell cluster within the former injury zone. Inclusion of a gap junction uncoupler (1 mM heptanol) in the injury solution narrowed the functional border and sharply increased the number of ectopic foci and the incidence of reperfusion arrhythmias. The model holds a potential to reveal both micro- and macroscopic features of propagation, conduction, and cell coupling in the normal and diseased myocardium and to serve as a new tool to test antiarrhythmic protocols in vitro.

show abstract

Section: Effects Of Local Injury and Border Zone Effectsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Cell Loading With Fluorescent Indicatormentioning

confidence: 99%

See 2 more Smart Citations

Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias

Arutunyan¹,

Webster

Swift³

et al. 2001

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…Therefore we used the closest available model of fetal myocardium at term : a primary culture of neonatal rat cardiomyocytes in which cells beat synchronously [13] and develop pacemaker activity [14]. Neonatal rat cardiomyocytes provide a unique in vitro model for studying the rhythm, intracellular calcium dynamics and cell-to-cell interactions of cardiomyocytes [15][16][17]. Cardiomyocytes can be grown as primary cultures of single cells, each with its own independent rate of contraction, or as a network of cells that beat synchronously.…”

Section: Introductionmentioning

confidence: 99%

The bile acid taurocholate impairs rat cardiomyocyte function: a proposed mechanism for intra-uterine fetal death in obstetric cholestasis

et al. 2001

View full text Add to dashboard Cite

Obstetric cholestasis is a liver disease of pregnancy that can be complicated by sudden, hitherto unexplained, intra-uterine fetal death. Because intra-uterine death occurs suddenly, and because fetal heart rate abnormalities have been reported in obstetric cholestasis, we hypothesized that intra-uterine death is caused by impaired fetal cardiomyocyte function, resulting in fetal cardiac arrest. Obstetric cholestasis is associated with raised levels of maternal and fetal serum bile acids, and we propose that these may alter cardiomyocyte function. It was not possible to investigate the effects of bile acids on the intact human fetal heart at a cellular level. Therefore we used the closest available model of fetal myocardium at term: a primary culture of neonatal rat cardiomyocytes in which cells beat synchronously and develop pacemaker activity. The effect of the primary bile acid taurocholate (0.3 mM and 3 mM) on cultures of single cardiomyocytes, each with its own independent rate of contraction, was a reversible decrease in the rate of contraction and in the proportion of beating cells (P < 0.001). Addition of taurocholate to a network of synchronously beating cells caused a similar decrease in the rate of contraction. Furthermore, the integrity of the network was destroyed, and cells ceased to beat synchronously. Taurocholate also resulted in altered calcium dynamics and loss of synchronous beating. These data suggest that raised levels of the bile acid taurocholate in the fetal serum in obstetric cholestasis may result in the development of a fetal dysrhythmia and in sudden intra-uterine death.

show abstract

In vitro Cultured Cardiomyocytes for Evaluating Cardiotoxicity

Liu¹,

Melchert²

2018

Comprehensive Toxicology

View full text Add to dashboard Cite

Characterization of the Calcium Overload in Cultured Neonatal Rat Cardiomyocytes Under Metabolic Inhibition

Cited by 12 publications

References 0 publications

Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias

Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias

The bile acid taurocholate impairs rat cardiomyocyte function: a proposed mechanism for intra-uterine fetal death in obstetric cholestasis

In vitro Cultured Cardiomyocytes for Evaluating Cardiotoxicity

Contact Info

Product

Resources

About