Characterization of Red Raspberry Cultivars and Selections Using Isoenzyme Analysis

Cousineau, J.; Anderson, Angela K.; Daubeny, Hugh A.; Donnelly, Danielle J.

doi:10.21273/hortsci.28.12.1185

Cited by 12 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…X neglectus Peck) with five enzymes out of 14 yielding consistent and polymorphic fragment patterns (Cousineau & Donnelly, 1989) . Unfortunately only 14 out of the 20 cultivars investigated could be uniquely characterized with this method .…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness

Nybom

Hall²

1991

Euphytica

View full text Add to dashboard Cite

Minisatellite DNA from 13 Rubus cultivars, including five raspberries, two R . flagellaris derivatives, three R . ursinus derivatives, and the three raspberry-blackberry hybrids Boysen, Logan Thornless and Young, was hybridized with the M13 probe yielding cultivar-specific DNA fragment profiles . Estimates of similarity, assessed from the fragment profiles, showed a strong association with the degree of genetic relatedness among the cultivars . Boysen was shown to be closely related to Austin Thornless, Logan and Young, and is thus thought to be of similar origin to Young, that is Austin Thornless X Phenomenal . While genetic relatedness of Creston with Lloyd George was suggested, Lloyd George parentage of Creston cannot be confirmed from the data obtained .

show abstract

Section: Resultsmentioning

confidence: 95%

“…Isozyme analysis has likewise only been moderately successful in distinguishing cultivars (Cousineau & Donnelly, 1989) . However, a set of ten Rubus cultivars could be uniquely characterized by applying DNA minisatellite analysis (Nybom et al ., 1989) .…”

Section: Introductionmentioning

confidence: 99%

Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness

Nybom

Hall²

1991

Euphytica

View full text Add to dashboard Cite

show abstract

“…(Marquard and Chan 1995); raspberry, Rubus spp. (Cousineau and Donnelly 1989); guayule, Parthenium argentatum Gray (Estilai et al 1990); bananas, Musa spp. (Bhat et al 1992); tulip, Tulipa spp.…”

mentioning

confidence: 99%

Isozyme electrophoretic characterization of 29 related cultivars of lily (Lilium spp.)

et al. 2005

View full text Add to dashboard Cite

Isozyme banding patterns (IBPs) were studied for cultivars of lily (Lilium spp.) by means of horizontal starch-gel electrophoresis (SGE). An array of continuous histidine-citrate buffer systems at eight ranges of pH and four extraction buffers were tested. On the basis of this survey, the extraction buffer two (Eb-2) and the buffer system E at pH 7.7 were found to be suitable for detection of lily isozymes. EC 5.3.1.9) and 6-phosphogluconate dehydrogenase (PGD; EC 1.1.1.44) were assayed. In total 29 cultivars were tested in this study: nine were analysed for all eight enzyme systems, 16 cultivars for seven systems, three for six, and one for five enzyme systems. Some IBP were identified as section-specific biochemical markers. Eight enzymes systems were analysed by constructing a dendogram using the unweighted pair group method, arithmetic average (UPGMA) cluster analysis. The analysis indicated that the lily cultivars could be separated from other Lilium species, except for two L. x formonlogi cultivars: ÔHakubaÕ and ÔHakukoÕ which could not be distinguished from each other by the isozyme patterns assayed here. This study shows that isozymes can provide useful biochemical markers for lily cultivar identification and to estimate the phylogenetic relationships among those cultivars.

show abstract

“…Isoenzyme variability was used in genetic analysis of diploid Rubus species (Cousineau and Donnelly 1992) and was widely used for identifying raspberry varieties (Cousineau et al 1993;Pandolfi et al 1993). Electrophoresis of soluble proteins was used for distinguishing raspberry and blackberry hybrids (Lopez-Medina et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Population evaluation in crop wild relatives for in situ conservation: a case study for raspberry Rubus idaeus L. in the Leningrad region, Russia

Ryabova

2007

Genet Resour Crop Evol

View full text Add to dashboard Cite

Many crop wild relatives are usually widely distributed species, and in this relation a question arises about selecting populations of special importance for conservation. Using Rubus idaeus as an example, we propose a compound selection of wild populations for in situ conservation. Twelve raspberry populations in the Leningrad region have been evaluated from the point of view of their significance for in situ conservation. To exclude the run-wild forms from the evaluation process, we tried to identify them applying a mathematical model developed by statusmetry method. The constructed model is based on morphological features of leaves of primocanes and helps to distinguish the wild forms from the cultivated and run-wild ones. The wild raspberry populations were screened for plants with features useful for cultivated raspberry improvement such as waxy cover presence, yellow fruiting plants, spinelessness, fruiting primocanes and others. The diversity of wild raspberry populations was assessed by variation of morphological features, presence of botanical varieties and polymorphism of seed storage proteins. The results of viability status assessment for the chosen populations reflect the perspectives of their development and permit to determine the strategy and measures for optimal conservation.Keywords Crop wild relatives AE Evaluation AE In situ conservation AE Rubus idaeus AE Storage protein polymorphism AE Wild raspberry From the point of view of evolution and genetics, crop wild relatives (CWR) are the species of native flora that either belong to the same genus with cultivar, or participated in the cultivars evolution of other genus complexes. Together with the cultivated plants, CWR compose the plant genetic resources of any country that should be preserved for the future (Vavilov 1931;Brezhnev and Korovina 1981;Korovina 1986).At present the in situ conservation method is comprising of a number of measures taken to preserve the species and ecosystems in their native habitats, including maintenance and recovery of the viable populations of the target species (Maxted et al. 1997). However, many CWR are widely distributed species, and in this relation a question arises about selecting populations of special importance for conservation. Using raspberry Rubus idaeus (Rubus subgenera Idaeobatus (Focke) Focke sect. Batidaea Dumort. ser. Euidaei Focke) as an example, we propose a compound selection of valuable wild populations for in situ conservation.

show abstract

Characterization of Red Raspberry Cultivars and Selections Using Isoenzyme Analysis

Cited by 12 publications

References 5 publications

Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness

Minisatellite DNA ‘fingerprints’ can distinguish Rubus cultivars and estimate their degree of relatedness

Isozyme electrophoretic characterization of 29 related cultivars of lily (Lilium spp.)

Population evaluation in crop wild relatives for in situ conservation: a case study for raspberry Rubus idaeus L. in the Leningrad region, Russia

Contact Info

Product

Resources

About