Characterization of subunits encoded by SnRK1 and dissection of combinations among these subunits in sorghum (Sorghum bicolor L.)

Xiao, Qianlin; Huang, Tianhui; ZHOU, Chang; CHEN, Wei-xi; CHA, Jian-kui; WEI, Xi-mei; Xing, Fangyu; QIAN, Meng-ya; Ma, Qiannan; Duan, Hong; LIU, Zhi-zhai

doi:10.1016/j.jia.2022.08.068

Cited by 3 publications

(2 citation statements)

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“…In contrast, overexpression of AtSnRK1α1 resulted in late flowering and enhanced plant tolerance to nitrogen or carbon starvation, implying their important role in plant growth and stress response [12]. SnRK1α1 is mainly responsible for the activity of SnRK1 [13]; SnRK1α2 is rarely reported, while SnRK1α3 is only expressed at low levels in pollen and seeds [10]. The catalytic α-subunit (SnRK1α1/KIN10 and SnRK1α2/KIN11 in Arabidopsis) consists of an N-terminal Ser/Thr kinase structural domain linked to the C-terminal regulatory structural domain, which interacts with the regulatory subunit [14].…”

Section: Introductionmentioning

confidence: 99%

“…Eukaryotic AMPK/SNF1/SnRK1 protein kinase is a heterotrimeric complex with catalytic α subunit and regulation of β and γ subunits [7][8][9]. There are three genes encoding α subunits, AtSnRK1α1, AtSnRK1α2, and AtSnRK1α3, in Arabidopsis [10]. The stomatal index is reduced in the snrk1α1 mutant of Arabidopsis, and its overexpression leads to an elevated stomatal index.…”

Section: Introductionmentioning

confidence: 99%

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A Soybean Sucrose Non-Fermenting Protein Kinase 1 Gene, GmSNF1, Positively Regulates Plant Response to Salt and Salt–Alkali Stress in Transgenic Plants

Lu,

Dai,

Yong

et al. 2023

IJMS

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Soybean is one of the most widely grown oilseed crops worldwide. Several unfavorable factors, including salt and salt–alkali stress caused by soil salinization, affect soybean yield and quality. Therefore, exploring the molecular basis of salt tolerance in plants and developing genetic resources for genetic breeding is important. Sucrose non-fermentable protein kinase 1 (SnRK1) belongs to a class of Ser/Thr protein kinases that are evolutionarily highly conserved direct homologs of yeast SNF1 and animal AMPKs and are involved in various abiotic stresses in plants. The GmPKS4 gene was experimentally shown to be involved with salinity tolerance. First, using the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique, the GmSNF1 protein was shown to interact with the GmPKS4 protein. Second, the GmSNF1 gene responded positively to salt and salt–alkali stress according to qRT-PCR analysis, and the GmSNF1 protein was localized in the nucleus and cytoplasm using subcellular localization assay. The GmSNF1 gene was then heterologously expressed in yeast, and the GmSNF1 gene was tentatively identified as having salt and salt–alkali tolerance function. Finally, the salt–alkali tolerance function of the GmSNF1 gene was demonstrated by transgenic Arabidopsis thaliana, soybean hairy root complex plants overexpressing GmSNF1 and GmSNF1 gene-silenced soybean using VIGS. These results indicated that GmSNF1 might be useful in genetic engineering to improve plant salt and salt–alkali tolerance.

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