Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Rashan, Luay; Franke, Katrin; Khine, Myint Myint; Kelter, Gerhard; Fiebig, Heinz H.; Neumann, Joachim; Wessjohann, Ludger A.

doi:10.1016/j.jep.2011.01.038

Cited by 58 publications

(35 citation statements)

References 37 publications

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“…Furthermore, interestingly, malignant cells are generally more susceptible to the effects of CTSs than normal cells. This mediation may be caused by the altered density of an enzyme in the plasma cell membrane and by changes in isoenzyme expression in malignant cells (Malikova et al, 2008;Rashan et al, 2011). In line with this observation, the present study's data also showed that the U. maritima composition caused greatly significantly reduced viability coupled with morphological alteration of malignant neuroblastoma SH-SY5Y cells (Fig.…”

Section: Discussionsupporting

confidence: 87%

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

Elghuol¹,

Khalil

Zain

et al. 2016

American Journal of Applied Sciences

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Natural products have played a significant role in conventional treatment and in the development of new drugs for a wide range of illnesses, including malignant disease. Urginea maritima is classified as an herb is endemic to the Mediterranean area. This herb was renowned in traditional ethnopharmacological applications. Notwithstanding, there is a lack of evidence pertaining to the characterization of the Urginea maritima constituents contained within a water-based extract and their bioactivity in human neurological disorders. Therefore, we sought to investigate the composition and to evaluate its antitumour capability against the human malignant neuroblastoma cells. Through the utilization of several in vitro techniques, the present work illustrates that cardiotonic steroids are the main constituents of Urginea maritima aqueous extract and grouping of polyphenolic compounds are present. This result was based on interpretation of the mass spectrum obtained via electrospray ionization timeof-flight tandem mass spectrometry. The current study finding confirms that the constituents of the extract trigger a series of toxic reactions. In terms of cell proliferation inhibition, malignant SH-SY5Y neuroblastoma cell proliferation was selectively inhibited in a time-dose dependent manner. As verified quantitatively using the MTS colorimetric assay, with less toxicity was observed toward neuron-model cells. Apoptosis was efficiently induced in SH-SY5Y neuroblastoma cells as evidenced by Fluorescent microscopy using PI staining and DNA-specific dye DAPI revealed nuclear fragmentation, which profounds the apoptosis mechanism. Our novel findings clearly emphasize that the principal constituents of this unusual natural product can exhibit dual effects on neuron cells. This preferential selectivity provides an interesting basis for widespread medical application and a promising therapeutic strategy against neurological diseases, specifically human malignant neuroblastoma disorders.

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Section: Discussionsupporting

confidence: 87%

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

Elghuol¹,

Khalil

Zain

et al. 2016

American Journal of Applied Sciences

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“…However, recently, pleiotropic anticancer activities of cardenolide glycosides have been discovered (Rashan et al, 2011;Li et al, 2012;Cerella et al, 2012;Xue et al, 2013). Currently, several species of Asclepias genus have been investigated as a source of cardenolide glycosides (Mijatovic et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative activity of cardenolide glycosides from Asclepias subulata

Rascón-Valenzuela

Velázquez

Garibay‐Escobar

et al. 2015

Journal of Ethnopharmacology

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“…While the impact of C3 glycosylation upon the anticancer activity of steroidal glycosides has been previously reported, 3a,18 the influence of C12 glycosylation upon bioactivity of this natural product class had not. Thus, the enzymatic synthesis of 10 was subsequently scaled to enable bioactivity assessment.…”

mentioning

confidence: 92%

Probing the Regiospecificity of Enzyme-Catalyzed Steroid Glycosylation

et al. 2012

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The potential of a uniquely permissive engineered glycosyltransferase (OleD ASP) as a catalyst for steroid glycosylation is highlighted. The ability of OleD ASP to glucosylate a range of cardenolides and bufadienolides was assessed using a rapid LC-UV/MS-SPE-NMR analytical platform. While a bias toward OleD-catalyzed C3 mono-glucosylation was observed, subtle alterations of the steroidal architecture in some cases, invoked diglucosylation or, in one case (digoxigenin), C12 glucosylation. This latter case represents the first, and highly efficient, synthesis of digoxigenin 12-O-β-D-glucoside.

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Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Cited by 58 publications

References 37 publications

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

Antiproliferative activity of cardenolide glycosides from Asclepias subulata

Probing the Regiospecificity of Enzyme-Catalyzed Steroid Glycosylation

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