Characterization of the biological effect of Biodentine<sup>TM</sup> on primary dental pulp stem cells

Hasweh, Nadine; Awidi, Abdalla; Rajab, Lamis D.; Hiyasat, Ahmad; Jafar, Hanan; Islam, Najmul; Hasan, Maram; Abuarqoub, Duaa

doi:10.4103/ijdr.ijdr_28_18

Cited by 12 publications

(8 citation statements)

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Section: Discussionsupporting

confidence: 91%

“…These results for Biodentine, are consistent with previous studies wherein MTT assays showed similar proliferative activity and cytotoxicity in different cell lines (Abuarqoub et al, 2020 ; Attik et al, 2014 ). In contrast, some studies have also reported decreased cell viability when exposed to high concentrations of Biodentine (Hasweh et al, 2018 ; Z. Luo et al, 2014 ). It should be noted that the cell morphology remained spindle‐shaped throughout the present study in both cements, which is consistent with previous reports (T. Luo et al, 2018 ; Lv et al, 2017 ).…”

Section: Discussionmentioning

confidence: 96%

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Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Rebolledo

Alcántara-Dufeu

Machuca

et al. 2023

Clinical & Exp Dental Res

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Introduction An ideal filling material should hermetically seal the communication pathways between the canal system and surrounding tissues. Therefore, during the last few years, the development of obturation materials and techniques to create optimal conditions for the proper healing of apical tissues has been a focus of interest. The effects of calcium silicate‐based cements (CSCs) on periodontal ligament cells have been investigated, and promising results have been obtained. To date, there are no reports in the literature that have evaluated the biocompatibility of CSCs using a real‐time live cell system. Therefore, this study aimed to evaluate the real‐time biocompatibility of CSCs with human periodontal ligament cells (hPDLCs). Methodology hPDLC were cultured with testing media of endodontic cements for 5 days: TotalFill‐BC Sealer, BioRoot RCS, Tubli‐Seal, AH Plus, MTA ProRoot, Biodentine, and TotalFill‐BC RRM Fast Set Putty. Cell proliferation, viability, and morphology were quantified using real‐time live cell microscopy with the IncuCyte S3 system. Data were analyzed using the one‐way repeated measures (RM) analysis of variance multiple comparison test ( p < .05). Results Compared to the control group, cell proliferation in the presence of all cements was significantly affected at 24 h ( p < .05). ProRoot MTA and Biodentine lead to an increase in cell proliferation; there were no significant differences with the control group at 120 h. In contrast, Tubli‐Seal and TotalFill‐BC Sealer inhibited cell growth in real‐time and significantly increased cell death compared to all groups. hPDLC co‐cultured with sealer and repair cements showed a spindle‐shaped morphology except with cements Tubli‐Seal and TotalFill‐BC Sealer where smaller and rounder cells were obtained. Conclusions The biocompatibility of the endodontic repair cements performed better than the sealer cements, highlighting the cell proliferation of the ProRoot MTA and Biodentine in real‐time. However, the calcium silicate‐based TotalFill‐BC Sealer presented a high percentage of cell death throughout the experiment similar to that obtained.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Rebolledo

Alcántara-Dufeu

Machuca

et al. 2023

Clinical & Exp Dental Res

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“…A total of 46 hCSCs were used in our included studies: 28 commercially available hCSCs along with 18 different modifications of hCSCs. PRMTA (n = 40) and BD (n = 34) were the most frequently used cements amongst all of the studies [ 37 , 38 , 39 , 41 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , …”

Section: Resultsmentioning

confidence: 99%

“…Compared to the 24 h II technique (n = 28), the dried heat (DH) condition was used in only four studies before the application of hCSCs. However, all of the reported biocompatibility and regenerative outcomes were similar (NSD) to the NC group or significantly better than NC (SH) [ 55 , 56 , 75 , 76 ]. Out of the four studies that examined the DH technique, only one of them reported their exact environment and conditions—a 50 °C oven for 15 min [ 53 ]—but the remaining three studies did not specify their environments.…”

Section: Resultsmentioning

confidence: 99%

Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review

Yousefi-Koma,

Assadian,

Mohaghegh

et al. 2023

JFB

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Background: Regenerative dentistry is the operation of restoring dental, oral and maxillofacial tissues. Currently, there are no guidelines for the ideal cement/material in regenerative endodontic treatments (RET). Hydraulic calcium silicate-based cements (hCSCs) are currently the material of choice for RET. Objectives: This systematic review was conducted to gather all of the different direct and indirect approaches of using hCSCs in RET in vitro and in vivo, and to ascertain if there are any superiorities to indirect approaches. Methods and Materials: This systematic review was conducted according to the 2020 PRISMA guidelines. The study question according to the PICO format was as follows: Comparison of the biological behavior (O) of stem cells (P) exposed to hCSCs through direct and indirect methods (I) with untreated stem cells (C). An electronic search was executed in Scopus, Google Scholar, and PubMed. Results: A total of 78 studies were included. Studies were published between 2010 and 2022. Twenty-eight commercially available and eighteen modified hCSCs were used. Seven exposure methods (four direct and three indirect contacts) were assessed. ProRoot MTA and Biodentine were the most used hCSCs and had the most desirable results. hCSCs were either freshly mixed or set before application. Most studies allowed hCSCs to set in incubation for 24 h before application, which resulted in the most desirable biological outcomes. Freshly mixed hCSCs had the worst outcomes. Indirect methods had significantly better viability/proliferation and odonto-/osteogenesis outcomes. Conclusion: Biodentine and ProRoot MTA used in indirect exposure methods result in desirable biological outcomes.

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“…Biodentine at low concentrations (2.00, 0.20 and 0.02 mg/ml) stimulates proliferation, viability and migration of SHEDs, whereas high concentrations of Biodentine (20 mg/ml) exhibit slight cytotoxicity to SHEDs. Changes in the concentration of Biodentine have no impact on the adhesion ability of SHEDs ( 128 ). Similarly, Biodentine at higher dilutions (1:16 and 1:32) is more effective in promoting proliferation, odontogenic differentiation and biomineralization of SHEDs, which may be because Sr and Si are gradually released from Biodentine as the concentration of Biodentine decreases over time ( 129 ).…”

Section: Stem Cellsmentioning

confidence: 99%

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

Song

Tang

et al. 2021

Int J Mol Med

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Characterization of the biological effect of Biodentine^TM on primary dental pulp stem cells

Cited by 12 publications

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Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

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Characterization of the biological effect of BiodentineTM on primary dental pulp stem cells

Cited by 12 publications

References 0 publications

Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Real‐time evaluation of the biocompatibility of calcium silicate‐based endodontic cements: An in vitro study

Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

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Characterization of the biological effect of Biodentine^TM on primary dental pulp stem cells