Characterization of rat schwannoma-schwann cell hybrids

Kreider, Barbara; Corboy, John R.; Preston, Sheryl L.; Auzzmann, Jeanine M.; DeSalvo, S; Smith, Thomas M.; Lieb, Marge; Edinburgh, Keith Jon; Pleasure, David E; McMorris, F. Arthur

doi:10.1016/0006-8993(86)90624-4

Cited by 3 publications

(4 citation statements)

References 21 publications

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“…The rat schwannoma cell line RN22 (Pfeiffer and Wechsler, 1972) and the rat Schwann-schwannoma hybrids RNS-1, RNS-2, and RNS-5 (Kreider et al, 1986) were cultured in DMEM/10% FCS.…”

Section: Materials and Methods Northern Blot Analysesmentioning

confidence: 99%

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Cohen

Yachnis

Arai

et al. 1992

J of Neuroscience Research

108

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The neu gene, which encodes a putative tyrosine kinase growth factor receptor termed p185neu, was originally identified as a dominant transforming gene in neurogliomas and schwannomas induced by transplacental treatment of rat embryos with ethylnitrosourea. The present studies were undertaken to determine the expression pattern of the neu gene in peripheral nerve. Northern blot analysis of total RNA isolated from rat sciatic nerves demonstrated prominent neu mRNA expression on postnatal days 1 and 7, with substantially lower expression up to adulthood. Immunohistochemical studies confirmed expression of p185neu by Schwann cells (SC) in developing sciatic nerve and minimal p185neu immunoreactivity in adult nerves. However, neu mRNA and p185neu protein progressively increased following sciatic nerve transection in adult animals. In addition, neu mRNA and p185neu were found in neonatal rat sciatic nerve SC and several SC-derived cell lines. In resting SC, neu mRNA was expressed at a low level, but was greatly increased by treatment with forskolin and glial growth factor. These studies demonstrate that the neu gene and its protein product, p185neu, are expressed by SC both in vivo and in vitro and suggest that p185neu plays a role in the regulation of SC proliferation or differentiation.

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“…The rat schwannoma cell line RN22 (Pfeiffer and Wechsler, 1972) and the rat Schwann-schwannoma hybrids RNS-1, RNS-2, and RNS-5 (Kreider et al, 1986) were cultured in DMEM/10% FCS.…”

Section: Materials and Methods Northern Blot Analysesmentioning

confidence: 99%

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Cohen

Yachnis

Arai

et al. 1992

J of Neuroscience Research

108

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“…After 4 days in this medium, some cultures were treated for 3 days with M N6,2'-O-dibutyryl cyclic AMP (dibutyryl cyclic AMP) or for 4 days with axolemmal fragments (250 pg of axolemmal fragment protein/dish) prepared from adult female Sprague-Dawley rat brain stems (DeVries et al, 1983;Sobue and Pleasure, 1985). The clonal Schwann cell lines RNSl and RNS5 (Kreider et al, 1986) and their RN22 Schwannoma parent (Pfeiffer and Wechsler, 1972) were grown in T-25 flasks in a medium containing hypoxanthine, aminopterin, and thymidine (Littlefield, 1964). Some flasks were treated with Mdibutyryl cyclic AMP for 3 or 6 days or M forskolin for 3 days.…”

Section: Cell Culturementioning

confidence: 99%

“…The limited numbers of cultured Schwann cells available for study, the absence of immunochemically demonstrable Po myelin glycoprotein and MBP from the cells after several days in vitro, and the trace levels of constitutive expression of Po and MBP mRNA species make analyses of the regulation of synthesis of these myelin proteins in this culture system difficult. We therefore studied several clones of rat Schwann cell-rat Schwannoma hybrids (Kreider et al, 1986) which maintain considerably greater…”

Section: 3 4mentioning

confidence: 99%

“…The principal aim of the present study was to clarify the role of cyclic AMP in regulation of Schwann cell synthesis of myelin lipids and proteins. This aim was facilitated by the observation that two clonal lines of rat Schwann cell-rat Schwannoma hybrids, which grow rapidly and which had previously been determined to express cyclic AMP-inducible activity of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) (Kreider et al, 1986), an enzyme enriched in Schwann cells and myelin (Sprinkle et al, 1983(Sprinkle et al, , 1985Yoshino et al, 1985), constitutively express myelin Po glycoprotein (PO) and PO mRNA.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Myelin P₀ Glycoprotein Synthesis in Cultured Rat Schwann Cells and Continuous Rat PNS Cell Lines

Kreider

Zeller²,

Lazzarini³

et al. 1988

Journal of Neurochemistry

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We studied the effects of agents that raise intracellular cyclic AMP on synthesis of myelin components by cultured neonatal rat sciatic nerve Schwann cells and by continuous PNS cell lines derived from the fusion of neonatal rat sciatic nerve Schwann cells with rat RN22 Schwannoma. Treatment with N6,2'-O-dibutyryl cyclic AMP (dibutyryl cyclic AMP) caused a fourfold increase in Schwann cell incorporation of 35SO4 into sulfogalactosylceramide (sulfatide), and elicited a 10- to 20-fold increase in such incorporation by the continuous PNS cell lines; a similar effect on PNS cell line sulfatide radiolabelling was obtained with forskolin. Cultured Schwann cells expressed barely detectable levels of myelin P0 glycoprotein (P0) mRNA and myelin basic protein (MBP) mRNA. Treatment of the Schwann cells with axolemmal fragments or with dibutyryl cyclic AMP did not elicit a detectable increase in the levels of these mRNAs. The PNS cell lines constitutively expressed much higher levels of P0 mRNA than did the Schwann cells, and synthesized immunochemically demonstrable P0 glycoprotein, but did not express MBP. Treatment of the PNS cell lines with dibutyryl cyclic AMP markedly reduced expression of P0 mRNA and also diminished immunoreactive P0 glycoprotein. These PNS cell lines should prove useful for further studies of the control of Schwann cell differentiation.

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Ligand Binding to the Cell‐Surface Receptor for Reovirus Type 3 Alters Schwann Cell Growth and Function

Cohen

Williams

et al. 1990

Annals of the New York Academy of Sciences

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Characterization of rat schwannoma-schwann cell hybrids

Cited by 3 publications

References 21 publications

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Regulation of Myelin P₀ Glycoprotein Synthesis in Cultured Rat Schwann Cells and Continuous Rat PNS Cell Lines

Ligand Binding to the Cell‐Surface Receptor for Reovirus Type 3 Alters Schwann Cell Growth and Function

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Characterization of rat schwannoma-schwann cell hybrids

Cited by 3 publications

References 21 publications

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Expression of theneuproto‐oncogene by schwann cells during peripheral nerve development and wallerian degeneration

Regulation of Myelin P0 Glycoprotein Synthesis in Cultured Rat Schwann Cells and Continuous Rat PNS Cell Lines

Ligand Binding to the Cell‐Surface Receptor for Reovirus Type 3 Alters Schwann Cell Growth and Function

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Regulation of Myelin P₀ Glycoprotein Synthesis in Cultured Rat Schwann Cells and Continuous Rat PNS Cell Lines