Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalents

Hu, Shijia; Muniraj, Giridharan; Mishra, Apurva; Hong, Kanglun; Lum, Jing Li; Hong, Cassandra; Rosa, Vinícius; Sriram, Gopu

doi:10.1016/j.dental.2022.06.025

Cited by 28 publications

(29 citation statements)

References 40 publications

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“…This critical step may be the reason why static culture conditions were chosen, instead of including saliva flow and/or blood flow in dental pulp. Recently, the physiological blood flow was simulated in a vertical bilayer chip, where a dentin disc was clamped above a rhomboid-shaped culturing chamber for DPSCs, with a flow channel in between ( Hu et al 2022 ). In this way, the part of the flow from the inlet toward the disc/cells could be analyzed and serve as the internal control and the section of the flow thereafter as the experimental situation.…”

Section: Oocs In Doc Researchmentioning

confidence: 99%

“…Thermoplastic chips are alternatives for quantitative experiments. Poly-methylmethacrylate chips fabricated by micromilling have been used as tooth-on-a-chip or skin-on-a-chip for toxicological applications ( Sriram et al 2018 ; Hu et al 2022 ). In industrial settings, injection molding and embossing are popular to decrease fabrication cost and achieve upscalability ( Low et al 2021 ).…”

Section: Introduction Of Oocsmentioning

confidence: 99%

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The Application of Organs-on-a-Chip in Dental, Oral, and Craniofacial Research

et al. 2023

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The current development of microfluidics-based microphysiological systems (MPSs) will rapidly lead to a paradigm shift from traditional static 2-dimensional cell cultivation towards organized tissue culture within a dynamic cellular milieu. Especially organs-on-a-chip (OoCs) can very precisely re-create the mechanical and unique anatomical structures of the oral environment. This review provides an introduction to such technology, from commonly used chip materials and fabrication methods to the application of OoC in in vitro culture. OoCs are advantageous because of their small-scaled culture environment, the highly controlled dynamic experimental conditions, and the likeness to the in vivo structure. We specifically focus on current chip designs in dental, oral, and craniofacial (DOC) research. Also, future perspectives are discussed, like model standardization and the development of integrated platforms with advanced read-out functionality. By doing so, it will be possible for OoCs to serve as an alternative for animal testing and to develop highly predictive human models for clinical experiments and even personalized medicine.

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Section: Oocs In Doc Researchmentioning

confidence: 99%

Section: Introduction Of Oocsmentioning

confidence: 99%

The Application of Organs-on-a-Chip in Dental, Oral, and Craniofacial Research

et al. 2023

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“…[ 1,5 ] Existing standards are commonly based on assays using monolayer cultures of a single cell type. In recent years, there has been a significant impetus toward the development and application of in vitro three‐dimensional (3D) organotypic models that emulate the complex microenvironment of the gingiva and oral mucosa such as reconstructed epithelium, [ 6–8 ] connective tissue [ 9 ] and full‐thickness gingival [ 10–15 ] equivalents. These 3D models offer a physiological alternative to understanding the host‐material and host‐microbial interactions.…”

Section: Introductionmentioning

confidence: 99%

Microphysiological Modeling of Gingival Tissues and Host‐Material Interactions Using Gingiva‐on‐Chip

Muniraj,

Tan,

Dai

et al. 2023

Adv Healthcare Materials

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Gingiva plays a crucial barrier role at the interface of teeth, tooth‐supporting structures, microbiome, and external agents. To mimic this complex microenvironment, we developed an in vitro microphysiological platform and biofabricated full‐thickness gingival equivalents (gingiva‐on‐chip) within a vertically‐stacked microfluidic device. This design allowed long‐term and air‐liquid interface culture, and host‐material interactions under flow conditions. Compared to static cultures, dynamic cultures on‐chip enabled the biofabrication of gingival equivalents with stable mucosal matrix, improved epithelial morphogenesis, and barrier features. Additionally, a diseased state with disrupted barrier function representative of gingival/oral mucosal ulcers was modeled. The apical flow feature was utilized to emulate the mechanical action of mouth rinse and integrate the assessment of host‐material interactions and transmucosal permeation of oral‐care formulations in both health and diseased states. Although the gingiva‐on‐chip cultures had thicker and more mature epithelium, the flow of oral‐care formulations induced increased tissue disruption and cytotoxic features compared to static conditions. The realistic emulation of mouth rinsing action facilitated a more physiological assessment of mucosal irritation potential. Overall, this microphysiological system enables biofabrication of human gingiva equivalents in intact and ulcerated states, providing a miniaturized and integrated platform for downstream host‐material and host‐microbiome applications in gingival and oral mucosa research.This article is protected by copyright. All rights reserved

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“…45,49 Similarly, it has been utilized to mimic the nutrient provision and metabolic waste or toxic product removal role of the blood vessels. 50,51 , 76…”

Section: Introductionmentioning

confidence: 99%