Characterization of retinoic acid-inducible gene-I (RIG-I) expression corresponding to viral infection and UVB in human keratinocytes

Kimura, Kazuyuki; Matsuzaki, Yasushi; Nishikawa, Yohei; Kitamura, Hideo; Akasaka, Eijiro; Rokunohe, Daiki; Nakano, Hajime; Imaizumi, Tadaatsu; Satoh, Kei; Sawamura, Daisuke

doi:10.1016/j.jdermsci.2012.02.006

Cited by 19 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro, the transcriptional levels of AjRIG-I in Japanese eel liver cells were significantly enhanced at 3 h, 6 h and 24 h after treatment with poly I:C (Fig. 4) and this modulation of AjRIG-I expression pattern was coincident with the reports in various cells, such as human skin keratinocyte [37], bat kidney cells [38], grass carp kidney (CIK) cells [24], and the pacific oyster hemocytes [39]. Furthermore, virus from grass carp and channel catfish have been demonstrated to strong induce the RIG-I gene express in both CIK cells and channel catfish ovarian cells [23,24].…”

Section: Discussionsupporting

confidence: 62%

Identification of a retinoic acid-inducible gene I from Japanese eel (Anguilla japonica) and expression analysis in vivo and in vitro

Feng

Guo

Lin

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 62%

Identification of a retinoic acid-inducible gene I from Japanese eel (Anguilla japonica) and expression analysis in vivo and in vitro

Feng

Guo

Lin

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…findings suggest that this synergistic induction may be due to the increase in TLR3 expression and the function induced by IFN-γ. Poly (I:C) is also recognized by MDA5 and RIG-I (Kalali et al, 2008;Kimura et al, 2012), but we here have demonstrated that this synergistic induction is, at least in part, mediated by TLR3.…”

Section: Discussionmentioning

confidence: 50%

Interferon-Gamma Enhances TLR3 Expression and Anti-Viral Activity in Keratinocytes

Kajita

Morizane

Takiguchi

et al. 2015

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Toll-like receptors (TLRs) recognize specific microbial products in the innate immune response. TLR3, a double-stranded RNA sensor, is thought to have an important role in viral infections, but the regulation of TLR3 expression and its function in keratinocytes are not fully understood. Here we show the Th1 cytokine IFN-γ increased the TLR3 expression via STAT1 in cultured normal human epidermal keratinocytes (NHEKs). Co-stimulation with IFN-γ and the TLR3 ligand poly (I:C) synergistically increased the expression of IFN-β, IL-6, IL-8, and human β-defensin-2 in NHEKs compared with poly (I:C) or IFN-γ alone. These synergistic inductions were significantly inhibited by an endosomal acidification inhibitor, chloroquine, and by TLR3 siRNA. Co-stimulation with IFN-γ and poly (I:C) also significantly enhanced the anti-viral activity against herpes simplex virus type-1 in NHEKs compared with poly (I:C) or IFN-γ alone. In addition to the in vitro findings, an immunohistochemical analysis revealed IFN-γ-positive cells surrounding herpetic vesicles. These findings indicate that IFN-γ might contribute to the innate immune response to cutaneous viral infections by enhancing TLR3 expression and function in keratinocytes.

show abstract

“…IRF-3 is generally thought to be the more important 39,121,122 , although it is likely that IRF-7 may make significant contributions to some of the effects of poly(I:C) and other inflammatory stimulants 123,124 . The induction of IFN-b depends on IRF-3 41 as does the induction of retinoic acid-inducible gene-1 (RIG-1) 125 , while IRF-3 modulates the induction of MCP-1 produced by poly(I:C) 126 and may thus have contributed to the increased concentrations of this chemokine we have described after poly(I:C) administration 26 .…”

Section: Discussionmentioning

confidence: 91%

Prenatal activation of maternal TLR3 receptors by viral-mimetic poly(I:C) modifies GluN2B expression in embryos and sonic hedgehog in offspring in the absence of kynurenine pathway activation

Khalil

Forrest

Pisar

et al. 2013

Immunopharmacology and Immunotoxicology

View full text Add to dashboard Cite

Activation of the immune system during pregnancy is believed to lead to psychiatric and neurological disorders in the offspring, but the molecular changes responsible are unknown. Polyinosinic:polycytidylic acid (poly(I:C)) is a viral-mimetic double-stranded RNA complex which activates Toll-Like-Receptor-3 and can activate the metabolism of tryptophan through the oxidative kynurenine pathway to compounds that modulate activity of glutamate receptors. The aim was to determine whether prenatal administration of poly(I:C) affects the expression of neurodevelopmental proteins in the offspring and whether such effects were mediated via the kynurenine pathway. Pregnant rats were treated with poly(I:C) during late gestation and the offspring were allowed to develop to postnatal day 21 (P21). Immunoblotting of the brains at P21 showed decreased expression of sonic hedgehog, a key protein in dopaminergic neuronal maturation. Expression of α-synuclein was decreased, while tyrosine hydroxylase was increased. Disrupted in Schizophrenia-1 (DISC-1) and 5-HT2C receptor levels were unaffected, as were the dependence receptors Unc5H1, Unc5H3 and Deleted in Colorectal Cancer (DCC), the inflammation-related transcription factor NFkB and the inducible oxidative enzyme cyclo-oxygenase-2 (COX-2). An examination of embryo brains 5 h after maternal poly(I:C) showed increased expression of GluN2B, with reduced doublecortin and DCC but no change in NFkB. Despite altered protein expression, there were no changes in the kynurenine pathway. The results show that maternal exposure to poly(I:C) alters the expression of proteins in the embryos and offspring which may affect the development of dopaminergic function. The oxidation of tryptophan along the kynurenine pathway is not involved in these effects.

show abstract

Characterization of retinoic acid-inducible gene-I (RIG-I) expression corresponding to viral infection and UVB in human keratinocytes

Cited by 19 publications

References 33 publications

Identification of a retinoic acid-inducible gene I from Japanese eel (Anguilla japonica) and expression analysis in vivo and in vitro

Identification of a retinoic acid-inducible gene I from Japanese eel (Anguilla japonica) and expression analysis in vivo and in vitro

Interferon-Gamma Enhances TLR3 Expression and Anti-Viral Activity in Keratinocytes

Prenatal activation of maternal TLR3 receptors by viral-mimetic poly(I:C) modifies GluN2B expression in embryos and sonic hedgehog in offspring in the absence of kynurenine pathway activation

Contact Info

Product

Resources

About