Characterization of some Egyptian Sheep Populations Using Microsatellite and Protein Markers

Abstract

Three Egyptian sheep populations, collected from three geographically isolated regions namely Siwa, El-Dakhla and El-Farafra oases of the Egyptian western desert, were investigated using six polymorphic microsatellite markers and electrophoretic protein by SDS-PAGE. Six polymorphic microsatellite markers and electrophoretic protein were used to reveal that the genetic diversity, conduct genetic structure and assignment of microsatellite. The results indicated that; one hundred and five alleles were detected; 34 are common alleles and 71 specific alleles across six loci (67.62%). Thirteen specific alleles for Siwa sheep population ranged from 1 to 5. Also, for Dakhla sheep population 21 alleles ranged from 1 to 8. While 37 ones were obtained in the case of Farafra sheep population ranging from 1 to 14. When the heterozygotsity is high we will have the highest effective number of alleles (ENA) and the expected heterozygosity (gene diversity). The highest ENA was 10.29 for BM1314 when HE was 0.94, while the lowest ENA was 2.22 for BM8125 when HE was 0.58 with El-Dakhla sheep population. The protein profile for 33 sheep population samples collected from three different regions, Siwa had 20 protein bands that MW ranged from 17 to 269KDa. At the same concentration of protein and molecular weight, the band's volume or intensity values changed from sample to other. The homogeneity of individual Siwa samples was 30%. El-Dakhla population had 23 protein bands that ranged from17 to 283KDa. The heterogeneity's El -Dakhla population was 74%. While, Elfarfra populations varied in electrophoretic protein pattern, it had 21 bands that ranged from 17 to 252 KDa. The homogeneity percentage was 24%. Finally, protein profile can be used as a marker depending on the presence or absence bands, band intensity, molecular weight and relative front values.

Genetic variation of myostatin MSTN gene and protein polymorphism in three goat populations

“…and Beetal breed has band of 36.5 kDa). El-Hamamsy et al (2018) reported that the Siwa had 20 protein bands that MW ranged from 17 to 269 KDa, the homogeneity of individual Siwa samples was 30%. El-Dakhla population had 23 protein bands that ranged from 17 to 283KDa.…”

“…A polyacrylamide gel electrophoresis is a valuable tool for establishing the quantitative distribution of proteins in goats (Shauket et al, 1998;Simplício et al, 2017) illustrated that the detection and quantification of acute-phase proteins by SDSP-AGE was useful and reliable as a complementary test in goats. The protein profile can be used as a marker depending on the presence or absence bands, band intensity, molecular weight and relative front values (El-Hamamsy et al, 2018). In addition, the coding sequences of bovine myostatin in two double-muscled cattle's have been identified, as well as nine other vertebrate species' myostatin sequences (Belgian Blue and Piedmontese) (Mcpherron et al 1997).…”

Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds

“…According to a literature survey, several papers concerning its morphological characterization (Mousa et al, 2006;Galal, 2007;Elbeltagy, 2012;Elshazly and Youngs, 2019). Moreover, only one available molecular study was performed by El-Hamamsy et al (2018). They characterized three Egyptian sheep populations, collected from Siwa, El-Dakhla and El-Farafra Oases of the Egyptian Western Desert, using microsatellite marker and electrophoretic protein.…”