Characterization of sickness behavior in zebrafish

Kirsten, Karina; Soares, Suelen Mendonça; Koakoski, Gessi; Kreutz, Luiz Carlos; Barcellos, Leonardo José Gil

doi:10.1016/j.bbi.2018.07.004

Cited by 51 publications

(67 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An induced inflammatory response by inoculating fish with formalin-inactivated Aeromo nas hydrophila reduced social preference behavior (Kirsten et al, 2018), consistent with a previous report linking the immune system with social behavior in mice (Filiano et al, 2016). Traumatic brain injury (TBI) by pulsed, high-intensity focused ultrasound to the adult zebrafish brain increased shoaling cohesion (McCutcheon et al, 2017), although it may be difficult to determine the exact location and degree of brain damage caused by such a diffusive injury method.…”

Section: Disease-relevant Social-deficit Models In Zebrafishsupporting

confidence: 90%

The zebrafish subcortical social brain as a model for studying social behavior disorders

Geng

Peterson

2019

Disease Models &Amp; Mechanisms

View full text Add to dashboard Cite

Social behaviors are essential for the survival and reproduction of social species. Many, if not most, neuropsychiatric disorders in humans are either associated with underlying social deficits or are accompanied by social dysfunctions. Traditionally, rodent models have been used to model these behavioral impairments. However, rodent assays are often difficult to scale up and adapt to high-throughput formats, which severely limits their use for systems-level science. In recent years, an increasing number of studies have used zebrafish (Danio rerio) as a model system to study social behavior. These studies have demonstrated clear potential in overcoming some of the limitations of rodent models. In this Review, we explore the evolutionary conservation of a subcortical social brain between teleosts and mammals as the biological basis for using zebrafish to model human social behavior disorders, while summarizing relevant experimental tools and assays. We then discuss the recent advances gleaned from zebrafish social behavior assays, the applications of these assays to studying related disorders, and the opportunities and challenges that lie ahead.

show abstract

Section: Disease-relevant Social-deficit Models In Zebrafishsupporting

confidence: 90%

The zebrafish subcortical social brain as a model for studying social behavior disorders

Geng

Peterson

2019

Disease Models &Amp; Mechanisms

View full text Add to dashboard Cite

show abstract

“…Fish injected with the medium and high antigen doses decreased their swimming activity after 4 days. This is consistent with the expected increase in energetic demand for the immune response (Kirsten et al, 2018). However, at the lowest antigen concentration, fish swimming activity was not affected by the immune challenge, which suggests a dose‐dependent effect of antigens on fish behavior.…”

Section: Discussionsupporting

confidence: 89%

“…However, at the lowest antigen concentration, fish swimming activity was not affected by the immune challenge, which suggests a dose‐dependent effect of antigens on fish behavior. This supports that immune challenge with medium and high doses of antigens increases immunity at the expense of swimming activity, which is a classical sickness behavior allowing fish to save energy for immunity (Dantzer, 2004; Johnson, 2002; Kirsten et al, 2018). However, a potential side effect is that less active fish could be less able to find food (Dantzer, 2004; Johnson, 2002; Volkoff & Peter, 2004), which could explain the transient reduction of available energy stored (i.e., at 4 days) in the muscle of antigen‐injected fish (Volkoff & Peter, 2004).…”

Section: Discussionmentioning

confidence: 53%

“…The so‐called “sickness behavior” is a suite of behavioral changes, including a decrease of swimming activity, exploration, and social behavior that can enable hosts to save energy for immunity and/or to limit the exposure to pathogens (Adelman & Martin, 2009; Combes, 2001). For instance, Kirsten et al (2018) recently showed that immune‐challenged zebrafish ( Danio rerio ) displayed reduced swimming activity, sociability, and exploratory behavior, which could benefit individuals by saving energy for immunity, but could also have severe negative consequences for fish fitness by reducing fish ability to forage, find mates, or escape predators (Wong & Candolin, 2015). However, the threshold (e.g., the amount of antigens required) and the timing (i.e., duration) at which immune challenges trigger such behavioral changes are still unclear.…”

Section: Introductionmentioning

confidence: 99%

“…More generally, few studies investigated the cascading effects of immune challenges across levels of biological organization (i.e., from molecules to the whole individual), especially in fish, which limits our ability to predict the impacts of pathogens on fish fitness (Kirsten et al, 2018). Empirical studies are thus needed to test the potential physiological and behavioral changes in immune‐challenged fish across time and biological levels.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dose‐ and time‐dependent effects of an immune challenge on fish across biological levels

et al. 2020

View full text Add to dashboard Cite

Due to global changes, fish are increasingly exposed to immune challenges associated with disease outbreaks in aquatic ecosystems. Adjustments in physiology and behavior are generally critical to maintaining homeostasis after an immune challenge, but there is limited knowledge on the specific thresholds and dynamics of responses across levels of biological organization in fish. In this study, we tested how different concentrations of an antigens mixture (phytohemagglutinin and lipopolysaccharide) affected innate immunity with potential consequences on oxidative stress, energy reserves, body condition, and behavior across time, using the common gudgeon (Gobio sp.) as model species. The immune challenge induced a transitory increase in lytic enzyme activity (i.e., lysozyme) and local immune response (i.e., skin swelling) 2 days after the antigen injection. The available energy stored in muscle was also reduced 4 days after injection, without inducing oxidative stress at the cellular level. Overall, the immune challenge induced limited costs at the molecular and cellular levels but had strong effects at the whole organism level, especially on behavior. Indeed, fish swimming activity and sociability were affected in a dose‐ and time‐dependent manner. These results suggest that immune challenges have dose‐dependent effects across levels of biological organization and that behavior is a key response trait to cope with pathogen‐induced immune costs in the wild, although fitness consequences remain to be tested.

show abstract

Pain and Sickness Behaviors

Stella¹,

Buffington²

2022

Clinical Handbook of Feline Behavior Medicine

View full text Add to dashboard Cite

Characterization of sickness behavior in zebrafish

Cited by 51 publications

References 45 publications

The zebrafish subcortical social brain as a model for studying social behavior disorders

The zebrafish subcortical social brain as a model for studying social behavior disorders

Dose‐ and time‐dependent effects of an immune challenge on fish across biological levels

Pain and Sickness Behaviors

Contact Info

Product

Resources

About