Characterization of stiffness of micro-particle trapped in dielectrophoretic-microfluidic device

Novel Approaches Concerning the Numerical Modeling of Particle and Cell Separation in Microchannels: A Review

“…This is a specific frequency at which the intrinsic properties of particles and cells can be defined [15]. For the separation or isolation of cells and biological material, it is desirable to employ nDEP so that the material is directed towards the low electric field region to minimize unwanted transfection and disruption of cell structure/membrane [11]. It should also be noted that while the electric field induces DEP forces on the particles, it also induces an electro-osmotic and electrothermal flow that might affect the separation [45].…”

“…Recent research has been devoted to the manipulation, concentration, focusing, separation, isolation, and fractionation of particulate material such as micro-and nanoparticles, cells, liposomes, microvesicles, viruses, etc., using microfluidic devices, i.e., the so-called lab on chip devices [5][6][7]. Microfluidics-based cell/particle manipulation technologies have shown great potential due to the low sample and reagent volume consumption, low waste generation, high product purity, high sensitivity and selectivity, ease of use, and short isolation time, in addition to being able to perform particle/cell analysis at the single-cell level [8][9][10][11][12][13]. Moreover, downscaling of sorting systems enables less laboratory space, and the integration of multiple tasks and operations within the same device is possible, which not only increases the precision of the analysis but also improves the accuracy, reliability, and reproducibility of sample preparation procedures [14,15].…”

“…As depicted in Figure 1, more than 600 papers have been published since 2003, most of them reported in the last 5 years. To provide a global overview of the state of the art, nearly one hundred scientific publications published in the last 5 years (from 2017 to 2021) and employing novel models for the description of acoustophoresis, magnetophoresis, and dielectrophoresis (the most common active techniques for the separation of cells and particles) have been reviewed [1][2][3]5,8,9,11,[14][15][16]19,20,24,26,27,. ) related to the us of numerical modeling for the microfluidic separation of particles and cells, and found in the S pus database using the keywords ''cell separation", "particle separation", "simulation", and "m crofluidics".…”