Characterization of TEMINAL FLOWER1 homologs CmTFL1c gene from Chrysanthemum morifolium

Gao, Yaohui; Gao, Yu; Wu, Zhicheng; Bu, Xianglong; Fan, Min; Zhang, Qixiang

doi:10.1007/s11103-019-00838-6

Cited by 21 publications

(27 citation statements)

References 42 publications

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“…An Escherichia coli phosphomannose isomerase (PMI) marker gene and mannose selective agent were used in a mannose selection system for transformation in chrysanthemum. Finally, amplification of CmTFL1a from pEASY- CmTFL1a with CmTFL1a-pmi -F and CmTFL1a-pmi -R primers was accomplished, followed by digestion of pCAMBIA 1301- pmi vector with Xho I, leading towards assembling of CmTFL1a into the plasmid using Seamless Assembly [ 21 ]. The experiment was repeated three times to ensure the best possible results.…”

Section: Methodsmentioning

confidence: 99%

“…TFL1 plays a key role in suppressing flowering by antagonizing the function of the FT protein, which is responsible for the promotion of flowering [ 20 ]. Moreover, it helps in the formation of inflorescences, maintains vegetative growth and an indeterminate inflorescence [ 21 , 22 ]. Several genes homologous to TFL1 have been identified in many plants.…”

Section: Introductionmentioning

confidence: 99%

“…Several genes homologous to TFL1 have been identified in many plants. Some of the examples include Malus domestica [ 23 ], Chrysanthemum morifolium [ 21 ] and Chrysanthemum Seticuspe f. boreale [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…This work aims to enhance the number of putative regenerated shoots of Jinba by optimizing the protocol of Agrobacterium -mediated transformation, to maximize the feasibility of producing transgenic plants by standardizing media composition, and to examine the effect of CmTFL1a expression on the flowering of chrysanthemum plants by phenotypic analysis. CmTFL1a was isolated from C. morifolium Jinbudiao in our lab and its functional role in chrysanthemum was analyzed as mentioned in [ 21 ]. CmTFL1a was inserted into in vitro Jinba plants as a transgene to obtain transgenic lines.…”

Section: Introductionmentioning

confidence: 99%

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Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Haider

Gao

2020

Genes

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Chrysanthemum (Chrysanthemum x morifolium Ramat.) cultivar Jinba is a distinctive short-day chrysanthemum that can be exploited as a model organism for studying the molecular mechanism of flowering. The commercial value of Jinba can be increased in global flower markets by developing its proper regeneration and genetic transformation system. By addressing typical problems associated with Agrobacterium-mediated transformation in chrysanthemum, that is, low transformation efficiency and high cultivar specificity, we designed an efficient, stable transformation system. Here, we identify the features that significantly affect the genetic transformation of Jinba and standardize its transformation protocol by using CmTFL1a as a transgene. The appropriate concentrations of various antibiotics (kanamycin, meropenem and carbenicillin) and growth regulators (6-BA, 2,4-D and NAA) for the genetic transformation were determined to check their effects on in vitro plant regeneration from leaf segments of Jinba; thus, the transformation protocol was standardized through Agrobacterium tumefaciens (EHA105). In addition, the presence of the transgene and its stable expression in CmTFL1a transgenic plants were confirmed by polymerase chain reaction (PCR) analysis. The CmTFL1a transgene constitutively expressed in the transgenic plants was highly expressed in shoot apices as compared to stem and leaves. Overexpression of CmTFL1a led to a delay in transition to the reproductive phase and significantly affected plant morphology. This study will help to understand the biological phenomenon of TFL1 homolog in chrysanthemum. Moreover, our findings can explore innovative possibilities for genetic engineering and breeding of other chrysanthemum cultivars.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Haider

Gao

2020

Genes

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“…Hanano and Goto [52] found that the TFL1 protein is localized to the nucleus and cytoplasm. However, CmTFL1c from Chrysanthemum morifolium also belongs to the nucleus and plasma membrane [16]. In this study, BnA10.TFL1 protein was found to localize to the nucleus, cytoplasm, and plasma membrane ( Figure 6).…”

Section: Discussionmentioning

confidence: 52%

Characterization of the BnA10.tfl1 Gene Controls Determinate Inflorescence Trait in Brassica napus L.

Jia

Liu

et al. 2019

Agronomy

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Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene BnTFL1 (Terminal Flower 1) for control of the inflorescence trait on Brassica napus chromosome A10 (16,627–16,847 kb). However, little is known about the function of the BnTFL1 gene in B. napus. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and BnA10.TFL1 expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named BnA10.TFL1 gene from indeterminate and BnA10.tfl1 gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with Arabidopsis thaliana TFL1. To analyze the function of BnA10.TFL1, the BnA10.TFL1 gene was introduced into the determinate A. thaliana tfl1 mutant and B. napus 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of BnA10.TFL1 was increased in the indeterminate shoot apex. These results reveal that BnA10.tfl1 is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in B. napus.

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Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

et al. 2019

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Chrysanthemum (Chrysanthemum morifolium Ramat.) is a leading flower with applied value worldwide. Developing new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes, plant architectures, flowering times, postharvest quality, and biotic and abiotic stress tolerance in a time- and cost-efficient manner is the ultimate goal for breeders. Various breeding strategies have been employed to improve the aforementioned traits, ranging from conventional techniques, including crossbreeding and mutation breeding, to a series of molecular breeding methods, including transgenic technology, genome editing, and marker-assisted selection (MAS). In addition, the recent extensive advances in high-throughput technologies, especially genomics, transcriptomics, proteomics, metabolomics, and microbiomics, which are collectively referred to as omics platforms, have led to the collection of substantial amounts of data. Integration of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important traits. Several attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of chrysanthemum. However, applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge, primarily due to the high heterozygosity and polyploidy of the species. This review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum.

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Characterization of TEMINAL FLOWER1 homologs CmTFL1c gene from Chrysanthemum morifolium

Cited by 21 publications

References 42 publications

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Characterization of the BnA10.tfl1 Gene Controls Determinate Inflorescence Trait in Brassica napus L.

Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

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