Characterization of reactive nitrogen species in allergic asthma

Zuo, Li; Koozechian, M; Chen, Lauren L.

doi:10.1016/j.anai.2013.10.007

Cited by 45 publications

(32 citation statements)

References 66 publications

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“…Exhaled NO is produced from arginine and is well known for its association with asthma and asthma severity. 17,18 Low arginine levels are associated with impaired smooth muscle relation in vitro. 19 In addition, asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of NO synthase.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic profiling of asthma and chronic obstructive pulmonary disease: A pilot study differentiating diseases

Adamko

Nair

Mayers

et al. 2015

Journal of Allergy and Clinical Immunology

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Section: Discussionmentioning

confidence: 99%

Metabolomic profiling of asthma and chronic obstructive pulmonary disease: A pilot study differentiating diseases

Adamko

Nair

Mayers

et al. 2015

Journal of Allergy and Clinical Immunology

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“…It has been proposed that damage to DNA and tissue induced by ROS and NO-derived reactive nitrogen species may contribute to increased mutation rates, genome instability, apoptosis and the associated tissue regeneration and cell proliferation, all of which can drive allergic diseases such as asthma [4,5,6,7,8]. Eosinophils play a major role in ROS generation in the asthmatic airways [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Dilek

Özkaya²,

Koçyiğit³

et al. 2015

Int Arch Allergy Immunol

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Background: There is ample knowledge reported in the literature about the role of oxidative stress in asthma pathogenesis. It is also known that the interaction of reactive oxygen species with DNA may result in DNA strand breaks. The aim of this study was to investigate if montelukast monotherapy affects oxidative stress and DNA damage parameters in a population of pediatric asthma patients. Methods: Group I consisted of 31 newly diagnosed asthmatic patients not taking any medication, and group II consisted of 32 patients who had been treated with montelukast for at least 6 months. Forty healthy control subjects were also enrolled in the study. Plasma total oxidant status (TOS) and total antioxidant status (TAS) were measured to assess oxidative stress. DNA damage was assessed by means of alkaline comet assay. Results: The patients in both group I and group II had statistically significant higher plasma TOS (13.1 ± 4 and 11.1 ± 4.1 μmol H₂O₂ equivalent/liter, respectively) and low TAS levels (1.4 ± 0.5 and 1.5 ± 0.5 mmol Trolox equivalent/liter, respectively) compared with the control group (TOS: 6.3 ± 3.5 μmol H₂O₂ equivalent/liter and TAS: 2.7 ± 0.6 mmol Trolox equivalent/liter; p < 0.05). DNA damage was 18.2 ± 1.0 arbitrary units (a.u.) in group I, 16.7 ± 8.2 a.u. in group II and 13.7 ± 3.4 a.u. in the control group. There were statistically significant differences only between group I and the control group (p < 0.05). Conclusions: According to the findings, montelukast therapy makes only minimal but not statistically significant improvement in all TOS, TAS and DNA damage parameters.

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“…On the one hand, it inhibits chemokine production in mast cells [21,38] , while on the other hand it induces vasodilatation, vasopermeation, and leukocyte-endothelial adherence [38] . We think that the proinflammatory properties of NO outweigh its anti-inflammatory properties in CSU, as in the case of asthma [39] .…”

Section: Discussionmentioning

confidence: 99%

Elevated Nitrosative Stress in Children with Chronic Spontaneous Urticaria

Dilek

Özçeker²,

Özkaya³

et al. 2017

Int Arch Allergy Immunol

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Background: Chronic spontaneous urticaria (CSU) is an idiopathic condition that seriously affects quality of life. It is well known that oxidative stress and nitrosative stress (NS) are generally involved in many chronic inflammatory diseases. This study aimed to evaluate the possible role of NS in the pathogenesis of CSU. Methods: Thirty-two children with CSU and 22 healthy control subjects were enrolled in the study. Demographic and clinical features were defined, and disease activity was quantified using the urticaria activity score (UAS). Serum NS was assessed by the plasma levels of total nitric oxide (NOx) metabolites and nitrite and nitrate measurements using a Griess method-based commercial kit. Results: Plasma NOx levels were 82.5 ± 11.3 µmol/L in the CSU group and 50.9 ± 9.4 µmol/L in the control group. The difference was statistically significant (p < 0.001). CSU patients also had higher plasma nitrite levels than controls (53.3 ± 13.8 vs. 30.2 ± 10.1 µmol/L, respectively, p < 0.001). The median values of plasma nitrate were 27.5 µmol/L (IQR 19.1-35.5) in CSU patients and 20.9 µmol/L (IQR 17.9-23.2) in the control group, and the difference was statistically significant (p = 0.009). In addition, plasma NOx and nitrite levels were positively correlated with the UAS (rho = 0.512, p = 0.03 and rho = 0.452, p = 0.011, respectively). Conclusion: Plasma NS is elevated and positively correlated with disease activity in children with CSU.

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Characterization of reactive nitrogen species in allergic asthma

Cited by 45 publications

References 66 publications

Metabolomic profiling of asthma and chronic obstructive pulmonary disease: A pilot study differentiating diseases

Metabolomic profiling of asthma and chronic obstructive pulmonary disease: A pilot study differentiating diseases

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Elevated Nitrosative Stress in Children with Chronic Spontaneous Urticaria

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