Characterization of Ribonucleic Acid from Visna Virus

The genome of equine infectious anemia virus, a nononcogenic retrovirus, has been characterized by velocity sedimentation, electrophoresis in polyacrylamide gels, buoyant density in Cs2SO4, and susceptibility to nuclease digestion. The nucleic acid of purified virus was resolved by sedimentation analysis into a fastsedimenting genome component, which comprises about two-thirds of the virion RNA, and a slow-sedimenting RNA, which is probably comprised of host-derived tRNA and a trace amount of 5S RNA. The fast-sedimenting RNA had a sedimentation coefficient of 62S and a molecular weight of 5.4 x 10' to 5.6 x 106,

Section: Resultssupporting

confidence: 90%

Characterization of RNA from equine infectious anemia virus

Cheevers¹,

Archer²,

Crawford³

1977

“…= 81.5 + 16.6(log M) + 0.41(%G+C) -0.72% formamide where T,,, is the melting temperature and M is the molarity of monovalent salts (20). For visna virus DNA, %G+C equals 51% (25). The extensive washes in lx SSC-0.1% SDS were at temperatures equivalent to the stringencies of the 35°C hybridization conditions with different formamide concentrations; specifically, the temperatures of the washes were as follows: for 20% formamide, 36°C; for 30% formamide, 44°C; for 40% formamide, 51°C; and for 50% formamide, 58°C.…”

Section: Methodsmentioning

confidence: 99%

Genetic variation among lentiviruses: homology between visna virus and caprine arthritis-encephalitis virus is confined to the 5' gag-pol region and a small portion of the env gene

Pyper¹,

Clements²,

Molineaux³

et al. 1984

Visna virus of sheep and arthritis-encephalitis virus of goats are serologically related but genetically distinct retroviruses which cause slowly progressive diseases in their natural hosts. To localize homologous regions of the DNAs of these two viruses, we constructed a physical map of caprine arthritis-encephalitis virus DNA and aligned it with the viral RNA. Cloned probes of visna virus DNA were then used to localize regions of homology with the caprine arthritis-encephalitis virus DNA. These studies showed homology in the 5' region of the genome encompassing U5 and the gag and pol genes and also in a small region in the env gene. These findings correlate with biological data suggesting that the regions of the DNA which are homologous may be responsible for virus group characteristics such as the closely related virus core antigens. Regions which did not show homology such as large sections in the env gene may represent unique sequences which control highly strainspecific characteristics such as the neutralization antigen and specific cell tropisms.

“…Visna virus is the agent of a slow demyelinating disease of the central nervous system of sheep (21). The resemblances between Visna virus and the RNA tumor viruses are numerous and include the presence of 60 to 70S RNA which can be dissociated into 30 to 40S subunits (2,3,10,16). This RNA has an important secondary structure and is rather extensively "nicked" when extracted from particles harvested at long time intervals (3).…”

mentioning

confidence: 99%

Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

Brahic

Vigne

1975

and ethylenediacrylate were from Eastman Organic Chemicals.Acrylami'de and bisacrylamide were from Biorad.RNase-free sucrose, used for RNA work, was purchased from Schwarz-Mann.[3H ]uridine (20 Ci/mM) and carrier-free sP were from CEA, France. All other chemicals were obtained as reagent grade.TNE buffer refers to the following solution: 0.01 M Tris-hydrochloride (pH 7.5), 0.1 M NaCl, and 0.001 M EDTA. TNE-SDS buffer refers to the same solution containing 0.5% sodium dodecyl sulfate (SDS). For the RNA work, the buffers and plastic pieces of equipment were treated with diethylpyrocarbonate 1222 on July 7, 2020 by guest