Characterization of polyphenol compounds from the roots and stems of Parthenocissus laetevirens by high‐performance liquid chromatography/tandem mass spectrometry

Abstract

A facile method based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC/(-)ESI-MSn) has been established for the analyses of polyphenol compounds in the root and stems of Parthenocissus laetevirens. Two characteristic fragments [C3O2 (68 Da) and C2H2O (42 Da)] were utilized for the structural identification of polyphenols. Based on the reference standards, the fragment C3O2 was presented when the compound possessed a 2,3-dihydro-1H-indene-4, 6-diol moiety. Meanwhile, the C2H2O fragment (42 Da) yielded from the resorcinol ring was confirmed by resveratrol and three synthesized compounds identified as (E)-5-styrylbenzene-1,3-diol, (E)-4-styrylphenol and (E)-4-(3,4,5-trimethoxystyryl)phenol. FTICR-MSn was performed to further confirm the structures of the fragments. Overall, 15 polyphenol compounds were characterized. Three polyphenol compounds were initially and tentatively characterized from P. laetevirens for the first time, and one was proposed as a novel compound. Furthermore, a pair of stereoisomers was readily distinguished by breakdown curves, and the trans-, cis-isomers could be identified by HPLC/DAD-UV spectra.

Characterization and Discrimination of Ancient Grains: A Metabolomics Approach

“…Since these metabolites are commonly detected by GC-MS [17] or HPLC-UV [18] techniques, MS/MS spectra were not available in the online database. Thus, we tentatively identified them checking the exact mass (mass error less than 1.7 ppm), the match of experimental and theoretical isotope pattern in terms of spacing and relative intensities, and the most abundant fragment ion [M − C 2 H 2 O]¯ yielded from the resorcinol ring, resulting from the neutral loss of 42 Da (see Figure S1) [19]. …”

Screening and quantitative analysis of antioxidants in the fruits of Livistona chinensis R. Br using HPLC-DAD–ESI/MS coupled with pre-column DPPH assay

“…HPLC-MS n has been confirmed to be an ideal tool for analyzing the natural plant secondary metabolites, owing to that it can provide affluent multistage fragment information for compounds with collision-induced dissociation (Chen, He, Mao, Sun, & Pan, 2009;Chen, Li, Sun, Pan, & Schlunegger, 2008a;Sun, Wu, & Pan, 2009;Sun, Zhu, Hu, Wang, & Pan, 2010). Therefore, in our study, high performance liquid chromatography-photo diode array detectionelectrospray ionization tandem mass spectrometry (HPLC-DAD/ ESI-MS n ) technique was employed to identification of the antioxidative components in L. chinensis fruits.…”

Distribution of metabolites in galled and non-galled leaves of Clusia lanceolata and its antioxidant activity

“…LC-ESI-MS/MS has been confirmed to be an ideal tool to analyse secondary metabolites from plants, owing to the fact that it can provide affluent multistage fragment information of compounds by collision-induced dissociation (Chen et al, 2009). Therefore, in our study, the high performance liquid chromatography with diode array detection and electrospray ionization ion trap mass spectrometry (HPLC-DAD-ESI-MS/MS) technique was employed for the identification of flavones in C. lanceolata leaves ( Table 2).…”