Characterization of S1/mung-bean-type nuclease activity in plant cell suspensions

Grafi, Gideon; Meller, Erna; Sher, Naamit; Sela, Ilan

doi:10.1016/0168-9452(91)90261-6

Cited by 5 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, EDTA was revealed to inhibit PLBs micropropagation. It has been reported that free type of EDTA in liquid medium inhibits pyruvate dehy drogenase action in TCA cycle [12] and S1/mung-beantype nuclease activity of plant cells [13]. Therefore, it is possible that inhibitory effect of EDTA on PLBs micropropagation is attributed to their inhibitory effect on these enzyme systems.…”

Section: Discussionmentioning

confidence: 95%

Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.

Kishi

Kagami

Takagi

1997

Plant Biotechnology

View full text Add to dashboard Cite

Liquid media suitable for the induction and micropropagation of protocorm-like bodies (PLBs) in Vandofinetia Nara 'Yumika Pink' were developed by modifying MS basal medium. The best medium obtained for PLBs induction was one-fourth strength MS medium containing 30 g/l sucrose and for PLBs micropropagation was one-fourth strength MS medium containing 10 g/l sucrose in which FeEDTA was replaced by 6.95 mg/l FeSO47H2O. Usefulness of the latter medium was confirmed in other monopodial orchids such as Renanetia, Darwinara, Ascocentrum and Ascofinetia. After transfer onto 2 g/l Hyponex (N: P:K =10:30:20) medium containing 2 g/l proteose peptone and 20 g/l sucrose for 2 months, the PLBs (both those regenerating and those not regenerating plantlets) were subcultured into MS medium containing 30 g/ l sucrose at 2 months intervals for 4 months. More than 10,000 plantlets ready for transplanting to pots were obtained efficiently from one floral bud within a year for each orchid cultivar. These results suggest that the methodology could be used on a commercial scale for micropropagation of a wide range of monopodial orchids.

show abstract

Section: Discussionmentioning

confidence: 95%

Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.

Kishi

Kagami

Takagi

1997

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…Although the reaction condition favored the activity of singlestranded DNA endonucleases (low pH and Zn 2 ÷ ions as cofactors), we wanted to better characterize the enzyme activity in mung bean extracts by analyzing the cleavage sites. The activity of single-stranded DNA endonucleases toward supercoiled DNA has been described previously; the nuclease cleaves supercoiled DNA at specific sites and often at A + T-rich regions [2,13,18,19,29]. An analysis of the linear form plasmid DNA generated by the root extract showed (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…[ 13,22,29]. Some of these enzymes have proved to be useful tools in molecular biology research [8 ], but their biological significance is unknown.…”

Section: Introductionmentioning

confidence: 99%

Activity of single-stranded DNA endonucleases in mung bean is associated with cell division

Grafi

1995

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

A single-strand-specific endonuclease from mung bean sprouts is widely used in molecular biology. However, the biological role of this enzyme is unknown. We studied the spatial and temporal activity of single-stranded DNA endonucleases in mung bean seedling by following enzyme activity that linearizes supercoiled plasmid DNA, a characteristic of this type of enzyme. The formation of a linear molecule from supercoiled DNA was found to occur in two distinguishable steps. The first, which involves introducing a nick into the supercoiled DNA and relaxing it, is very rapid and complete within a few seconds. The second step of cleaving the opposite strand to generate a unit-length linear duplex DNA is a relatively slow process. Analysis of the DNA cleavage sites showed the nuclease preferentially cuts supercoiled DNA at an AT-rich region. Varying levels of nuclease activity could be detected in different tissues of the mung bean seedling. The highest activity was in the root tip and was correlated with histone H1 kinase activity. This implies a link between nuclease activity and cell division. Induction of cell division in mung bean hypocotyls with auxin promoted formation of root primordia and considerably increased the activity of single-stranded DNA endonucleases. The nuclease activity and histone H1 kinase activity were reduced in mung bean cuttings treated with hydroxyurea, but not in cuttings treated with oryzalin. The potential function of single-stranded DNA endonucleases is discussed.

show abstract

Endonucleases

Sugiyama¹,

Ito²,

Aoyagi³

et al. 2000

Programmed Cell Death in Higher Plants

View full text Add to dashboard Cite

Characterization of S1/mung-bean-type nuclease activity in plant cell suspensions

Cited by 5 publications

References 31 publications

Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.

Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.

Activity of single-stranded DNA endonucleases in mung bean is associated with cell division

Endonucleases

Contact Info

Product

Resources

About