Characterization of Some Italian Common Bean (Phaseolus Vulgaris L.) Landraces by RAPD, Semi-random and ISSR Molecular Markers

Abstract

Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and a semi-random PCR system were used to analyze the genetic diversity of 16 Italian common bean landraces and their relationship to four commercial cultivars. Of the primers tested, 8 ISSR, 6 RAPD and 7 semi-random primers produced polymorphic and reproducible DNA fragments. A higher proportion of polymorphic bands were observed using ISSR (85%) and semi-random (90%) primers than RAPD (69%) method. The combination of any two semi-random markers allowed the identification of all 20 bean genotypes. In contrast ISSR (except for primer (CAC) 3 GC) and RAPD markers appeared to be less informative as more than two markers were necessary to achieve the same diagnostic level. Moreover, 7 ISSR, 2 RAPD and 8 semi-random exclusive bands were identified as putative population-specific markers. Semi-random and ISSR derived dendrograms showed similar tendencies in terms of genetic relatedness, whereas clustering of genotypes within groups was not similar when compared with the RAPD technique. Despite the different ability to resolve genetic variation among the investigated landraces, two major clusters with less than 60% (ISSR) and 40% (RAPD and semi-random) genetic similarity were formed with all three marker systems. The two groups were correlated with the phaseolin patterns and seed size of the landraces. The analysis showed that the cultivar 'Lingua di Fuoco' and most of the landraces (13 out of 16) collected in Italy belong to the Andean gene pool, whereas only the three populations from Pratomagno belong to the Middle American gene pool.

Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces

“…Comparative studies in Vigna species involving RAPD, AFLP, ISSR and SSR marker systems were successfully used by very limited researchers (Souframanien and Gopalakrisna, 2004;Gillaspie et al 2005;Dikshit et al 2007). But, it has been carried out in many crops like rice (Ravi et al 2003), capsicum (Ruanet et al 2005) and common bean (Marotti et al 2006). The discriminative power of DNA markers used as tool to characterize the rice bean landraces is very important because they can be used to assess the genetic diversity among the landraces of rice bean.…”

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

“…These are randomly selected with the advantage of analyzing multiple loci in a single PCR and not requiring any prior information on the target sequence in the genome. They have been widely used in recent years in wheat, bean, firs, and tea (Hailu et al, 2005;Marotti et al, 2007;Zhang et al, 2007;Liu et al, 2010).…”

Distinctiveness of Bean Landraces in Italy: the Case Study of the ‘Badda’ Bean

“…Several classes of genetic markers have been used in order to infer genetic variability, phylogeny and distinctiveness in common bean, including biochemical polymorphisms [2,6,8,14] and molecular markers such as random amplified polymorphic DNAs (RAPDs) [15,16], amplified fragment length polymorphisms (AFLPs) [5,9,15,17,18], simple sequence repeats (SSRs) [9,10,12,18] and inter simple sequence repeats (ISSRs) [10,16,19,20]. Here, we adopt ISSR marker analysis on DNA bulks from different plants, in order to maximize cost-effectiveness and information for landrace distinction.…”