Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Zhang, Fenghao; Dai, Jie; Chen, Tingtao

doi:10.3389/fcimb.2020.620529

Cited by 28 publications

(21 citation statements)

References 112 publications

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“…The vaginal microbiome has been studied in pregnancy ( Romero et al., 2014 : Mehta et al., 2020 ), cervical cancer ( Champer et al., 2018 ; Chambers et al., 2021 ; and infertility ( Zhang et al., 2021 ). Alteration in the vaginal ecology during which the commensal lactobacilli are reduced and displaced by the anaerobic species of the genera Gardnerella, Sneathia, Prevotella, Atopobium.…”

Section: Introductionmentioning

confidence: 99%

Vaginal Dysbiotic Microbiome in Women With No Symptoms of Genital Infections

Pramanick

Nathani

Warke

et al. 2022

Front. Cell. Infect. Microbiol.

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The vaginal microbiome plays a critical role in determining the progression of female genital tract infections; however, little is known about the vaginal microbiota of Indian women. We aimed to investigate the vaginal microbial architecture of women with asymptomatic bacterial vaginosis (BV) (n=20) and normal microbiota (n=19). Microbial diversity was analyzed in vaginal swabs from regularly menstruating women (18-45yrs) by 16S rRNA V3-V4 amplicon (MiSeq Illumina) sequencing. Rarefaction analysis showed a higher number of species in normal flora compared to BV. Alpha diversity as measured by Pielou’s evenness revealed microbial diversity was significantly greater in BV samples than normal microbiota (p= 0.0165). Beta diversity comparison using UniFrac metrics indicated distinct microbial communities clustering between normal and BV flora. Firmicutes were the major phyla observed in vaginal specimens of normal microbiota whereas Actinobacteria, Fusobacteria, Bacteroidetes were significantly abundant in BV samples. Notably, the relative abundance of Lactobacillus was significantly high in normal microbiota. Conversely Gardnerella, Sneathia, Prevotella, Atopobium, Ureaplasma, Dialister significantly dominated dysbiotic microbiota. Relative frequency of Lactobacillus decreased significantly in BV (6%) as compared to normal microbiota (35.2%). L. fermentum, L. gasseri, L. iners, L. jensenii, L. mucosae, L. ruminis, L. salivarius, L. coleohominis was more exclusively present in normal microbiota. L. iners was detected from both the groups with a relative frequency of 50.4% and 17.2% in normal and BV microbiota respectively. Lefse analysis indicated Atopobium vaginae, Sneathia amnii, Mycoplasma hominis Prevotella disiens in the vaginal microbiota as a biomarker for dysbiosis and L. jensenii as a biomarker of a healthy microbiota. Firmicutes were negatively correlated to Tenericutes, Actinobacteria, Bacteroidetes, and Fusobacteria. Proteobacteria positively correlated to Tenericutes, and Bacteroidetes were shown to be positively correlated to Fusobacteria. Predicted functional analysis indicated differences in the functional profiles between BV and normal microbiota. Normal microbiota utilized pathways essential for phosphatidylglycerol biosynthesis I & II, peptidoglycan biosynthesis, geranylgeranyl diphosphate biosynthesis I, mevalonate pathway, CoA biosynthesis pathway I and pyrimidine nucleotide salvage; whereas BV bacteria had characteristic aromatic amino acid biosynthesis, pentose phosphate pathway, carbohydrate degradation. In conclusion, women with asymptomatic BV have vaginal microbiota significantly different than women with normal microbiota. Furthermore, the study provides insights into the vaginal microbial structure of Indian women that will enable us to explore the prospective candidates for restoring the vaginal microbiota.

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Section: Introductionmentioning

confidence: 99%

Vaginal Dysbiotic Microbiome in Women With No Symptoms of Genital Infections

Pramanick

Nathani

Warke

et al. 2022

Front. Cell. Infect. Microbiol.

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“…A comparative genomic analysis of E. coli strains used in our study revealed the unique presence in E. coli ATCC 35218 of the chromosomal HlyE gene coding for hemolysinE (data not shown), a toxin with a short half-life that is known to impair membrane integrity in other cell types 40 . Therefore, it is possible that the detrimental effects of E. coli strain ATCC 35218 are mediated by more than one spermotoxic factor 41 .…”

Section: Discussionmentioning

confidence: 99%

Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions

Marchiani

Baccani

Mattiuz

et al. 2021

Sci Rep

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Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic species (two E.coli strains, three K. pneumoniae strains, P. aeruginosa and E. cloacae) on motility, viability, mitochondrial oxidative status, DNA fragmentation and caspase activity of human spermatozoa. All strains, except P. aeruginosa, reduced significantly sperm motility, with variable effects. Sperm Immobilizing Factor (SIF) was largely responsible for deteriorating effects on sperm motility of E. coli strains since they were completely reverted by knockout of SIF coding recX gene. Sequence alignment for RecX showed the presence of high homologous sequences in K. pneumoniae and E. cloacae but not in P. aeruginosa. These results suggest that, in addition to E.coli, other common uropathogenic Gram-negative bacteria affect sperm motility through RecX products. In addition to sperm motility, the E. coli strain ATCC 35218 also affected sperm viability, and induced caspase activity, oxidative stress and DNA fragmentation suggesting an interspecies variability in the amount and/or type of the produced spermatotoxic factors. In general, our results highlight the need for a careful evaluation of semen infections in the diagnostic process of the infertile man.

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“…However, many unexplained cases of infertility could also be attributed to the vaginal microbiome. Researchers have put forward many hypothesis to explain it, including the impact of vaginal bacteria on sperm motility, [ 25 ] bioactive metabolites of the vaginal microbiome entering the blood to cause fecundability reduction, [ 26 ] and the potential effect of the vaginal microbiome on the hypothalamic pituitary ovarian axis [ 27 ]. Although there is no direct evidence that M.hominis would impact female fertility through above three pathways, these hypotheses are also reasonable for M.hominis .…”

Section: Discussionmentioning

confidence: 99%

The associations between low abundance of Mycoplasma hominis and female fecundability: a pregnancy-planning cohort study

et al. 2022

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Objective To explore the impact of pre-pregnancy vaginal Mycoplasma hominis (M. hominis) colonization of low abundance on female fecundability. Methods In total, 89 females participating in a pre-pregnancy health examination program were included, and their pregnancy outcomes were followed up for 1 year. Vaginal swabs were collected, 16S rRNA genes were sequenced, and M. hominis colonization was confirmed by qPCR. Cox models were used to estimate the fecundability odds ratio (FOR) for women with M. hominis. Results The prevalence of M. hominis was 22.47% (20/89), and the abundance was relatively low (the cycle thresholds of the qPCR were all more than 25). In terms of the vaginal microbiome, the Simpson index of the positive group was significantly lower than that of the negative group (P = 0.003), which means that the microbiome diversity appeared to increase with M. hominis positivity. The relative abundance of M. hominis was negatively correlated with Lactobacillus crispatus (rho = − 0.24, P = 0.024), but positively correlated with Gardnerella vaginalis, Atopobium vaginae and Prevotella bivia (P all < 0.05). The cumulative one-year pregnancy rate for the M. hominis positive group was lower than that in the negative group (58.96% vs 66.76%, log-rank test: P = 0.029). After controlling for potential confounders, the risk of pregnancy in the M. hominis positive group was reduced by 38% when compared with the positive group (FOR = 0.62, 95% CI: 0.42–0.93). Conclusion The vaginal colonization of M. hominis at a low level in pre-pregnant women is negatively correlated with female fecundability.

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Role of Lactobacillus in Female Infertility Via Modulating Sperm Agglutination and Immobilization

Cited by 28 publications

References 112 publications

Vaginal Dysbiotic Microbiome in Women With No Symptoms of Genital Infections

Vaginal Dysbiotic Microbiome in Women With No Symptoms of Genital Infections

Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions

The associations between low abundance of Mycoplasma hominis and female fecundability: a pregnancy-planning cohort study

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