Antiproliferative activity of cardenolide glycosides from Asclepias subulata

Rascón-Valenzuela, Luisa Alondra; Velázquez, Carlos; Garibay‐Escobar, Adriana; Medina-Juárez, Luis Ángel; Vilegas, Wagner; Robles‐Zepeda, Ramón Enrique

doi:10.1016/j.jep.2015.05.057

Cited by 17 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…halimifolium , a qualitative phytochemical screening was conducted. We used the next specific test: saponins (Rosenthaler test, foam test), cumarines (KOH test), glycosides (Keller-Kiliani test), sterols and terpenes (Liebermann-Buchnard), alkaloids (Wagner, Meyer test), tannins (gelatin test), flavonoids (NaOH 10% test), quinones (chloroform test), and phenol compounds (ferric chloride test) (Harborne, 1973, Gupta et al, 2013, Banu and Cathrine, 2015, Rascon-Valenzuela et al, 2015, Prabhavathi et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844)

Meneses-Sagrero

Navarro-Navarro

Ruíz-Bustos

et al. 2017

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Cancer is the major cause of death in the world, representing a significant public health problem. Plants have been shown as a great source of secondary metabolites with anticancer activity. The aim of this work was evaluated the antiproliferative activity of the methanolic extracts, chemical fractions and the compound spinasterol isolated of medicinal plant . The methanolic extracts of stem, leaf and stem/leaf was obtained by maceration. The methanolic extract of stem was purified by successive extractions with solvents as-hexane, ethyl acetate and ethanol. The -hexane fraction was separated by column chromatographic and monitored by thin layer chromatographic. The compound spinasterol was characterized byH NMR, C NMR and Mass Spectrometry. Methanolic extracts, chemical, chromatographic fractions and spinasterol was evaluated against RAW 264.7, M12.C3.F6,, LS-180, A549 and HeLa cancer cell lines by the standardized method MTT for determinate the antiproliferative activity. Methanolic extract of stem shown the better antiproliferative activity against the murine macrophage cancer cell line RAW 264.7. -Hexane chemical fraction shown antiproliferative activity against human alveolar cancer cell line A549 and RAW 264.7. Was isolated and characterized a compound by NMRH and C, revealing the presence of sterol spinasterol. Spinasterol shown have antiproliferative activity against cervical cancer cell line HeLa and RAW 264.7, that spinasterol can be a responsible compound of antiproliferative activity found in the methanolic extract of .

show abstract

Section: Methodsmentioning

confidence: 99%

Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844)

Meneses-Sagrero

Navarro-Navarro

Ruíz-Bustos

et al. 2017

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

show abstract

“…Compound 3 was identified as a known compound crotoxigenin 3- O -glucopyranoside based on the NMR data, mass spectral analysis, and previous published data ( Figure 4 ) [ 13 ]. It was isolated as a white crystalline solid (5 mg).…”

Section: Resultsmentioning

confidence: 99%

A Novel Cardenolide Glycoside Isolated from Xysmalobium undulatum Reduces Levels of the Alzheimer’s Disease-Associated β-Amyloid Peptides Aβ42 In Vitro

et al. 2021

View full text Add to dashboard Cite

Elevated levels of the amylo β-proteins (Aβ), particularly Aβ42, are associated with a high risk of Alzheimer’s disease (AD). The Aβ proteins are produced from cellular processing of the amyloid precursor proteins (APPs). To identify natural products that block the formation of Aβ-proteins from APPs, we previously screened a library of plant extracts and identified Xysmalobium undulaum (Apocynaceae) as a potential plant for further research. Here, we provide a report on the isolation and identification of the active principles from the plant species using a bioassay-guided fractionation. Fractions and resulting pure compounds from the purification process of the extract of X. undulatum were screened in vitro against APPs transfected HeLa cell lines. Three compounds, acetylated glycosydated crotoxogenin (1), xysmalogenin-3, β-d-glucopyranoside (2), and crotoxigenin 3-O-glucopyranoside (3), were subsequently isolated and their structures elucidated using NMR and mass spectrometry. Compound 1, a novel cardenolide, and 2 significantly decreased the Aβ42 levels in a dose-dependent manner while compound 3 was inactive. In silico investigations identified the AD’s β-secretase enzyme, BACE1, as a potential target for these compounds with the glycoside moiety being of significance in binding to the enzyme active site. Our study provides the first report of a novel cardenolide and the potential of cardenolides as chemical scaffolds for developing AD treatment drugs.

show abstract

“…In order to provide scientific validation of traditional medicinal use of A. subulata, prior study evaluated the antiproliferative activity of methanol extract of the plant and its solvent fractions (hexane, ethyl acetate, ethanol and residual) demonstrating that methanol extract of A. subulata and its polar solvent fractions have a strong antiproliferative activity in a number of human cancer cell lines (A549, PC-3, HeLa and LS 180) with IC 50 values in the range of 0.31-18 mg/mL. In addition, that study revealed that chromatographic fractions of methanol extract of the plant induced the cell death by apoptosis (Rascón-Valenzuela et al, 2015a).…”

Section: Introductionmentioning

confidence: 82%