Characterization of Semi-Interpenetrated Network Alginate/Gelatin Wound Dressing Crosslinked at Sol Phase

Perkasa, Dian Pribadi; Erizal, Erizal; Purwanti, Tri; Tontowi, Alva Edy

doi:10.22146/ijc.25710

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…As a result, the divalent ion Ca 2+ of cross-linker CaCl 2 displaces Na + ions and forms ionic bonds with carboxylate ions. Thus, the increasing ionic bonds might strengthen the matrix, escalate the amount of encapsulated bioactive ingredients, and result in the greatest encapsulation efficiency [26][27]. The results agreed with previous research [28], which stated that a higher pH solution would produce less protonated forms.…”

Section: ■ Results and Discussionsupporting

confidence: 87%

Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts Using Alginate: Preparation, Biological Activities, and Release

et al. 2023

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The bioactive compounds naturally present in plants have great importance due to their biological characteristics. These substances could lose their active characteristics since they are highly unstable. Microencapsulation is one of the techniques to improve stability and protect these compounds. In this work, Ruellia tuberosa L. ethanolic extracts microcapsules were prepared using a freeze-drying method by varying pH, alginate concentration, and stirring time. The encapsulation efficiency (EE), characteristics, alpha-amylase inhibition activity, and release behavior of the microcapsules were investigated. The results highlighted that the highest encapsulation efficiency for the microcapsules was obtained at pH 6, alginate concentration of 1% (w/v), and 30 min of stirring time (51.63% EE). The microcapsules mostly had spherical shapes with a mean diameter of 197.53 μm. The alpha-amylase inhibition assay from microcapsules resulted in the IC50 value of 46.66 ± 0.13 μg/mL, demonstrating high biological activity. The bioactive substances from microcapsules were released during intervals of 30–120 min at pH values of 1.2 and 7.4. Only 3.51% of the bioactive substances were released at pH 1.2 after 120 min, compared to 55.78% at pH 7.4. Overall, this work confirms the possibility of developing plant extracts with preserved biological activity using the produced microcapsules.

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Section: ■ Results and Discussionsupporting

confidence: 87%

Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts Using Alginate: Preparation, Biological Activities, and Release

et al. 2023

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“…As illustrated in Fig. 6, the FTIR spectrum of alginate indicates characteristic of O-H stretching vibration between 3,600 and 3,200 cm -1 , asymmetric COOstretching vibration at 1,604 cm -1 , symmetric COOstretching vibration at 1,415 cm -1 , and C-O-C stretching vibration at 1,032 cm -1 [30]. Compared to the FTIR spectrum ) and the symmetric COOstretching vibration (from 1,415 to 1,417 cm -1 ).…”

Section: Resultsmentioning

confidence: 92%

Response Surface Optimization of Gamma Irradiation Synthesis of Alginate-Stabilized Silver Nanoparticles Without Addition of a Hydroxyl Radical Scavenger

Perkasa¹,

Arozal²,

Kusmardi³

et al. 2022

Atom Indo.

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The use of isopropanol as a hydroxyl radical scavenger on the radiosynthesis of alginate-stabilized silver nanoparticles (AgNPs) can limit its application in nanomedicine. Meanwhile, optimum condition for gamma irradiation synthesis of alginate-stabilized AgNPs without addition of a hydroxyl radical scavenger has not been reported yet. In this study, the optimization of this process was carried out using response surface methodology (RSM) combined with Central Composite Design (CCD). The three processing conditions, i.e. radiation dose, precursor silver ion concentration, and alginate concentration were selected as decision variables to maximize two responses in terms of the conversion yield and AgNP concentration responses. The results indicated that the regression model of conversion yield and AgNP concentration fit linearly with the two-factor interaction and the linear model, respectively. The significant effect of the alginate factor on the conversion yield indicates the dual stabilizing-scavenging role of the alginate. The optimum conditions derived from CCD-RSM were obtained at a 20 kGy radiation dose, 7.78 mM precursor silver ion concentration, and 1.2 % (w/v) alginate concentration with the desirability of 0.731. The actual experimental results were 65.43% conversion yield and 480.91 ppm AgNP concentration, which were within the prediction interval at confidence of 95 %. The AgNPs under the optimum condition had a spherical shape, 97.4 % volume of size distribution at 6.50-28.21 nm, and zeta potential of -28.3 mV.

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“…Only the alginate group (ALG) showed peaks at 3300 cm −1 from the stretching peak of O–H, 1569 and 1417 cm −1 from asymmetric and symmetric stretching vibrations of COO, respectively [ 35 ]. In addition, the C–C vibration of the M block and C-O–H stretching vibration of the G block in alginate appeared at 1089 and 1029 cm −1 , respectively [ 35 , 36 ]. Pure gelatin showed amide acids at 1637, 1533, and 1216 cm −1 , indicating amide 1 (C = O and C-N stretching vibrations), amide 2 (N–H bending vibration and C-N stretching vibration), and amide 3 (N–H bending vibration and C-N stretching vibration), respectively.…”

Section: Resultsmentioning

confidence: 99%

NK cells encapsulated in micro/macropore-forming hydrogels via 3D bioprinting for tumor immunotherapy

Kim

et al. 2023

Biomater Res

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Background Patients face a serious threat if a solid tumor leaves behind partial residuals or cannot be completely removed after surgical resection. Immunotherapy has attracted attention as a method to prevent this condition. However, the conventional immunotherapy method targeting solid tumors, that is, intravenous injection, has limitations in homing in on the tumor and in vivo expansion and has not shown effective clinical results. Method To overcome these limitations, NK cells (Natural killer cells) were encapsulated in micro/macropore-forming hydrogels using 3D bioprinting to target solid tumors. Sodium alginate and gelatin were used to prepare micro-macroporous hydrogels. The gelatin contained in the alginate hydrogel was removed because of the thermal sensitivity of the gelatin, which can generate interconnected micropores where the gelatin was released. Therefore, macropores can be formed through bioprinting and micropores can be formed using thermally sensitive gelatin to make macroporous hydrogels. Results It was confirmed that intentionally formed micropores could help NK cells to aggregate easily, which enhances cell viability, lysis activity, and cytokine release. Macropores can be formed using 3D bioprinting, which enables NK cells to receive the essential elements. We also characterized the functionality of NK 92 and zEGFR-CAR-NK cells in the pore-forming hydrogel. The antitumor effects on leukemia and solid tumors were investigated using an in vitro model. Conclusion We demonstrated that the hydrogel encapsulating NK cells created an appropriate micro–macro environment for clinical applications of NK cell therapy for both leukemia and solid tumors via 3D bioprinting. 3D bioprinting makes macro-scale clinical applications possible, and the automatic process shows potential for development as an off-the-shelf immunotherapy product. This immunotherapy system could provide a clinical option for preventing tumor relapse and metastasis after tumor resection. Graphical Abstract Micro/macropore-forming hydrogel with NK cells fabricated by 3D bioprinting and implanted into the tumor site.

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Characterization of Semi-Interpenetrated Network Alginate/Gelatin Wound Dressing Crosslinked at Sol Phase

Cited by 6 publications

References 22 publications

Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts Using Alginate: Preparation, Biological Activities, and Release

Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts Using Alginate: Preparation, Biological Activities, and Release

Response Surface Optimization of Gamma Irradiation Synthesis of Alginate-Stabilized Silver Nanoparticles Without Addition of a Hydroxyl Radical Scavenger

NK cells encapsulated in micro/macropore-forming hydrogels via 3D bioprinting for tumor immunotherapy

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