Characterization of pneumococcal specific antibodies in healthy unvaccinated adults

Coughlin, Richard T.; White, A; Anderson, Carol-Gay; Carlone, George M.; Klein, David L.; Treanor, John J.

doi:10.1016/s0264-410x(98)00139-x

Cited by 55 publications

(36 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As reported elsewhere (13,26), we observed that pre-vaccination levels of antibodies to serotypes 1, 3, 5, 6B, and 8 were considerably correlated in the elderly. As discussed earlier, this event may be observed when the assay is not specific, and may be due to an antigenic stimulus common to these serotypes, most likely a cross-reactive antigen.…”

Section: Discussionsupporting

confidence: 88%

“…As discussed earlier, this event may be observed when the assay is not specific, and may be due to an antigenic stimulus common to these serotypes, most likely a cross-reactive antigen. On the other hand, serotype 14 showed the lowest correlation coefficient, confirming the considerably type-specific measurement of antibodies to serotype 14 by ELISA demonstrated in several studies (13,17,22,23,25). However, after vaccination, all pairwise correlations showed a consistent decrease, with the exception of serotype 14.…”

Section: Discussionsupporting

confidence: 78%

“…These data suggest an improvement in antibody specificity to serotypes 1, 3, 5, 6B, and 8. As reported by others (13,23,26), lower correlation coefficients for antibody concentration with other serotypes could mean the absence of cross-reactive antigens. The increase of correlation for serotype 14 in post-vaccinal sera may suggest an overall increase in total IgG concentration.…”

Section: Discussionmentioning

confidence: 55%

“…In an attempt to determine if there was a relationship between specific antibody concentrations for any two serotypes, we performed all pairwise comparisons of antibody concentrations for both age groups, as suggested by Coughlin et al (13). In Figure 3, we present the correlation coefficients for pre-and post-immunization sera obtained for each group.…”

Section: Resultsmentioning

confidence: 99%

“…The pneumococcal capsular polysaccharide preparations used in the present ELISA are contaminated with a common cell wall (C-PS), and therefore antibodies to C-PS should be absorbed to improve the specificity of the ELISA (21). However, recent reports have shown that the second-generation ELISA was found to have insufficient specificity when serum samples from unimmunized subjects were analyzed (13). Furthermore, several investigators reported that, despite the C-PS absorption, antibodies cross-reactive with several types of pneumococcal polysaccharides are still measured (17,18,22).…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in Brazilian elderly

Simonsen

Brandão

Brandileone

et al. 2005

Braz J Med Biol Res

View full text Add to dashboard Cite

Serum antibodies specific for the capsular polysaccharides of Streptococcus pneumoniae provide protection against invasive pneumococcal infection. In Brazil, this vaccine has been used for people over 65 years with clinical risk to develop pneumococcal infection since 1999. We evaluated the immune response of 102 elderly subjects (75.5% females and 24.5% males) with a mean age of 71 years, and 19 young healthy adults (63.2% females and 36.8% males) with a mean age of 27 years. The elderly study group consisted of outpatients who received follow-up care in the Geriatric Department of General Hospital, Faculty of Medicine, University of São Paulo. None had acute illness at the time of vaccination. Both groups were immunized with one intra-deltoid injection with 0.5 ml of a 23-valent pneumococcal polysaccharide vaccine. The total IgG specific antibody concentrations to capsular polysaccharides 1, 3, 5, 6B, 8, and 14 were determined against pre-and 1-month post-vaccination sera. All samples were analyzed according to the second-generation pneumococcal polysaccharide ELISA protocol. We observed that the pneumococcal polysaccharide vaccine evoked consistent antibody increase for serotypes 1, 5, 6B, 8, and 14 (geometric mean concentration increase of 2.46 in the elderly and 2.84 in the young adults). Otherwise, we observed no increase in antibody concentration for serotype 3 in both groups.

show abstract

Section: Discussionsupporting

confidence: 88%