Characterization of supercritical fluid extracts of St. John’s Wort (Hypericum perforatum L.) by HPLC–MS and GC–MS

Quality control of Hypericum perforatum L. analytical challenges and recent progress

“…A method was developed for the LC‐MS determination of apolar compounds in supercritical extracts (and also suitable for the DAD quantification of hyperforin and adhyperforin); 16 hyperforin derivatives were identified by LC‐MS in ESI‐negative and ESI‐positive ionization modes, and DAD determination of hyperforin and adhyperforin was also carried out with LOD and LOQ values of 4.1 and 2.3 μg/ml, 13.4 and 7.8 μg/ml, respectively …”

“…[91] A method was developed for the LC-MS determination of apolar compounds in supercritical extracts (and also suitable for the DAD quantification of hyperforin and adhyperforin); 16 hyperforin derivatives were identified by LC-MS in ESI-negative and ESI-positive ionization modes, and DAD determination of hyperforin and adhyperforin was also carried out with LOD and LOQ values of 4.1 and 2.3 lg/ml, 13.4 and 7.8 lg/ml, respectively. [92] Some methods are dedicated to the analysis of biological samples from human trials. A sensitive LC-MS-MS method for the simultaneous determination of hypericin and hyperforin was validated with human plasma samples.…”

Analytical approaches for the detection of epoxides and hydroperoxides in active pharmaceutical ingredients, drug products and herbals

“…An Austrian research group [18] used supercritical fluid extraction techniques to concentrate lipophilic plant constituents from St. John's wort (Hypericium perforatum L) that were subsequently identified by HPLC-MS and GC-MS. In addition to the principal phloroglucinols, hyperforin (36.5%) and adhyperforin (4.6%), there was a proliferation of oxidation products.…”

Liquid chromatography–mass spectrometry studies of St. John's wort methanol extraction: active constituents and their transformation

“…The use of LC-MS for identification of hyperforin transformation products provides a fast and direct analysis, which is the major benefit of LC-MS compared to NMR. Although a number of papers were published on the application of LC-MS to the St. John's wort extraction [9][10][11][12][13][14], most of the studies focused on the quantitative applications. [5].…”

“…St. John's wort contains groups of naphthodianthrones and phloroglucinols, which contribute to its antidepressant activity. Numerous papers have been published in the areas of isolation and purification of hyperforin [1], supercritical carbon dioxide extraction [2], HPLC analytical assay [3][4][5], capillary electrophoresis [6], optimization of extraction conditions [7], NIR rapid quantification [8], characterization of hyperforin transformation products using mass spectrometry [9][10][11][12][13][14] and nuclear magnetic resonance (NMR) spectroscopy [15][16][17][18][19]. 1 elucidation of transformation products due to their detailed structural information.…”