Characterization of the bacterial microbiota of <i>Biomphalaria glabrata (Say, 1818) (Mollusca: Gastropoda)</i>
 from Brazil

Silva, T.M.; Melo, Elverson Soares de; Lópes, Ana Catarina de Souza; Veras, Dyana Leal; Duarte, Cybelle Rodrigues; Alves, Luiz Cláudio Pinto de Sá; Brayner, Fábio André

doi:10.1111/lam.12068

Cited by 23 publications

(19 citation statements)

References 28 publications

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“…A few studies have characterized the cultivable flora of B. glabrata and have identified Aeromonadaceae, Enterobacteriaceae, Moraxellaceae, and Pseudomonadaceae as being the most prevalent bacterial families in this species (Ducklow et al, 1979(Ducklow et al, , 1981Silva et al, 2013). The dominant families described in the previous studies were also represented in our dataset.…”

Section: Discussionmentioning

confidence: 99%

Schistosomiasis Vector Snails and Their Microbiota Display a Phylosymbiosis Pattern

et al. 2020

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Planorbidae snails are the intermediate host for the trematode parasite of the Schistosoma genus, which is responsible for schistosomiasis, a disease that affects both humans and cattle. The microbiota for Schistosoma has already been described as having an effect on host/parasite interactions, specifically through immunological interactions. Here, we sought to characterize the microbiota composition of seven Planorbidae species and strains. Individual snail microbiota was determined using 16S ribosomal DNA amplicon sequencing. The bacterial composition was highly specific to the host strain with limited interindividual variation. In addition, it displayed complete congruence with host phylogeny, revealing a phylosymbiosis pattern. These results were confirmed in a common garden, suggesting that the host highly constrains microbial composition. This study presents the first comparison of bacterial communities between several intermediate snail hosts of Schistosoma parasites, paving the way for further studies on the understanding of this tripartite interaction.

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Section: Discussionmentioning

confidence: 99%

Schistosomiasis Vector Snails and Their Microbiota Display a Phylosymbiosis Pattern

et al. 2020

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“…This is the first Illumina study performed on samples of both field-derived vector snails and their corresponding schistosome parasites, adding a unique perspective to our understanding of schistosome transmission “in the wild” in endemic regions. This approach also serves to remind us that the snails targeted for infection by schistosome miracidia in the field are best considered as holobionts with potentially complex sets of symbiotic associates [ 61 , 62 ]. Finally, we note that this study will add to the literature a considerable amount of new data for B .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

Buddenborg

Zhang

et al. 2017

PLoS Negl Trop Dis

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BackgroundBiomphalaria pfeifferi is highly compatible with the widespread human-infecting blood fluke Schistosoma mansoni and transmits more cases of this parasite to people than any other snail species. For these reasons, B. pfeifferi is the world’s most important vector snail for S. mansoni, yet we know relatively little at the molecular level regarding the interactions between B. pfeifferi and S. mansoni from early-stage sporocyst transformation to the development of cercariae.Methodology/Principal findingsWe sought to capture a portrait of the response of B. pfeifferi to S. mansoni as it occurs in nature by undertaking Illumina dual RNA-Seq on uninfected control B. pfeifferi and three intramolluscan developmental stages (1- and 3-days post infection and patent, cercariae-producing infections) using field-derived west Kenyan specimens. A high-quality, well-annotated de novo B. pfeifferi transcriptome was assembled from over a half billion non-S. mansoni paired-end reads. Reads associated with potential symbionts were noted. Some infected snails yielded fewer normalized S. mansoni reads and showed different patterns of transcriptional response than others, an indication that the ability of field-derived snails to support and respond to infection is variable. Alterations in transcripts associated with reproduction were noted, including for the oviposition-related hormone ovipostatin and enzymes involved in metabolism of bioactive amines like dopamine or serotonin. Shedding snails exhibited responses consistent with the need for tissue repair. Both generalized stress and immune factors immune factors (VIgLs, PGRPs, BGBPs, complement C1q-like, chitinases) exhibited complex transcriptional responses in this compatible host-parasite system.SignificanceThis study provides for the first time a large sequence data set to help in interpreting the important vector role of the neglected snail B. pfeifferi in transmission of S. mansoni, including with an emphasis on more natural, field-derived specimens. We have identified B. pfeifferi targets particularly responsive during infection that enable further dissection of the functional role of these candidate molecules.

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“…Moreover, all bacterial communities characterized and cultivated from B . glabrata were gram-negatives [91], and everything that came in contact with the mollusk (e.g., water, food) tested negative for Paenibacillus , strongly suggesting contamination of animals collected in the field and horizontal transmission between laboratory snails. Indeed as WHO collaborating center we recovered in the field and reared in our laboratory a great number of snail strains from different localities (mainly South America and Africa).…”

Section: Discussionmentioning

confidence: 99%

A Novel Bacterial Pathogen of Biomphalaria glabrata: A Potential Weapon for Schistosomiasis Control?

et al. 2015

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BackgroundSchistosomiasis is the second-most widespread tropical parasitic disease after malaria. Various research strategies and treatment programs for achieving the objective of eradicating schistosomiasis within a decade have been recommended and supported by the World Health Organization. One of these approaches is based on the control of snail vectors in endemic areas. Previous field studies have shown that competitor or predator introduction can reduce snail numbers, but no systematic investigation has ever been conducted to identify snail microbial pathogens and evaluate their molluscicidal effects.Methodology/Principal findingsIn populations of Biomphalaria glabrata snails experiencing high mortalities, white nodules were visible on snail bodies. Infectious agents were isolated from such nodules. Only one type of bacteria, identified as a new species of Paenibacillus named Candidatus Paenibacillus glabratella, was found, and was shown to be closely related to P. alvei through 16S and Rpob DNA analysis. Histopathological examination showed extensive bacterial infiltration leading to overall tissue disorganization. Exposure of healthy snails to Paenibacillus-infected snails caused massive mortality. Moreover, eggs laid by infected snails were also infected, decreasing hatching but without apparent effects on spawning. Embryonic lethality was correlated with the presence of pathogenic bacteria in eggs.Conclusions/SignificanceThis is the first account of a novel Paenibacillus strain, Ca. Paenibacillus glabratella, as a snail microbial pathogen. Since this strain affects both adult and embryonic stages and causes significant mortality, it may hold promise as a biocontrol agent to limit schistosomiasis transmission in the field.

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Characterization of the bacterial microbiota of Biomphalaria glabrata (Say, 1818) (Mollusca: Gastropoda) from Brazil

Cited by 23 publications

References 28 publications

Schistosomiasis Vector Snails and Their Microbiota Display a Phylosymbiosis Pattern

Schistosomiasis Vector Snails and Their Microbiota Display a Phylosymbiosis Pattern

Transcriptomic responses of Biomphalaria pfeifferi to Schistosoma mansoni: Investigation of a neglected African snail that supports more S. mansoni transmission than any other snail species

A Novel Bacterial Pathogen of Biomphalaria glabrata: A Potential Weapon for Schistosomiasis Control?

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