Characterization of Sperm Immobilization Factor from Escherichia coli and Its Receptor to Study the Underlying Mechanism of Sperm Immobilization

Vander, Harpreet; Gupta, Sonia; Kaur, Sukhdeep; Kaur, Kiranjeet; Prabha, Vijay

doi:10.5099/aj130100025

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…In recent studies, researchers have reported that E. coli contains a large SIF, a heat-labile, 56 kDa protein that can recognize a specific 113 KDa receptor presented on the membrane of spermatozoa ( Prabha et al., 2010 ; Kumar et al., 2011 ). This SIF has a significant inhibitory effect on Mg 2+ -dependent ATPase activity and acrosome reaction induced by calcium ionophore ( Vander et al., 2013 ).…”

Section: Types and Pathogenic Properties Of The Semen Microbiomementioning

confidence: 99%

Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Zhang

Dai

Chen

2021

Front. Cell. Infect. Microbiol.

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Infertility has become a common problem in recent decades. The pathogenesis of infertility is variable, but microbiological factors account for a large proportion of it. Dysbiosis of vaginal microbiota is reportedly associated with female infertility, but the influence of normal vaginal microbiota on infertility is unclear. In this review, we summarize the physiological characteristics of the vaginal tract and vaginal microbiota communities. We mainly focus on the bacterial adherence of vaginal Lactobacillus species. Given that the adherent effect plays a crucial role in the colonization of bacteria, we hypothesize that the adherent effect of vaginal Lactobacillus may also influence the fertility of the host. We also analyze the agglutination and immobilization effects of other bacteria, especially Escherichia coli, on ejaculated spermatozoa, and speculate on the possible effects of normal vaginal microbiota on female fertility.

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Section: Types and Pathogenic Properties Of The Semen Microbiomementioning

confidence: 99%

Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Zhang

Dai

Chen

2021

Front. Cell. Infect. Microbiol.

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“…Finally, an almost non-existent sperm motility and mitochondrial activity accompanied by the highest degree of disruption to the membranous structures of rooster spermatozoa early on in the sperm–bacteria co-culture may have been caused by the sperm agglutinating factor and the sperm immobilizing factor secreted by E. coli . Both molecules have been reported to inhibit Mg 2+ -dependent ATPase [ 53 ] and to promote premature acrosome reactions by causing ionic imbalances in sperm cells [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Attenuates Damage to Rooster Spermatozoa Exposed to Selected Uropathogens

Tvrdá

Petrovičová²,

Benko³

et al. 2022

Pharmaceutics

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Artificial insemination, as an essential pillar of the modern poultry industry, primarily depends on the quality of semen collected from stud roosters. Since the collection and storage of ejaculates is not a sterile process, antimicrobial agents have become essential supplements to semen extenders. While the use of traditional antibiotics has been challenged because of rising bacterial resistance, natural biomolecules represent an appealing alternative because of their antibacterial and antioxidant properties. As such, this study strived to compare the effects of 50 μmol/L curcumin (CUR) with 31.2 µg/mL kanamycin (KAN) as a conventional antibiotic on rooster sperm quality in the presence of Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa. Changes in sperm structural integrity and functional activity were monitored at 2 and 24 h of culture. Computer-assisted semen analysis revealed significant sperm motility preservation following treatment with KAN, particularly in the case of Salmonella enterica and Pseudomonas aeruginosa (p < 0.001) after 24 h. On the other hand, CUR was more effective in opposing ROS overproduction by all bacteria (p < 0.05), as determined by luminol-based luminometry, and maintained sperm mitochondrial activity (p < 0.001 in the case of Salmonella enterica; p < 0.05 with respect to Escherichia coli and Pseudomonas aeruginosa), as assessed by the fluorometric JC-1 assay. The TUNEL assay revealed that CUR readily preserved the DNA integrity of rooster sperm exposed to Salmonella enterica (p < 0.01) and Escherichia coli (p < 0.001). The bacteriological analysis showed higher efficiency of KAN in preventing the growth of all selected bacterial species (p < 0.0001) as opposed to CUR. In conclusion, CUR provided protection to rooster spermatozoa against alterations caused by uropathogens, most likely through its antioxidant activity. Hence, CUR supplementation to poultry semen extenders in combination with properly selected antibacterial substances may become an interesting strategy in the management of bacterial contamination during semen storage.

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“…As matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and 16S ribosomal RNA sequencing have been applied to study the human microbiome, associations between the bacterial profiles of semen and sperm quality have been gradually revealed [ 21 , 22 , 30 , 31 ]. Bacteria may damage male reproductive cells either by direct cell-to-cell interactions through adhesion [ 32 ] leading to sperm immobilization and agglutination [ 33 , 34 , 35 ] or by the release of cytotoxic endotoxins [ 36 ]. Oxidative stress and leukocytospermia as side-effects of bacteriospermia may equally contribute to a lower semen quality [ 22 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men—How Does the Seminal Bacteriome Respond?

Tvrdá

Ďuračka

Benko

et al. 2023

IJMS

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This study was designed to describe bacterial profiles of ejaculates collected following a long and short ejaculatory abstinence set in the context of changes in the conventional, oxidative, and immunological characteristics of semen. Two specimens were collected in succession from normozoospermic men (n = 51) following 2 days and 2 h, respectively. Semen samples were processed and analyzed according to the World Health Organization (WHO) 2021 guidelines. Afterwards, sperm DNA fragmentation, mitochondrial function, levels of reactive oxygen species (ROS), total antioxidant capacity, and oxidative damage to sperm lipids and proteins were evaluated in each specimen. Selected cytokine levels were quantified using the ELISA method. Bacterial identification by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry revealed that samples collected following two days of abstinence presented with a higher bacterial load and diversity, and a greater prevalence of potentially uropathogenic bacteria including Escherichia coli, Staphylococcus aureus and Enterococcus faecalis. Only staphylococci and Escherichia coli remained present in specimens obtained after 2 h of abstinence. Whilst all samples accomplished the criteria set by WHO, a significantly higher motility (p < 0.05), membrane integrity (p < 0.05), mitochondrial membrane potential (p < 0.05), and DNA integrity (p < 0.0001) were detected following 2 h of ejaculatory abstinence. On the other hand, significantly higher ROS levels (p < 0.001), protein oxidation (p < 0.001), and lipid peroxidation (p < 0.01) accompanied by significantly higher concentrations of tumor necrosis factor alpha (p < 0.05), interleukin-6 (p < 0.01), and interferon gamma (p < 0.05) were observed in specimens collected after two days of abstinence. It may be summarized that shorter ejaculatory abstinence does not compromise sperm quality in normozoospermic men, while it contributes to a decreased occurrence of bacteria in semen which is accompanied by a lower probability of damage to spermatozoa by ROS or pro-inflammatory cytokines.

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Characterization of Sperm Immobilization Factor from Escherichia coli and Its Receptor to Study the Underlying Mechanism of Sperm Immobilization

Cited by 5 publications

References 17 publications

Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Curcumin Attenuates Damage to Rooster Spermatozoa Exposed to Selected Uropathogens

Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men—How Does the Seminal Bacteriome Respond?

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