Characterization of Protein Hydrolysates from Fish Discards and By-Products from the North-West Spain Fishing Fleet as Potential Sources of Bioactive Peptides

Abstract

Fish discards and by-products can be transformed into high value-added products such as fish protein hydrolysates (FPH) containing bioactive peptides. Protein hydrolysates were prepared from different parts (whole fish, skin and head) of several discarded species of the North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture and ash contents lower than 10% and 15%, respectively. The fat content of FPH varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The amino acids profiles of FPH are quite similar and the most abundant amino acids were glutamic and aspartic acids. All FPH exhibited antioxidant activity and those obtained from Atlantic horse mackerel heads presented the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and Cu2+ chelating activity. On the other hand, hydrolysates from gurnard heads showed the highest ABTS radical scavenging activity and Fe2+ chelating activity. In what concerns the α-amylase inhibitory activity, the IC50 values recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and whole Atlantic horse mackerel, respectively. α-Glucosidase inhibitory activity of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme (ACE) inhibitory activity. Considering the biological activities, these FPH are potential natural additives for functional foods or nutraceuticals.

Characterization of Gelatin and Hydrolysates from Valorization of Farmed Salmon Skin By-Products

“…The hydrolysates still contain some collagenous material, as shown by the presence of proline and hydroxyproline (almost exclusive to collagen). The contribution of other proteins raises the ratio of essential aminoacids to 41.3%, comparable to hydrolysates prepared from other species [ 44 , 45 , 46 ], with digestibilities of up to 85.9%. Furthermore, in vitro antihypertensive activity in the Alcalase hydrolysate CH2 (82.3% inhibition; 71.2 µg protein/mL-IC 50 ) compares favorably with hydrolysates from salmon heads (71.9% inhibition; 478.5 µg protein/mL-IC 50 ) and frames and trimmings (87.0% inhibition; 653.7 µg protein/mL-IC 50 ) [ 20 ].…”

Proteases Production and Chitin Preparation from the Liquid Fermentation of Chitinous Fishery By-Products by Paenibacillus elgii

“…The hydrolysates of SPP, SHP and SBP exhibited higher α-glucosidase inhibitory activity than their initial materials, while the hydrolysates from SMP, FMP, and FHP showed lower results. The difference in the α-glucosidase inhibitory activity among the hydrolysates and starting materials may relate to their amino acid compositions and sizes [ 38 ]. In this study, the SBP hydrolysate exhibited the highest α-glucosidase inhibitory activity (37.39%).…”

Functional yogurt fortified with fish collagen‐derived bioactive peptides: Antioxidant capacity, ACE and DPP‐IV inhibitory

“…In a research effort by Henriques et al. (2021), protein hydrolysates from fish discards and by‐products were analyzed for potential antioxidant activity. The results of the study indicated that the DPPH radical scavenging activity of the hydro lysates was dose‐dependent like that we obtained from the present study.…”