Characterization of senescence-associated proteases in postharvest broccoli florets

Wang, Yuh Tai; Yang, Chih Yuan; Chen, Yuting; Lin, Yen; Shaw, Jei‐Fu

doi:10.1016/j.plaphy.2004.06.003

Cited by 40 publications

(32 citation statements)

References 43 publications

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“…4B). Although changes in Chl content between controls and ethylenetreated samples are rather small compared to other publications, such as Wang et al (2004), differences are significant and Chl content is lower in treated samples. Superficial color, as well as the visible aspect of florets, reflected the same results seen in Chl content (Fig.…”

Section: Chlorophyllase Expression In Hormone-treated Broccoli Floretscontrasting

confidence: 59%

Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli

Büchert

Civello

Martínez

2011

Journal of Plant Physiology

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Section: Chlorophyllase Expression In Hormone-treated Broccoli Floretscontrasting

confidence: 59%

Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli

Büchert

Civello

Martínez

2011

Journal of Plant Physiology

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“…The proteases were separated into seven endoprotease (EP) groups and classified into metalloproteases (EP1), metallo-and cysteine proteases (EP2), serine-and aspartic proteases (EP3), cysteine proteases (EP4, EP5 and EP7) and serine proteases (EP6) based on the sensitivity to classspecific protease inhibitors . Reports have confirmed that serine protease activity contributes to early stages and later stages of flower development and that cysteine and aspartic protease activities participate throughout flower senescence in broccoli (Wang et al, 2004). Among all the proteases, cysteine proteases are the most frequent and well characterized (Stephenson & Rubinstein, 1998).…”

Section: Protein Synthesis and Degradationmentioning

confidence: 94%

“…Non-proteasome proteases have been divided into exopeptidases and endopeptidases depending on the cleavage position of target proteins. Using class-specific protease inhibitors and gelatin polyacrylamide gel electrophoresis, different classes of senescence-associated endopeptidases have been partially characterized in broccoli florets (Wang et al, 2004). The proteases were separated into seven endoprotease (EP) groups and classified into metalloproteases (EP1), metallo-and cysteine proteases (EP2), serine-and aspartic proteases (EP3), cysteine proteases (EP4, EP5 and EP7) and serine proteases (EP6) based on the sensitivity to classspecific protease inhibitors .…”

Section: Protein Synthesis and Degradationmentioning

confidence: 99%

Flower Senescence-Strategies and Some Associated Events

Shahri

Tahir

2011

Bot. Rev.

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Different strategies of petal senescence and some important events associated with it have been discussed. On the basis of sensitivity to ethylene and associated symptoms of senescence, petal senescence has been classified into five different classes; besides changes in membrane permeability, autophagy and involvement of VPEs (Vacuolar processing enzymes), degradation of nucleic acids, protein turn over and remobilization of essential nutrients during petal senescence have been discussed. Nucleus appears to play a central role in administrating the execution of the events associated with petal senescence. Protein turn over appears to be an important factor governing petal senescence in both ethylene-sensitive and ethylene-insensitive flower systems and that the loss of membrane integrity, vacuolar autophagy and remobilization of essential nutrients being its important consequences. Autophagy seems to be a main process responsible for cell dismantling and remobilization of macromolecules besides final disintegration of nucleus. A large number of senescence-associated genes have been found to be differentially expressed during petal senescence. On the basis of the available literature, a schematic model representing some important events associated with petal senescence has been constructed. The review recommends that more elaborate work is required at cellular and organelle level to understand the ethylene-independent pathway and its execution in both ethylene-sensitive and ethylene-insensitive flower systems. It also recommends that ethylene sensitivity should not be generally assigned to plants at the family level on the basis of response of a few species in a family.Résumés des stratégies différentes de pétale senescence et certains événements importants associés ont été discutés. Sur la base d'une sensibilité d'éthylène et les symptômes associés de sénescence, pétale senescence ont été classé en cinq différentes classes ; outre les modifications dans la perméabilité de la membrane, Autophagie et l'implication des VPEs (Vacuolar traitement des enzymes), la dégradation des acides nucléiques, protéines tour sur re de nutriments essentiels au cours de la senescence pétale ont été discutés. Noyau semble jouer un rôle central dans l'administration de l'exécution des événements associés pétale senescence. Tour de protéines sur semble être un facteur important régissant pétale senescence Bot. Rev. (2011) 77:152-184 dans les systèmes de fleur d'éthylène sensibles et éthylène-insensible et que la perte d'intégrité de la membrane, Autophagie vacuolar et re de nutriments essentiels à ses conséquences importantes. Autophagie semble être un processus principal responsable du démantèlement de la cellule et re de macromolécules outre finale désintégration du noyau. Un grand nombre de gènes associés senescence a été trouvé pour être exprimé différemment au cours de la senescence pétale. Sur la base de la documentation disponible, un modèle schematic représentant certains événe-ments importants associés pétale senescence a ét...

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“…The first four BoCPs described by Coupe et al (2003) are the dehydration responsive cysteine proteases, while BoCP5 is very similar to the aleurain-like protease. Four additional senescence-associated cysteine endoproteases (EP2, EP4, EP5 and EP7) have been described by Wang et al (2004).…”

Section: Introductionmentioning

confidence: 99%

2-D zymographic analysis of Broccoli (Brassica oleracea L. var. Italica) florets proteases: Follow up of cysteine protease isotypes in the course of post-harvest senescence

Rossano

Larocca

Riccio

2011

Journal of Plant Physiology

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Characterization of senescence-associated proteases in postharvest broccoli florets

Cited by 40 publications

References 43 publications

Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli

Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli

Flower Senescence-Strategies and Some Associated Events

2-D zymographic analysis of Broccoli (Brassica oleracea L. var. Italica) florets proteases: Follow up of cysteine protease isotypes in the course of post-harvest senescence

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