Abstract:measurements, stopped-flow fluorescence and ITC. We are using pyrene labeled DNA, IkBa, or NF-kb to study the fluorescence changes occurring during the enhanced dissociation process. Our results show that IkBa increases the dissociation rate of the DNA from the NF-kB complex in a concentration-dependent manner and with high efficiency. We also repeated the experiments using different DNA chains and different mutants of IkBa. We are also studying IkBb, which appears to stabilize the NF-kB/DNA interaction. Both … Show more
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