Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Laxmi, M; Bhat, Sarita G.

doi:10.1007/s13205-015-0350-1

Cited by 33 publications

(36 citation statements)

References 10 publications

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“…The Presence of peak at 1637.82 cm -1 in all the extracted samples as observed in (Fig. 5 a-h) corresponded to C=N bond 39 . The band range between 3400-3300 cm -1 indicated the presence of -OH group in the extracted pyocyanin 40 .…”

Section: Impact On Pyocyanin Pigmentsupporting

confidence: 52%

“…The band range between 3400-3300 cm -1 indicated the presence of -OH group in the extracted pyocyanin 40 . From the LC-MS analysis in positive ion mode (Fig 8ah), the peak at 211 m/z represents pyocyanin 41 and the presence of pyochelin was detected from the peak sensed at 338 m/z 42 . Punica granatum showed impact on the synthesis of phenazine pyocyanin and Pyochelin production whereas only Pyochelin production was affected in the case of Citrus reticulata, Vitis amurensis and Vitis vinifera treated cultures (Fig.…”

Section: Impact On Pyocyanin Pigmentmentioning

confidence: 99%

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Antioxidant and Quorum Quenching Activity against Pseudomonas aeruginosa SU-18 of some Edible Fruit Juices

A¹,

Nishanthini²,

Raji³

et al. 2019

J Pure Appl Microbiol

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Besides being an essential source of nutrients, the bioactive components of some fruits also help in enhancing the physiological functions by expressing its therapeutic action, acting as radical scavenger, improving digestion and healing. Here, seven edible fruits juices of Punica granatum, Citrus reticulata, Anana scomosus, Ficus carica, Vitis vinifera, Vitis amurensis and Carica papaya were utilized against biofilm forming Gram negative bacteria, Pseudomonas aeruginosa to evaluate its effect on Quorum sensing. On proving its antibacterial activity and anti-swarming motility in our earlier report, this work is extended to determine the biofilm inhibitory action of these fruit juices due to the impact on AHL (Acyl Homoserine lactone), the signaling molecule responsible for developing cell-cell communication and also on AHL mediated metabolites production. The fruit juices were evaluated for their Antioxidant activity on subjecting to TLC bioautography, DPPH and FRAP assay. AHL, Pyocyanin and Rhamnolipid were extracted from fruit juices treated Pseudomonas aeruginosa and the influence of fruit juice was identified by FT-IR and LC-MS analysis. However, AHL production was not stopped by fruit juice molecules but showed least production level in Punica granatum treated P.aeruginosa. Whereas the production of pyocyanin pigment was disturbed in Punica granatum, Citrus reticulata, Vitis amurensis and Vitis vinifera treated culture. The production of a biosurfactant called Rhamnolipid (Rha 10) was a failure in Citrus reticulata, Vitis amurensis, Vitis vinifera and Ficus carica treated P.aeruginosa. Thus the organism was restrained by the fruit juice molecules from expressing its virulence factors in spite of having no impact on AHL synthesis.

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Section: Impact On Pyocyanin Pigmentsupporting

confidence: 52%

Section: Impact On Pyocyanin Pigmentmentioning

confidence: 99%

Antioxidant and Quorum Quenching Activity against Pseudomonas aeruginosa SU-18 of some Edible Fruit Juices

A¹,

Nishanthini²,

Raji³

et al. 2019

J Pure Appl Microbiol

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“…The concentrations for cytotoxicity studies ranged from 6.25 -100 µg/mL along with the Biofilm Inhibitory Concentration at nanogram quantity (Unpublished data). The BIC was found to be much lower compared to the Minimum Inhibitory Concentration (MIC) of pyocyanin calculated for all nine pathogens which was 0.6225 µg/mL [16].…”

Section: Results and Discussion Antibiofilm Activity And Determinatimentioning

confidence: 72%

“…Pyocyanin produced by strain Pseudomonas aeruginosa (BTRY1), was extracted and characterized using various spectroscopic methods namely UV Visible spectroscopy, Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform Infra-Red spectroscopy (FTIR) [16]. The compound was found to have no hemolytic and cytotoxic activities along with a high free radical scavenging activity [16].…”

Section: Pyocyaninmentioning

confidence: 99%

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Confocal and SEM imaging to demonstrate food pathogen- biofilm biocontrol by pyocyanin from Pseudomonas aeruginosa BTRY1

Mohandas

Anju

Bhat

2016

IJB

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An assortment of redox-active phenazine compounds like pyocyanin with their characteristic blue-green colour are synthesized by Pseudomonas aeruginosa, Gram-negative opportunistic pathogens, which are also considered one of the most commercially valuable microorganisms. In this study, pyocyanin from Pseudomonas aeruginosa BTRY1 from food sample was assessed for its antibiofilm activity by micro titer plate assay against strong biofilm producers belonging to the genera Bacillus, Staphylococcus, Brevibacterium and Micrococcus. Pyocyanin inhibited biofilm activity in very minute concentrations. This was also confirmed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Both SEM and CLSM helped to visualize the biocontrol of biofilm formation by eight pathogens. The imaging and quantification by CLSM also established the impact of pyocyanin on biofilm-biocontrol mainly in the food industry.

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Pyocyanin as a safe aquaculture drug for the control of vibriosis in shrimp recirculating aquaculture system (RAS)

et al. 2022

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Characterization of pyocyanin with radical scavenging and antibiofilm properties isolated from Pseudomonas aeruginosa strain BTRY1

Cited by 33 publications

References 10 publications

Antioxidant and Quorum Quenching Activity against Pseudomonas aeruginosa SU-18 of some Edible Fruit Juices

Antioxidant and Quorum Quenching Activity against Pseudomonas aeruginosa SU-18 of some Edible Fruit Juices

Confocal and SEM imaging to demonstrate food pathogen- biofilm biocontrol by pyocyanin from Pseudomonas aeruginosa BTRY1

Pyocyanin as a safe aquaculture drug for the control of vibriosis in shrimp recirculating aquaculture system (RAS)

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