Characterization of the Betaine Lipids, Diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) and Diaclyglycerylhydroxymethyl-N,N,N-trimethyl-β-alanine (DGTA), in Brown- and Green-Pigmented Raphidophytes

Leblond, Jeffrey D.; Dahmen, Aaron; Dodson, V. Joshua; Dahmen, Jeremy L.

doi:10.1127/1864-1318/2013/0130

Cited by 7 publications

(5 citation statements)

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“…The presence of betaine lipids (BL) among eukaryotic phytoplankton (Dembitsky, ) represents additional lipid classes specifically adapted to replace PL in low‐P environments that cannot be found in higher plants. This richer lipid diversity is becoming better elucidated (Armada et al ., ; Banskota et al ., ; Leblond et al ., ; Anesi & Guella, ; Cañavate et al ., ) and suggests that the microalgal lipid profile could be even more plastic than that of higher plants under the influence of P nutrition. However, significant research advances are still needed to determine the extent of microalgal differentiation compared with higher plants in the lipid response to P availability (Boudière et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

2016

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The response of marine microalgal lipids to phosphorus is of central importance in phytoplankton ecology but remains poorly understood. We determined how taxonomically diverse microalgal species remodelled their lipid class profile in response to phosphorus availability and whether these changes coincided with those already known to occur in land plants and in the limited number of phytoplankton species for which data are available. The complete lipid class profile and specific lipid ratios influenced by phosphorus availability were quantified in two green microalgae and seven Chromalveolates exposed to phosphorus repletion, deprivation and replenishment. Lipid class cell quota changes in the two green microalgae resembled the currently described pattern of betaine lipids substituting for phospholipids under phosphorus depletion, whereas only two of the studied Chromalveolates showed this pattern. Sulpholipids counterbalanced phosphatidylglycerol only in Picochlorum atomus. In all other species, both lipids decreased simultaneously under phosphorus deprivation, although sulpholipids declined more slowly. Phosphorus deprivation always induced a decrease in digalactosyl-diacylglycerol. However, the ratio of digalactosyl-diacylglycerol to total phospholipids increased in eight species and remained unchanged in Isochrysis galbana. Marine phytoplankton seems to have evolved a diversified mechanism for remodelling its lipid class profile under the influence of phosphorus, with cryptophytes and particularly haptophytes exhibiting previously unobserved lipid responses to phosphorus.

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Section: Introductionmentioning

confidence: 99%

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

2016

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“…In this case, only one pair of ions from the losses of fatty acid was observed, corresponding to the loss of intact neutral fatty acids ([M + H‐R 1/2 COOH] + ) and of the acyl groups ([M + H‐R 1/2 C = O] + ) for each of the substituted fatty terminals. Moreover, ions [C 10 H 22 O 5 N] + at m/z 236.15 (removal of both fatty acids from the intact molecule) and [C 7 H 14 O 2 N] + at m/z 144.10 (loss of glycerol and both fatty acids) were considered as the characteristic ions of DGTS, which was consistent with other studies . Here, m/z 144.10 represents the polar head group.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, the diversity of forms of DGTS and DGTA in chlorarachniophyte algae was examined using positive‐ion electrospray ionization/mass spectrometry (ESI‐MS) and electrospray ionization/tandem mass spectrometry (ESI‐MS/MS), which was the first application of this type of MS technology for the characterization of betaine lipids from a class of algae. Several studies showed that DGTS had a base peak with characteristic loss of m/z 87 [‐CH 2 ‐CH 2 ‐N + (CH 3 ) 3 ], whereas the base peak of DGTA was its molecular weight . Based on this result, the characteristic loss of m/z 87 ([M + Na‐87] + ) can be used to differentiate between the structures of DGTS and DGTA.…”

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confidence: 99%

Simultaneous structural identification of diacylglyceryl‐N‐trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl‐N,N,N‐trimethyl‐β‐alanine (DGTA) in microalgae using dual Li⁺/H⁺ adduct ion mode by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Lou

Mu³

et al. 2017

Rapid Comm Mass Spectrometry

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“…However, their concentrations in marine environments range from picomolar to hundreds of nanomolar (Diaz et al, 2016; Paul Hansard et al, 2010; Roe et al, 2016; Rose et al, 2008; Rusak et al, 2011). As discussed previously, PhytoPs are very likely produced by diatoms for which only certain glycerolipids (phosphatidylcholine and diacylglyceryl-hydroxymethyl-N,N,N-trimethyl-β-alanine) present in intracellular endomembranes, which contained sufficient amounts of its PUFA precursor (ALA) to justify a role for the production of PhytoPs (Leblond et al, 2013; Lupette et al, 2018). Even if higher concentrations of extracellular ROS are often measured in high-productivity waters and shallow coastal environments (Diaz et al, 2016; Paul Hansard et al, 2010; Roe et al, 2016; Rose et al, 2008; Rusak et al, 2011), their concentrations might be not sufficient to trigger all the intracellular peroxidation in diatoms providing the oxylipins amounts measured in the present study.…”

Section: Discussionmentioning

confidence: 99%

Non-enzymatic oxylipin production in a mudflat microphytobenthic biofilm: evidence of a diatom response to light

Doose,

Oger,

Mas-Normand

et al. 2024

Preprint

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The microphytobenthos (MPB) is a diatom dominated microbial community of primary producers inhabiting the mudflat sediments. In one hand, the benthic diatoms display photo-protective strategies to face extreme light variations susceptible to generate cellular oxidative stress. In the other hand, oxidative stress induces the production of reactive oxygen species (ROS) that generate oxylipins, oxygenated metabolites of polyunsaturated fatty acids (PUFAs), which are among the known chemical mediators in diatoms. However, non-enzymatically generated oxylipins known as isoprostanoids or isofuranoids are poorly studied in diatoms. To better understand the roles of the latter in migrational MPB light response, we investigated the effect of different light irradiances corresponding to dark (D), low light (LL, 50 and 100 umol. photons. m-2. s-1PAR), medium light (ML, 250 umol. photons. m-2. s-1 PAR), high light (HL, 500, 750 and 1000 umol. photons. m-2. s-1PAR), on the isoprostanoids production by the biofilm's organisms. The PUFAs precursors of the varying oxylipins evidenced a diatoms response to light irradiance. Under 1000 PAR condition, the total amount of isoprotanoids increased, indicating an oxidative stress response. Isoprostanes (IsoPs) and prostaglandins (PGs) characterized the HL conditions and evidenced lipid peroxidation probably linked to the higher generation of ROS by the photosynthesis. On the contrary, the phytoprostanes (PhytoPs) characterized the LL and ML where the de-epoxidation state was low and ROS scavengers probably not overwhelmed. This first investigation of non-enzymatic oxylipin production by a microphytobenthic biofilm under different light irradiances highlighted the interest to explore their potential signaling roles related to MPB light responses.

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Characterization of the Betaine Lipids, Diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) and Diaclyglycerylhydroxymethyl-N,N,N-trimethyl-β-alanine (DGTA), in Brown- and Green-Pigmented Raphidophytes

Cited by 7 publications

References 0 publications

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

Non-enzymatic oxylipin production in a mudflat microphytobenthic biofilm: evidence of a diatom response to light

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